Phenotypic characterization of pre-harvest sprouting resistance mutants generated by the CRISPR/Cas9-geminiviral replicon system in rice
BMB Rep. 2024 Feb 5:6108. Online ahead of print.ABSTRACTPre-harvest sprouting is a critical phenomenon involving germination of seeds in the mother plant before harvest under relative humid conditions and reduced dormancy. In this paper, we generated HDR mutant lines with one region SNP (C/T) and an insertion of 6 bp (GGT/GGTGGCGGC) in OsERF1 genes for pre-harvest sprouting (PHS) resistance using CRISPR/Cas9 and a geminiviral replicon system. The incidence of HDR was 2.6% in transformed calli. T1 seeds were harvested from 12 HDR-induced calli and named ERF1-hdr line. Molecular stability, key agronomic properties, physiolog...
Source: BMB Reports - February 2, 2024 Category: Biochemistry Authors: Jong Hee Kim Jihyeon Yu Jin Young Kim Yong Jin Park Sangsu Bae Kwon Kyoo Kang Yu Jin Jung Source Type: research
Gefitinib induces anoikis in cervical cancer cells
BMB Rep. 2024 Feb 5:6127. Online ahead of print.ABSTRACTGefitinib exerts anticancer effects on various types of cancer, such as lung, ovarian, breast, and colon cancers. However, the therapeutic effects of gefitinib on cervical cancer and the underlying mechanisms remain unclear. Thus, this study aimed to explore whether gefitinib can be used to treat cervical cancer and elucidate the underlying mechanisms. Results showed that gefitinib induced a caspase-dependent apoptosis of HeLa cells, which consequently became round and detached from the surface of the culture plate. Gefitinib induced the reorganization of actin cytosk...
Source: BMB Reports - February 2, 2024 Category: Biochemistry Authors: Byung Chul Jung Sung-Hun Woo Sung Hoon Kim Yoon Suk Kim Source Type: research
Calnexin as a dual-role biomarker: antibody-based diagnosis and therapeutic targeting in lung cancer
In conclusion, this study establishes calnexin as a promising target for antibody-based lung cancer diagnosis and therapy, unlocking novel avenues for early detection and treatment.PMID:38303563 (Source: BMB Reports)
Source: BMB Reports - February 2, 2024 Category: Biochemistry Authors: Soyeon Lim Youngeun Ha Boram Lee Junho Shin Taiyoun Rhim Source Type: research
Therapeutic potential of BMSC-conditioned medium in an in vitro model of renal fibrosis using the RPTEC/TERT1 cell line
BMB Rep. 2024 Feb 5:6149. Online ahead of print.ABSTRACTWe investigated the therapeutic potential of bone marrow-derived mesenchymal stem cell-conditioned medium (BMSC-CM) on immortalized renal proximal tubule epithelial cells (RPTEC/ TERT1) in a fibrotic environment. To replicate the increased stiffness characteristic of kidneys in chronic kidney disease, we utilized polyacrylamide gel platforms. A stiff matrix was shown to increase α-smooth muscle actin (α-SMA) levels, indicating fibrogenic activation in RPTEC/TERT1 cells. Interestingly, treatment with BMSC-CM resulted in significant reductions in the levels of fibroti...
Source: BMB Reports - February 2, 2024 Category: Biochemistry Authors: Yunji Kim Dayeon Kang Ga-Eun Choi Sang Dae Kim Sun-Ja Yang Hyosang Kim Dalsan You Choung Soo Kim Nayoung Suh Source Type: research
Current status of genome editing technologies: special issue of BMB Reports in 2024
BMB Rep. 2024 Jan;57(1):1. doi: 10.5483/BMBRep.2023-0248.ABSTRACTSince the identification of DNA as a genetic material, manipulating DNA in various organisms has been a long standing dream of humanity. In pursuit of this objective, technologies to edit genome have been extensively developed over the recent decades. The emergence of zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) systems enabled site-specific DNA cleavage in a programmable manner. Furthermore, the advent of base editors (BEs...
Source: BMB Reports - January 5, 2024 Category: Biochemistry Authors: Ju-Chan Park Sangsu Bae Source Type: research
Applications of CRISPR technologies to the development of gene and cell therapy
BMB Rep. 2024 Jan;57(1):2-11. doi: 10.5483/BMBRep.2023-0221.ABSTRACTAdvancements in gene and cell therapy have resulted in novel therapeutics for diseases previously considered incurable or challenging to treat. Among the various contributing technologies, genome editing stands out as one of the most crucial for the progress in gene and cell therapy. The discovery of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and the subsequent evolution of genetic engineering technology have markedly expanded the field of target-specific gene editing. Originally studied in the immune systems of bacteria and archaea...
Source: BMB Reports - January 5, 2024 Category: Biochemistry Authors: Chul-Sung Park Omer Habib Younsu Lee Junho K Hur Source Type: research
Mitochondrial genome editing: strategies, challenges, and applications
BMB Rep. 2024 Jan;57(1):19-29. doi: 10.5483/BMBRep.2023-0224.ABSTRACTMitochondrial DNA (mtDNA), a multicopy genome found in mitochondria, is crucial for oxidative phosphorylation. Mutations in mtDNA can lead to severe mitochondrial dysfunction in tissues and organs with high energy demand. MtDNA mutations are closely associated with mitochondrial and age-related disease. To better understand the functional role of mtDNA and work toward developing therapeutics, it is essential to advance technology that is capable of manipulating the mitochondrial genome. This review discusses ongoing efforts in mitochondrial genome editing...
Source: BMB Reports - January 5, 2024 Category: Biochemistry Authors: Kayeong Lim Source Type: research
Current status of genome editing technologies: special issue of BMB Reports in 2024
BMB Rep. 2024 Jan 5:6162. Online ahead of print.ABSTRACTSince the identification of DNA as a genetic material, manipulating DNA in various organisms has been a long standing dream of humanity. In pursuit of this objective, technologies to edit genome have been extensively developed over the recent decades. The emergence of zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) systems enabled site-specific DNA cleavage in a programmable manner. Furthermore, the advent of base editors (BEs) and pri...
Source: BMB Reports - January 5, 2024 Category: Biochemistry Authors: Ju-Chan Park Sangsu Bae Source Type: research
Applications of CRISPR technologies to the development of gene and cell therapy
BMB Rep. 2024 Jan 5:6122. Online ahead of print.ABSTRACTAdvancements in gene and cell therapy have resulted in novel therapeutics for diseases previously considered incurable or challenging to treat. Among the various contributing technologies, genome editing stands out as one of the most crucial for the progress in gene and cell therapy. The discovery of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and the subsequent evolution of genetic engineering technology have markedly expanded the field of target-specific gene editing. Originally studied in the immune systems of bacteria and archaea, the CRISPR...
Source: BMB Reports - January 5, 2024 Category: Biochemistry Authors: Chul-Sung Park Omer Habib Younsu Lee Junho K Hur Source Type: research
Mitochondrial genome editing: strategies, challenges, and applications
BMB Rep. 2024 Jan 5:6125. Online ahead of print.ABSTRACTMitochondrial DNA (mtDNA), a multicopy genome found in mitochondria, is crucial for oxidative phosphorylation. Mutations in mtDNA can lead to severe mitochondrial dysfunction in tissues and organs with high energy demand. MtDNA mutations are closely associated with mitochondrial and age-related disease. To better understand the functional role of mtDNA and work toward developing therapeutics, it is essential to advance technology that is capable of manipulating the mitochondrial genome. This review discusses ongoing efforts in mitochondrial genome editing with mtDNA n...
Source: BMB Reports - January 5, 2024 Category: Biochemistry Authors: Kayeong Lim Source Type: research
Current status of genome editing technologies: special issue of BMB Reports in 2024
BMB Rep. 2024 Jan 5:6162. Online ahead of print.ABSTRACTSince the identification of DNA as a genetic material, manipulating DNA in various organisms has been a long standing dream of humanity. In pursuit of this objective, technologies to edit genome have been extensively developed over the recent decades. The emergence of zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) systems enabled site-specific DNA cleavage in a programmable manner. Furthermore, the advent of base editors (BEs) and pri...
Source: BMB Reports - January 5, 2024 Category: Biochemistry Authors: Ju-Chan Park Sangsu Bae Source Type: research
Applications of CRISPR technologies to the development of gene and cell therapy
BMB Rep. 2024 Jan 5:6122. Online ahead of print.ABSTRACTAdvancements in gene and cell therapy have resulted in novel therapeutics for diseases previously considered incurable or challenging to treat. Among the various contributing technologies, genome editing stands out as one of the most crucial for the progress in gene and cell therapy. The discovery of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and the subsequent evolution of genetic engineering technology have markedly expanded the field of target-specific gene editing. Originally studied in the immune systems of bacteria and archaea, the CRISPR...
Source: BMB Reports - January 5, 2024 Category: Biochemistry Authors: Chul-Sung Park Omer Habib Younsu Lee Junho K Hur Source Type: research
Mitochondrial genome editing: strategies, challenges, and applications
BMB Rep. 2024 Jan 5:6125. Online ahead of print.ABSTRACTMitochondrial DNA (mtDNA), a multicopy genome found in mitochondria, is crucial for oxidative phosphorylation. Mutations in mtDNA can lead to severe mitochondrial dysfunction in tissues and organs with high energy demand. MtDNA mutations are closely associated with mitochondrial and age-related disease. To better understand the functional role of mtDNA and work toward developing therapeutics, it is essential to advance technology that is capable of manipulating the mitochondrial genome. This review discusses ongoing efforts in mitochondrial genome editing with mtDNA n...
Source: BMB Reports - January 5, 2024 Category: Biochemistry Authors: Kayeong Lim Source Type: research
Current status of genome editing technologies: special issue of BMB Reports in 2024
BMB Rep. 2024 Jan 5:6162. Online ahead of print.ABSTRACTSince the identification of DNA as a genetic material, manipulating DNA in various organisms has been a long standing dream of humanity. In pursuit of this objective, technologies to edit genome have been extensively developed over the recent decades. The emergence of zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) systems enabled site-specific DNA cleavage in a programmable manner. Furthermore, the advent of base editors (BEs) and pri...
Source: BMB Reports - January 5, 2024 Category: Biochemistry Authors: Ju-Chan Park Sangsu Bae Source Type: research
Applications of CRISPR technologies to the development of gene and cell therapy
BMB Rep. 2024 Jan 5:6122. Online ahead of print.ABSTRACTAdvancements in gene and cell therapy have resulted in novel therapeutics for diseases previously considered incurable or challenging to treat. Among the various contributing technologies, genome editing stands out as one of the most crucial for the progress in gene and cell therapy. The discovery of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and the subsequent evolution of genetic engineering technology have markedly expanded the field of target-specific gene editing. Originally studied in the immune systems of bacteria and archaea, the CRISPR...
Source: BMB Reports - January 5, 2024 Category: Biochemistry Authors: Chul-Sung Park Omer Habib Younsu Lee Junho K Hur Source Type: research
