Therapeutic potential of BMSC-conditioned medium in an in vitro model of renal fibrosis using the RPTEC/TERT1 cell line
BMB Rep. 2024 Feb 5:6149. Online ahead of print.ABSTRACTWe investigated the therapeutic potential of bone marrow-derived mesenchymal stem cell-conditioned medium (BMSC-CM) on immortalized renal proximal tubule epithelial cells (RPTEC/ TERT1) in a fibrotic environment. To replicate the increased stiffness characteristic of kidneys in chronic kidney disease, we utilized polyacrylamide gel platforms. A stiff matrix was shown to increase α-smooth muscle actin (α-SMA) levels, indicating fibrogenic activation in RPTEC/TERT1 cells. Interestingly, treatment with BMSC-CM resulted in significant reductions in the levels of fibroti...
Source: BMB Reports - February 2, 2024 Category: Biochemistry Authors: Yunji Kim Dayeon Kang Ga-Eun Choi Sang Dae Kim Sun-Ja Yang Hyosang Kim Dalsan You Choung Soo Kim Nayoung Suh Source Type: research
Loss of hepatic Sirt7 accelerates diethylnitrosamine (DEN)-induced formation of hepatocellular carcinoma by impairing DNA damage repair
BMB Rep. 2024 Feb 5:6076. Online ahead of print.ABSTRACTThe mammalian sirtuin family (SIRT1-SIRT7) has shown diverse biological roles in the regulation and maintenance of genome stability under genotoxic stress. SIRT7, one of the least studied sirtuin, has been demonstrated to be a key factor for DNA damage response (DDR). However, conflicting results have proposed that Sirt7 is an oncogenic factor to promote transformation in cancer cells. To address this inconsistency, we investigated properties of SIRT7 in hepatocellular carcinoma (HCC) regulation under DNA damage and found that loss of hepatic Sirt7 accelerated HCC pro...
Source: BMB Reports - February 2, 2024 Category: Biochemistry Authors: Yuna Kim Baeki E Kang Karim Gariani Joanna Gariani Junguee Lee Hyun-Jin Kim Chang-Woo Lee Kristina Schoonjans Johan Auwerx Dongryeol Ryu Source Type: research
Phenotypic characterization of pre-harvest sprouting resistance mutants generated by the CRISPR/Cas9-geminiviral replicon system in rice
BMB Rep. 2024 Feb 5:6108. Online ahead of print.ABSTRACTPre-harvest sprouting is a critical phenomenon involving germination of seeds in the mother plant before harvest under relative humid conditions and reduced dormancy. In this paper, we generated HDR mutant lines with one region SNP (C/T) and an insertion of 6 bp (GGT/GGTGGCGGC) in OsERF1 genes for pre-harvest sprouting (PHS) resistance using CRISPR/Cas9 and a geminiviral replicon system. The incidence of HDR was 2.6% in transformed calli. T1 seeds were harvested from 12 HDR-induced calli and named ERF1-hdr line. Molecular stability, key agronomic properties, physiolog...
Source: BMB Reports - February 2, 2024 Category: Biochemistry Authors: Jong Hee Kim Jihyeon Yu Jin Young Kim Yong Jin Park Sangsu Bae Kwon Kyoo Kang Yu Jin Jung Source Type: research
Gefitinib induces anoikis in cervical cancer cells
BMB Rep. 2024 Feb 5:6127. Online ahead of print.ABSTRACTGefitinib exerts anticancer effects on various types of cancer, such as lung, ovarian, breast, and colon cancers. However, the therapeutic effects of gefitinib on cervical cancer and the underlying mechanisms remain unclear. Thus, this study aimed to explore whether gefitinib can be used to treat cervical cancer and elucidate the underlying mechanisms. Results showed that gefitinib induced a caspase-dependent apoptosis of HeLa cells, which consequently became round and detached from the surface of the culture plate. Gefitinib induced the reorganization of actin cytosk...
Source: BMB Reports - February 2, 2024 Category: Biochemistry Authors: Byung Chul Jung Sung-Hun Woo Sung Hoon Kim Yoon Suk Kim Source Type: research
Calnexin as a dual-role biomarker: antibody-based diagnosis and therapeutic targeting in lung cancer
In conclusion, this study establishes calnexin as a promising target for antibody-based lung cancer diagnosis and therapy, unlocking novel avenues for early detection and treatment.PMID:38303563 (Source: BMB Reports)
Source: BMB Reports - February 2, 2024 Category: Biochemistry Authors: Soyeon Lim Youngeun Ha Boram Lee Junho Shin Taiyoun Rhim Source Type: research
Therapeutic potential of BMSC-conditioned medium in an in vitro model of renal fibrosis using the RPTEC/TERT1 cell line
BMB Rep. 2024 Feb 5:6149. Online ahead of print.ABSTRACTWe investigated the therapeutic potential of bone marrow-derived mesenchymal stem cell-conditioned medium (BMSC-CM) on immortalized renal proximal tubule epithelial cells (RPTEC/ TERT1) in a fibrotic environment. To replicate the increased stiffness characteristic of kidneys in chronic kidney disease, we utilized polyacrylamide gel platforms. A stiff matrix was shown to increase α-smooth muscle actin (α-SMA) levels, indicating fibrogenic activation in RPTEC/TERT1 cells. Interestingly, treatment with BMSC-CM resulted in significant reductions in the levels of fibroti...
Source: BMB Reports - February 2, 2024 Category: Biochemistry Authors: Yunji Kim Dayeon Kang Ga-Eun Choi Sang Dae Kim Sun-Ja Yang Hyosang Kim Dalsan You Choung Soo Kim Nayoung Suh Source Type: research
Loss of hepatic Sirt7 accelerates diethylnitrosamine (DEN)-induced formation of hepatocellular carcinoma by impairing DNA damage repair
BMB Rep. 2024 Feb 5:6076. Online ahead of print.ABSTRACTThe mammalian sirtuin family (SIRT1-SIRT7) has shown diverse biological roles in the regulation and maintenance of genome stability under genotoxic stress. SIRT7, one of the least studied sirtuin, has been demonstrated to be a key factor for DNA damage response (DDR). However, conflicting results have proposed that Sirt7 is an oncogenic factor to promote transformation in cancer cells. To address this inconsistency, we investigated properties of SIRT7 in hepatocellular carcinoma (HCC) regulation under DNA damage and found that loss of hepatic Sirt7 accelerated HCC pro...
Source: BMB Reports - February 2, 2024 Category: Biochemistry Authors: Yuna Kim Baeki E Kang Karim Gariani Joanna Gariani Junguee Lee Hyun-Jin Kim Chang-Woo Lee Kristina Schoonjans Johan Auwerx Dongryeol Ryu Source Type: research
Phenotypic characterization of pre-harvest sprouting resistance mutants generated by the CRISPR/Cas9-geminiviral replicon system in rice
BMB Rep. 2024 Feb 5:6108. Online ahead of print.ABSTRACTPre-harvest sprouting is a critical phenomenon involving germination of seeds in the mother plant before harvest under relative humid conditions and reduced dormancy. In this paper, we generated HDR mutant lines with one region SNP (C/T) and an insertion of 6 bp (GGT/GGTGGCGGC) in OsERF1 genes for pre-harvest sprouting (PHS) resistance using CRISPR/Cas9 and a geminiviral replicon system. The incidence of HDR was 2.6% in transformed calli. T1 seeds were harvested from 12 HDR-induced calli and named ERF1-hdr line. Molecular stability, key agronomic properties, physiolog...
Source: BMB Reports - February 2, 2024 Category: Biochemistry Authors: Jong Hee Kim Jihyeon Yu Jin Young Kim Yong Jin Park Sangsu Bae Kwon Kyoo Kang Yu Jin Jung Source Type: research
Gefitinib induces anoikis in cervical cancer cells
BMB Rep. 2024 Feb 5:6127. Online ahead of print.ABSTRACTGefitinib exerts anticancer effects on various types of cancer, such as lung, ovarian, breast, and colon cancers. However, the therapeutic effects of gefitinib on cervical cancer and the underlying mechanisms remain unclear. Thus, this study aimed to explore whether gefitinib can be used to treat cervical cancer and elucidate the underlying mechanisms. Results showed that gefitinib induced a caspase-dependent apoptosis of HeLa cells, which consequently became round and detached from the surface of the culture plate. Gefitinib induced the reorganization of actin cytosk...
Source: BMB Reports - February 2, 2024 Category: Biochemistry Authors: Byung Chul Jung Sung-Hun Woo Sung Hoon Kim Yoon Suk Kim Source Type: research
Calnexin as a dual-role biomarker: antibody-based diagnosis and therapeutic targeting in lung cancer
In conclusion, this study establishes calnexin as a promising target for antibody-based lung cancer diagnosis and therapy, unlocking novel avenues for early detection and treatment.PMID:38303563 (Source: BMB Reports)
Source: BMB Reports - February 2, 2024 Category: Biochemistry Authors: Soyeon Lim Youngeun Ha Boram Lee Junho Shin Taiyoun Rhim Source Type: research
Therapeutic potential of BMSC-conditioned medium in an in vitro model of renal fibrosis using the RPTEC/TERT1 cell line
BMB Rep. 2024 Feb 5:6149. Online ahead of print.ABSTRACTWe investigated the therapeutic potential of bone marrow-derived mesenchymal stem cell-conditioned medium (BMSC-CM) on immortalized renal proximal tubule epithelial cells (RPTEC/ TERT1) in a fibrotic environment. To replicate the increased stiffness characteristic of kidneys in chronic kidney disease, we utilized polyacrylamide gel platforms. A stiff matrix was shown to increase α-smooth muscle actin (α-SMA) levels, indicating fibrogenic activation in RPTEC/TERT1 cells. Interestingly, treatment with BMSC-CM resulted in significant reductions in the levels of fibroti...
Source: BMB Reports - February 2, 2024 Category: Biochemistry Authors: Yunji Kim Dayeon Kang Ga-Eun Choi Sang Dae Kim Sun-Ja Yang Hyosang Kim Dalsan You Choung Soo Kim Nayoung Suh Source Type: research
Loss of hepatic Sirt7 accelerates diethylnitrosamine (DEN)-induced formation of hepatocellular carcinoma by impairing DNA damage repair
BMB Rep. 2024 Feb 5:6076. Online ahead of print.ABSTRACTThe mammalian sirtuin family (SIRT1-SIRT7) has shown diverse biological roles in the regulation and maintenance of genome stability under genotoxic stress. SIRT7, one of the least studied sirtuin, has been demonstrated to be a key factor for DNA damage response (DDR). However, conflicting results have proposed that Sirt7 is an oncogenic factor to promote transformation in cancer cells. To address this inconsistency, we investigated properties of SIRT7 in hepatocellular carcinoma (HCC) regulation under DNA damage and found that loss of hepatic Sirt7 accelerated HCC pro...
Source: BMB Reports - February 2, 2024 Category: Biochemistry Authors: Yuna Kim Baeki E Kang Karim Gariani Joanna Gariani Junguee Lee Hyun-Jin Kim Chang-Woo Lee Kristina Schoonjans Johan Auwerx Dongryeol Ryu Source Type: research
Phenotypic characterization of pre-harvest sprouting resistance mutants generated by the CRISPR/Cas9-geminiviral replicon system in rice
BMB Rep. 2024 Feb 5:6108. Online ahead of print.ABSTRACTPre-harvest sprouting is a critical phenomenon involving germination of seeds in the mother plant before harvest under relative humid conditions and reduced dormancy. In this paper, we generated HDR mutant lines with one region SNP (C/T) and an insertion of 6 bp (GGT/GGTGGCGGC) in OsERF1 genes for pre-harvest sprouting (PHS) resistance using CRISPR/Cas9 and a geminiviral replicon system. The incidence of HDR was 2.6% in transformed calli. T1 seeds were harvested from 12 HDR-induced calli and named ERF1-hdr line. Molecular stability, key agronomic properties, physiolog...
Source: BMB Reports - February 2, 2024 Category: Biochemistry Authors: Jong Hee Kim Jihyeon Yu Jin Young Kim Yong Jin Park Sangsu Bae Kwon Kyoo Kang Yu Jin Jung Source Type: research
Gefitinib induces anoikis in cervical cancer cells
BMB Rep. 2024 Feb 5:6127. Online ahead of print.ABSTRACTGefitinib exerts anticancer effects on various types of cancer, such as lung, ovarian, breast, and colon cancers. However, the therapeutic effects of gefitinib on cervical cancer and the underlying mechanisms remain unclear. Thus, this study aimed to explore whether gefitinib can be used to treat cervical cancer and elucidate the underlying mechanisms. Results showed that gefitinib induced a caspase-dependent apoptosis of HeLa cells, which consequently became round and detached from the surface of the culture plate. Gefitinib induced the reorganization of actin cytosk...
Source: BMB Reports - February 2, 2024 Category: Biochemistry Authors: Byung Chul Jung Sung-Hun Woo Sung Hoon Kim Yoon Suk Kim Source Type: research
Calnexin as a dual-role biomarker: antibody-based diagnosis and therapeutic targeting in lung cancer
In conclusion, this study establishes calnexin as a promising target for antibody-based lung cancer diagnosis and therapy, unlocking novel avenues for early detection and treatment.PMID:38303563 (Source: BMB Reports)
Source: BMB Reports - February 2, 2024 Category: Biochemistry Authors: Soyeon Lim Youngeun Ha Boram Lee Junho Shin Taiyoun Rhim Source Type: research
