Deacetylation of ATG7 drives the induction of macroautophagy and LC3-associated microautophagy
Autophagy. 2023 Nov 24. doi: 10.1080/15548627.2023.2287932. Online ahead of print.ABSTRACTLC3 lipidation plays an important role in the regulation of macroautophagy and LC3-associated microautophagy. The E1-like enzyme ATG7 is one of the core components that are directly involved in LC3 lipidation reaction. Here, we provide evidence showing that acetylation of ATG7 tightly controls its enzyme activity to regulate the induction of macroautophagy and LC3-associated microautophagy. Mechanistically, acetylation of ATG7 disrupts its interaction with the E2-like enzyme ATG3, leading to an inhibition of LC3 lipidation in vitro an...
Source: Autophagy - November 24, 2023 Category: Cytology Authors: Yinfeng Xu Chuying Qian Qian Wang Lijiang Song Zhengfu He Wei Liu Wei Wan Source Type: research
ULK1 seen at the single-molecule level during autophagy initiation
Autophagy. 2023 Nov 22. doi: 10.1080/15548627.2023.2286078. Online ahead of print.ABSTRACTMacroautophagy/autophagy research often involves overexpressing proteins to investigate their localization, function and activity. However, this approach can disturb the inherent balance of cellular components, potentially affecting the integrity of the autophagy process. With the advent of genome-editing techniques like CRISPR-Cas9, it is now possible to tag endogenous proteins with fluorescent markers, enabling the study of their behaviors under more physiologically relevant conditions. Nevertheless, conventional microscopy methods ...
Source: Autophagy - November 22, 2023 Category: Cytology Authors: Chiranjib Banerjee Elias M Puchner Do-Hyung Kim Source Type: research
Correction of symptoms of Huntington disease by genistein through FOXO3-mediated autophagy stimulation
In conclusion, treatment with genistein stimulates the autophagy process in the brains of HD mice, leading to correction of symptoms of HD, suggesting that it might be considered as a potential drug for this disease. Combined with a very recently published report indicating that impaired autophagy may be a major cause of neurodegenerative changes, these results may indicate the way to the development of effective therapeutic approaches for different neurodegenerative diseases by testing compounds (or possibly combinations of compounds) capable of stimulating autophagy and/or unblocking this process.PMID:37992314 | DOI:10.1...
Source: Autophagy - November 22, 2023 Category: Cytology Authors: Karolina Pierzynowska Magdalena Podlacha Lidia Gaffke Estera Rintz Karolina Wi śniewska Zuzanna Cyske Grzegorz W ęgrzyn Source Type: research
ULK1 seen at the single-molecule level during autophagy initiation
Autophagy. 2023 Nov 22. doi: 10.1080/15548627.2023.2286078. Online ahead of print.ABSTRACTMacroautophagy/autophagy research often involves overexpressing proteins to investigate their localization, function and activity. However, this approach can disturb the inherent balance of cellular components, potentially affecting the integrity of the autophagy process. With the advent of genome-editing techniques like CRISPR-Cas9, it is now possible to tag endogenous proteins with fluorescent markers, enabling the study of their behaviors under more physiologically relevant conditions. Nevertheless, conventional microscopy methods ...
Source: Autophagy - November 22, 2023 Category: Cytology Authors: Chiranjib Banerjee Elias M Puchner Do-Hyung Kim Source Type: research
Correction of symptoms of Huntington disease by genistein through FOXO3-mediated autophagy stimulation
In conclusion, treatment with genistein stimulates the autophagy process in the brains of HD mice, leading to correction of symptoms of HD, suggesting that it might be considered as a potential drug for this disease. Combined with a very recently published report indicating that impaired autophagy may be a major cause of neurodegenerative changes, these results may indicate the way to the development of effective therapeutic approaches for different neurodegenerative diseases by testing compounds (or possibly combinations of compounds) capable of stimulating autophagy and/or unblocking this process.PMID:37992314 | DOI:10.1...
Source: Autophagy - November 22, 2023 Category: Cytology Authors: Karolina Pierzynowska Magdalena Podlacha Lidia Gaffke Estera Rintz Karolina Wi śniewska Zuzanna Cyske Grzegorz W ęgrzyn Source Type: research
ULK1 seen at the single-molecule level during autophagy initiation
Autophagy. 2023 Nov 22. doi: 10.1080/15548627.2023.2286078. Online ahead of print.ABSTRACTMacroautophagy/autophagy research often involves overexpressing proteins to investigate their localization, function and activity. However, this approach can disturb the inherent balance of cellular components, potentially affecting the integrity of the autophagy process. With the advent of genome-editing techniques like CRISPR-Cas9, it is now possible to tag endogenous proteins with fluorescent markers, enabling the study of their behaviors under more physiologically relevant conditions. Nevertheless, conventional microscopy methods ...
Source: Autophagy - November 22, 2023 Category: Cytology Authors: Chiranjib Banerjee Elias M Puchner Do-Hyung Kim Source Type: research
Correction of symptoms of Huntington disease by genistein through FOXO3-mediated autophagy stimulation
In conclusion, treatment with genistein stimulates the autophagy process in the brains of HD mice, leading to correction of symptoms of HD, suggesting that it might be considered as a potential drug for this disease. Combined with a very recently published report indicating that impaired autophagy may be a major cause of neurodegenerative changes, these results may indicate the way to the development of effective therapeutic approaches for different neurodegenerative diseases by testing compounds (or possibly combinations of compounds) capable of stimulating autophagy and/or unblocking this process.PMID:37992314 | DOI:10.1...
Source: Autophagy - November 22, 2023 Category: Cytology Authors: Karolina Pierzynowska Magdalena Podlacha Lidia Gaffke Estera Rintz Karolina Wi śniewska Zuzanna Cyske Grzegorz W ęgrzyn Source Type: research
Autophagy activates EGR1 via MAPK/ERK to induce FGF2 in renal tubular cells for fibroblast activation and fibrosis during maladaptive kidney repair
Autophagy. 2023 Nov 18:1-22. doi: 10.1080/15548627.2023.2281156. Online ahead of print.ABSTRACTMacroautophagy/autophagy contributes to maladaptive kidney repair by inducing pro-fibrotic factors such as FGF2 (fibroblast growth factor 2), but the underlying mechanism remains elusive. Here, we show that EGR1 (early growth response 1) was induced in injured proximal tubules after ischemic acute kidney injury (AKI) and this induction was suppressed by autophagy deficiency in inducible, renal tubule-specific atg7 (autophagy related 7) knockout (iRT-atg7 KO) mice. In cultured proximal tubular cells, TGFB1 (transforming growth fac...
Source: Autophagy - November 18, 2023 Category: Cytology Authors: Man J Livingston Ming Zhang Sang-Ho Kwon Jian-Kang Chen Honglin Li Santhakumar Manicassamy Zheng Dong Source Type: research
Autophagy activates EGR1 via MAPK/ERK to induce FGF2 in renal tubular cells for fibroblast activation and fibrosis during maladaptive kidney repair
Autophagy. 2023 Nov 18:1-22. doi: 10.1080/15548627.2023.2281156. Online ahead of print.ABSTRACTMacroautophagy/autophagy contributes to maladaptive kidney repair by inducing pro-fibrotic factors such as FGF2 (fibroblast growth factor 2), but the underlying mechanism remains elusive. Here, we show that EGR1 (early growth response 1) was induced in injured proximal tubules after ischemic acute kidney injury (AKI) and this induction was suppressed by autophagy deficiency in inducible, renal tubule-specific atg7 (autophagy related 7) knockout (iRT-atg7 KO) mice. In cultured proximal tubular cells, TGFB1 (transforming growth fac...
Source: Autophagy - November 18, 2023 Category: Cytology Authors: Man J Livingston Ming Zhang Sang-Ho Kwon Jian-Kang Chen Honglin Li Santhakumar Manicassamy Zheng Dong Source Type: research
Autophagy activates EGR1 via MAPK/ERK to induce FGF2 in renal tubular cells for fibroblast activation and fibrosis during maladaptive kidney repair
Autophagy. 2023 Nov 18:1-22. doi: 10.1080/15548627.2023.2281156. Online ahead of print.ABSTRACTMacroautophagy/autophagy contributes to maladaptive kidney repair by inducing pro-fibrotic factors such as FGF2 (fibroblast growth factor 2), but the underlying mechanism remains elusive. Here, we show that EGR1 (early growth response 1) was induced in injured proximal tubules after ischemic acute kidney injury (AKI) and this induction was suppressed by autophagy deficiency in inducible, renal tubule-specific atg7 (autophagy related 7) knockout (iRT-atg7 KO) mice. In cultured proximal tubular cells, TGFB1 (transforming growth fac...
Source: Autophagy - November 18, 2023 Category: Cytology Authors: Man J Livingston Ming Zhang Sang-Ho Kwon Jian-Kang Chen Honglin Li Santhakumar Manicassamy Zheng Dong Source Type: research
Autophagy activates EGR1 via MAPK/ERK to induce FGF2 in renal tubular cells for fibroblast activation and fibrosis during maladaptive kidney repair
Autophagy. 2023 Nov 18:1-22. doi: 10.1080/15548627.2023.2281156. Online ahead of print.ABSTRACTMacroautophagy/autophagy contributes to maladaptive kidney repair by inducing pro-fibrotic factors such as FGF2 (fibroblast growth factor 2), but the underlying mechanism remains elusive. Here, we show that EGR1 (early growth response 1) was induced in injured proximal tubules after ischemic acute kidney injury (AKI) and this induction was suppressed by autophagy deficiency in inducible, renal tubule-specific atg7 (autophagy related 7) knockout (iRT-atg7 KO) mice. In cultured proximal tubular cells, TGFB1 (transforming growth fac...
Source: Autophagy - November 18, 2023 Category: Cytology Authors: Man J Livingston Ming Zhang Sang-Ho Kwon Jian-Kang Chen Honglin Li Santhakumar Manicassamy Zheng Dong Source Type: research
Transcription factor FOXM1 specifies chromatin DNA to extracellular vesicles
Autophagy. 2023 Nov 16. doi: 10.1080/15548627.2023.2284523. Online ahead of print.ABSTRACTExtracellular vesicle DNAs (evDNAs) hold significant diagnostic value for various diseases and facilitate transcellular transfer of genetic material. Our study identifies transcription factor FOXM1 as a mediator for directing chromatin genes or DNA fragments (termed FOXM1-chDNAs) to extracellular vesicles (EVs). FOXM1 binds to MAP1LC3/LC3 in the nucleus, and FOXM1-chDNAs, such as the DUX4 gene and telomere DNA, are designated by FOXM1 binding and translocated to the cytoplasm before being released to EVs through the secretory autophag...
Source: Autophagy - November 17, 2023 Category: Cytology Authors: Yunsheng Zhang Nana Ding Yizhen Li Min Ouyang Ping Fu Yousong Peng Yongjun Tan Source Type: research
Aerobic exercise attenuates autophagy-lysosomal flux deficits by ADRB2/ β2-adrenergic receptor-mediated V-ATPase assembly factor VMA21 signaling in APP-PSEN1/PS1 mice
Autophagy. 2023 Nov 15:1-17. doi: 10.1080/15548627.2023.2281134. Online ahead of print.ABSTRACTGrowing evidence suggests that macroautophagy/autophagy-lysosomal pathway deficits contribute to the accumulation of amyloid-β (Aβ) in Alzheimer disease (AD). Aerobic exercise (AE) has long been investigated as an approach to delay and treat AD, although the exact role and mechanism are not well known. Here, we revealed that AE could reverse autophagy-lysosomal deficits via activation of ADRB2/β2-adrenergic receptor, leading to significant attenuation of amyloid-β pathology in APP-PSEN1/PS1 mice. Molecular mechanism research ...
Source: Autophagy - November 15, 2023 Category: Cytology Authors: Jia-Jun Wu Haitao Yu Shu-Guang Bi Zhong-Xuan Wang Juan Gong Yu-Ming Mao Fang-Zhou Wang Yu-Qi Zhang Yun-Juan Nie Gao-Shang Chai Source Type: research
Aerobic exercise attenuates autophagy-lysosomal flux deficits by ADRB2/ β2-adrenergic receptor-mediated V-ATPase assembly factor VMA21 signaling in APP-PSEN1/PS1 mice
Autophagy. 2023 Nov 15:1-17. doi: 10.1080/15548627.2023.2281134. Online ahead of print.ABSTRACTGrowing evidence suggests that macroautophagy/autophagy-lysosomal pathway deficits contribute to the accumulation of amyloid-β (Aβ) in Alzheimer disease (AD). Aerobic exercise (AE) has long been investigated as an approach to delay and treat AD, although the exact role and mechanism are not well known. Here, we revealed that AE could reverse autophagy-lysosomal deficits via activation of ADRB2/β2-adrenergic receptor, leading to significant attenuation of amyloid-β pathology in APP-PSEN1/PS1 mice. Molecular mechanism research ...
Source: Autophagy - November 15, 2023 Category: Cytology Authors: Jia-Jun Wu Haitao Yu Shu-Guang Bi Zhong-Xuan Wang Juan Gong Yu-Ming Mao Fang-Zhou Wang Yu-Qi Zhang Yun-Juan Nie Gao-Shang Chai Source Type: research
Aerobic exercise attenuates autophagy-lysosomal flux deficits by ADRB2/ β2-adrenergic receptor-mediated V-ATPase assembly factor VMA21 signaling in APP-PSEN1/PS1 mice
Autophagy. 2023 Nov 15:1-17. doi: 10.1080/15548627.2023.2281134. Online ahead of print.ABSTRACTGrowing evidence suggests that macroautophagy/autophagy-lysosomal pathway deficits contribute to the accumulation of amyloid-β (Aβ) in Alzheimer disease (AD). Aerobic exercise (AE) has long been investigated as an approach to delay and treat AD, although the exact role and mechanism are not well known. Here, we revealed that AE could reverse autophagy-lysosomal deficits via activation of ADRB2/β2-adrenergic receptor, leading to significant attenuation of amyloid-β pathology in APP-PSEN1/PS1 mice. Molecular mechanism research ...
Source: Autophagy - November 15, 2023 Category: Cytology Authors: Jia-Jun Wu Haitao Yu Shu-Guang Bi Zhong-Xuan Wang Juan Gong Yu-Ming Mao Fang-Zhou Wang Yu-Qi Zhang Yun-Juan Nie Gao-Shang Chai Source Type: research
