Sodium butyrate ameliorates high glucose-suppressed neuronal mitophagy by restoring PRKN expression via inhibiting the RELA-HDAC8 complex
Autophagy. 2024 Feb 26. doi: 10.1080/15548627.2024.2323785. Online ahead of print.ABSTRACTDamaged mitochondria accumulation in diabetes is one of the main features that contribute to increased incidence of cognitive impairment by inducing apoptosis. Butyrate is a major metabolite produced by microbiota that has neuroprotective effects by regulating mitochondrial function. However, detailed mechanisms underlying how butyrate can regulate neuronal mitophagy remain unclear. Here, we examined the regulatory effects of sodium butyrate (NaB) on high glucose-induced mitophagy dysregulation, neuronal apoptosis, and cognitive impai...
Source: Autophagy - February 27, 2024 Category: Cytology Authors: Ji Hyeon Cho Chang Woo Chae Jae Ryong Lim Young Hyun Jung Su Jong Han Jee Hyeon Yoon Ji Yong Park Ho Jae Han Source Type: research
The HSP90 chaperone code regulates the crosstalk between proteostasis and autophagy
Autophagy. 2024 Feb 27. doi: 10.1080/15548627.2024.2323259. Online ahead of print.ABSTRACTProteostasis, the maintenance of proper protein folding, stability, and degradation within cells, is fundamental for cellular function. Two key players in this intricate cellular process are macroautophagy/autophagy and chaperoning of nascent proteins. Here, we explore the crosstalk between autophagy and the HSP90 chaperone in maintaining proteostasis, highlighting their interplay and significance in cellular homeostasis.PMID:38411135 | DOI:10.1080/15548627.2024.2323259 (Source: Autophagy)
Source: Autophagy - February 27, 2024 Category: Cytology Authors: Sarah J Backe Jennifer A Heritz Mehdi Mollapour Source Type: research
PtdIns4p is required for the autophagosomal recruitment of STX17 (syntaxin 17) to promote lysosomal fusion
Autophagy. 2024 Feb 27. doi: 10.1080/15548627.2024.2322493. Online ahead of print.ABSTRACTThe autophagosomal SNARE STX17 (syntaxin 17) promotes lysosomal fusion and degradation, but its autophagosomal recruitment is incompletely understood. Notably, PtdIns4P is generated on autophagosomes and promotes fusion through an unknown mechanism. Here we show that soluble recombinant STX17 is spontaneously recruited to negatively charged liposomes and adding PtdIns4P to liposomes containing neutral lipids is sufficient for its recruitment. Consistently, STX17 colocalizes with PtdIns4P-positive autophagosomes in cells, and specific ...
Source: Autophagy - February 27, 2024 Category: Cytology Authors: Hajnalka Laczk ó-Dobos Arindam Bhattacharjee Asha Kiran Maddali Andr ás Kincses Hussein Abuammar Krisztina Seb ők-Nagy Tibor P áli Andr ás Dér Tam ás Hegedűs G ábor Csordás G ábor Juhász Source Type: research
Neuronal waste management: new roles for autophagy genes in the extrusion of protein aggregates and in longevity
Autophagy. 2024 Feb 27. doi: 10.1080/15548627.2024.2322420. Online ahead of print.ABSTRACTA decline in macroautophagic/autophagic activity with age contributes to the accumulation of damaged molecules and is associated with the impairment of neuronal functions and the onset of age-related diseases, particularly neurodegenerative disorders. To learn about the neuronal-specific roles of autophagy genes in aging, we specifically inhibited autophagy genes pan-neuronally in C. elegans, which leads to unexpected positive impacts on neuronal homeostasis including polyQ aggregate load and organismal lifespan. These improvements ar...
Source: Autophagy - February 27, 2024 Category: Cytology Authors: Ling-Hsuan Sun Caitlin Lange Malene Hansen Caroline Kumsta Source Type: research
Aging STINGs: mitophagy at the crossroads of neuroinflammation
Autophagy. 2024 Feb 27. doi: 10.1080/15548627.2024.2322421. Online ahead of print.ABSTRACTLoss of proteostasis and dysregulated mitochondrial function are part of the traditional hallmarks of aging, and in their last revision impaired macroautophagy and chronic inflammation are also included. Mitophagy is at the intersection of all these processes but whether it undergoes age-associated perturbations was not known. In our recent work, we performed a systematic and systemic analysis of mitolysosome levels in mice and found that, despite the already-known decrease in non-selective macroautophagy, mitophagy remains stable or ...
Source: Autophagy - February 27, 2024 Category: Cytology Authors: Juan Ignacio Jim énez-Loygorri Patricia Boya Source Type: research
TRIM27 elicits protective immunity against tuberculosis by activating TFEB-mediated autophagy flux
Autophagy. 2024 Feb 23. doi: 10.1080/15548627.2024.2321831. Online ahead of print.ABSTRACTInfectious diseases, such as Mycobacterium tuberculosis (Mtb)-caused tuberculosis (TB), remain a global threat exacerbated by increasing drug resistance. Host-directed therapy (HDT) is a promising strategy for infection treatment through targeting host immunity. However, the limited understanding of the function and regulatory mechanism of host factors involved in immune defense against infections has impeded HDT development. Here, we identify the ubiquitin ligase (E3) TRIM27 (tripartite motif-containing 27) as a host protective facto...
Source: Autophagy - February 23, 2024 Category: Cytology Authors: Dongdong Zhao Lihua Qiang Zehui Lei Pupu Ge Zhe Lu Yiru Wang Xinwen Zhang Yuyun Qiang Bingxi Li Yu Pang Lingqiang Zhang Cui Hua Liu Jing Wang Source Type: research
TRIM27 elicits protective immunity against tuberculosis by activating TFEB-mediated autophagy flux
Autophagy. 2024 Feb 23. doi: 10.1080/15548627.2024.2321831. Online ahead of print.ABSTRACTInfectious diseases, such as Mycobacterium tuberculosis (Mtb)-caused tuberculosis (TB), remain a global threat exacerbated by increasing drug resistance. Host-directed therapy (HDT) is a promising strategy for infection treatment through targeting host immunity. However, the limited understanding of the function and regulatory mechanism of host factors involved in immune defense against infections has impeded HDT development. Here, we identify the ubiquitin ligase (E3) TRIM27 (tripartite motif-containing 27) as a host protective facto...
Source: Autophagy - February 23, 2024 Category: Cytology Authors: Dongdong Zhao Lihua Qiang Zehui Lei Pupu Ge Zhe Lu Yiru Wang Xinwen Zhang Yuyun Qiang Bingxi Li Yu Pang Lingqiang Zhang Cui Hua Liu Jing Wang Source Type: research
TRIM27 elicits protective immunity against tuberculosis by activating TFEB-mediated autophagy flux
Autophagy. 2024 Feb 23. doi: 10.1080/15548627.2024.2321831. Online ahead of print.ABSTRACTInfectious diseases, such as Mycobacterium tuberculosis (Mtb)-caused tuberculosis (TB), remain a global threat exacerbated by increasing drug resistance. Host-directed therapy (HDT) is a promising strategy for infection treatment through targeting host immunity. However, the limited understanding of the function and regulatory mechanism of host factors involved in immune defense against infections has impeded HDT development. Here, we identify the ubiquitin ligase (E3) TRIM27 (tripartite motif-containing 27) as a host protective facto...
Source: Autophagy - February 23, 2024 Category: Cytology Authors: Dongdong Zhao Lihua Qiang Zehui Lei Pupu Ge Zhe Lu Yiru Wang Xinwen Zhang Yuyun Qiang Bingxi Li Yu Pang Lingqiang Zhang Cui Hua Liu Jing Wang Source Type: research
TRIM27 elicits protective immunity against tuberculosis by activating TFEB-mediated autophagy flux
Autophagy. 2024 Feb 23. doi: 10.1080/15548627.2024.2321831. Online ahead of print.ABSTRACTInfectious diseases, such as Mycobacterium tuberculosis (Mtb)-caused tuberculosis (TB), remain a global threat exacerbated by increasing drug resistance. Host-directed therapy (HDT) is a promising strategy for infection treatment through targeting host immunity. However, the limited understanding of the function and regulatory mechanism of host factors involved in immune defense against infections has impeded HDT development. Here, we identify the ubiquitin ligase (E3) TRIM27 (tripartite motif-containing 27) as a host protective facto...
Source: Autophagy - February 23, 2024 Category: Cytology Authors: Dongdong Zhao Lihua Qiang Zehui Lei Pupu Ge Zhe Lu Yiru Wang Xinwen Zhang Yuyun Qiang Bingxi Li Yu Pang Lingqiang Zhang Cui Hua Liu Jing Wang Source Type: research
Selective degradation of ribosomes during oncogene-induced senescence: molecular insights and biological perspectives
Autophagy. 2024 Feb 21:1-3. doi: 10.1080/15548627.2024.2319022. Online ahead of print.ABSTRACTRibosomes are conserved macromolecular machines that are responsible for protein synthesis in all cells. While our knowledge of ribosome biogenesis and function has increased significantly in recent years, little is known about how ribosomes are degraded under specific cellular conditions. We recently uncovered that ribosomes are efficiently turned over by selective macroautophagy/autophagy during oncogene-induced senescence (OIS). By profiling the ribosome interactome in human fibroblasts undergoing OIS, we discovered a key role ...
Source: Autophagy - February 21, 2024 Category: Cytology Authors: Aida Rodr íguez López Lisa B Frankel Source Type: research
Selective degradation of ribosomes during oncogene-induced senescence: molecular insights and biological perspectives
Autophagy. 2024 Feb 21:1-3. doi: 10.1080/15548627.2024.2319022. Online ahead of print.ABSTRACTRibosomes are conserved macromolecular machines that are responsible for protein synthesis in all cells. While our knowledge of ribosome biogenesis and function has increased significantly in recent years, little is known about how ribosomes are degraded under specific cellular conditions. We recently uncovered that ribosomes are efficiently turned over by selective macroautophagy/autophagy during oncogene-induced senescence (OIS). By profiling the ribosome interactome in human fibroblasts undergoing OIS, we discovered a key role ...
Source: Autophagy - February 21, 2024 Category: Cytology Authors: Aida Rodr íguez López Lisa B Frankel Source Type: research
ATG5-regulated CCL2/MCP-1 production in myeloid cells selectively modulates anti-malarial CD4 < sup > + < /sup > Th1 responses
This study demonstrated that ATG5 deficiency in myeloid cells can significantly inhibit the growth of rodent blood-stage malarial parasites by selectively enhancing parasite-specific CD4+ Th1 cell responses. This effect was independent of ATG5-mediated canonical and non-canonical autophagy. Mechanistically, ATG5 deficiency suppressed FAS-mediated apoptosis of LY6G- ITGAM/CD11b+ ADGRE1/F4/80- cells and subsequently increased CCL2/MCP-1 production in parasite-infected mice. LY6G- ITGAM+ ADGRE1- cell-derived CCL2 selectively interacted with CCR2 on CD4+ Th1 cells for their optimized responses through the JAK2-STAT4 pathway. T...
Source: Autophagy - February 18, 2024 Category: Cytology Authors: Yuanli Gao Suilin Chen Shiming Jiao Yongling Fan Xiuxiu Li Nie Tan Jiaqin Fang Luming Xu Yi Huang Jing Zhao Shuai Guo Taiping Liu Wenyue Xu Source Type: research
ATG5-regulated CCL2/MCP-1 production in myeloid cells selectively modulates anti-malarial CD4 < sup > + < /sup > Th1 responses
This study demonstrated that ATG5 deficiency in myeloid cells can significantly inhibit the growth of rodent blood-stage malarial parasites by selectively enhancing parasite-specific CD4+ Th1 cell responses. This effect was independent of ATG5-mediated canonical and non-canonical autophagy. Mechanistically, ATG5 deficiency suppressed FAS-mediated apoptosis of LY6G- ITGAM/CD11b+ ADGRE1/F4/80- cells and subsequently increased CCL2/MCP-1 production in parasite-infected mice. LY6G- ITGAM+ ADGRE1- cell-derived CCL2 selectively interacted with CCR2 on CD4+ Th1 cells for their optimized responses through the JAK2-STAT4 pathway. T...
Source: Autophagy - February 18, 2024 Category: Cytology Authors: Yuanli Gao Suilin Chen Shiming Jiao Yongling Fan Xiuxiu Li Nie Tan Jiaqin Fang Luming Xu Yi Huang Jing Zhao Shuai Guo Taiping Liu Wenyue Xu Source Type: research
ATG5-regulated CCL2/MCP-1 production in myeloid cells selectively modulates anti-malarial CD4 < sup > + < /sup > Th1 responses
This study demonstrated that ATG5 deficiency in myeloid cells can significantly inhibit the growth of rodent blood-stage malarial parasites by selectively enhancing parasite-specific CD4+ Th1 cell responses. This effect was independent of ATG5-mediated canonical and non-canonical autophagy. Mechanistically, ATG5 deficiency suppressed FAS-mediated apoptosis of LY6G- ITGAM/CD11b+ ADGRE1/F4/80- cells and subsequently increased CCL2/MCP-1 production in parasite-infected mice. LY6G- ITGAM+ ADGRE1- cell-derived CCL2 selectively interacted with CCR2 on CD4+ Th1 cells for their optimized responses through the JAK2-STAT4 pathway. T...
Source: Autophagy - February 18, 2024 Category: Cytology Authors: Yuanli Gao Suilin Chen Shiming Jiao Yongling Fan Xiuxiu Li Nie Tan Jiaqin Fang Luming Xu Yi Huang Jing Zhao Shuai Guo Taiping Liu Wenyue Xu Source Type: research
Did mitophagy follow the origin of mitochondria?
Autophagy. 2024 Feb 15:1-9. doi: 10.1080/15548627.2024.2307215. Online ahead of print.ABSTRACTMitophagy is the process of selective autophagy that removes superfluous and dysfunctional mitochondria. Mitophagy was first characterized in mammalian cells and is now recognized to follow several pathways including basal forms in specific organs. Mitophagy pathways are regulated by multiple, often interconnected factors. The present review aims to streamline this complexity and evaluate common elements that may define the evolutionary origin of mitophagy. Key issues surrounding mitophagy signaling at the mitochondrial surface ma...
Source: Autophagy - February 16, 2024 Category: Cytology Authors: Mauro Degli Esposti Source Type: research
