MCOLN1/TRPML1 in the lysosome: a promising target for autophagy modulation in diverse diseases
Autophagy. 2024 Mar 24:1-11. doi: 10.1080/15548627.2024.2333715. Online ahead of print.ABSTRACTMCOLN1/TRPML1 is a nonselective cationic channel specifically localized to the late endosome and lysosome. With its property of mediating the release of several divalent cations such as Ca2+, Zn2+ and Fe2+ from the lysosome to the cytosol, MCOLN1 plays a pivotal role in regulating a variety of cellular events including endocytosis, exocytosis, lysosomal biogenesis, lysosome reformation, and especially in Macroautophagy/autophagy. Autophagy is a highly conserved catabolic process that maintains cytoplasmic integrity by removing su...
Source: Autophagy - March 24, 2024 Category: Cytology Authors: Jiansong Qi Qingqing Li Tianli Xin Qixia Lu Jinyi Lin Yang Zhang Haiting Luo Feifei Zhang Yanhong Xing Wuyang Wang Derong Cui Mengmeng Wang Source Type: research
Design and validation of a reporter mouse to study the dynamic regulation of TFEB and TFE3 activity through < em > in vivo < /em > imaging techniques
Autophagy. 2024 Mar 24. doi: 10.1080/15548627.2024.2334111. Online ahead of print.ABSTRACTTFEB and TFE3 belong to the MiT/TFE family of transcription factors that bind identical DNA responsive elements in the regulatory regions of target genes. They are involved in regulating lysosomal biogenesis, function, exocytosis, autophagy, and lipid catabolism. Precise control of TFEB and TFE3 activity is crucial for processes such as senescence, stress response, energy metabolism, and cellular catabolism. Dysregulation of these factors is implicated in various diseases, thus researchers have explored pharmacological approaches to m...
Source: Autophagy - March 24, 2024 Category: Cytology Authors: Electra Brunialti Nicoletta Rizzi Rita Pinto-Costa Alessandro Villa Alessia Panzeri Clara Meda Monica Rebecchi Donato A Di Monte Paolo Ciana Source Type: research
Impaired neuronal macroautophagy in the prelimbic cortex contributes to comorbid anxiety-like behaviors in rats with chronic neuropathic pain
Autophagy. 2024 Mar 24:1-18. doi: 10.1080/15548627.2024.2330038. Online ahead of print.ABSTRACTA large proportion of patients with chronic pain experience co-morbid anxiety. The medial prefrontal cortex (mPFC) is proposed to underlie this comorbidity, but the molecular and neuronal mechanisms are not fully understood. Here, we reported that impaired neuronal macroautophagy in the prelimbic cortical (PrL) subregion of the mPFC paralleled the occurrence of anxiety-like behaviors in rats with chronic spared nerve injury (SNI). Intriguingly, such macroautophagy impairment was mainly observed in a FOS/c-Fos+ neuronal subpopulat...
Source: Autophagy - March 24, 2024 Category: Cytology Authors: Su Fu Haojie Sun Jiaxin Wang Shuaixin Gao Liu Zhu Kun Cui Shimeng Liu Xuetao Qi Rui Guan Xiaocen Fan Qingying Liu Wen Chen Li Su Shuang Cui Feifei Liao Fengyu Liu Catherine C L Wong Ming Yi You Wan Source Type: research
MCOLN1/TRPML1 in the lysosome: a promising target for autophagy modulation in diverse diseases
Autophagy. 2024 Mar 24:1-11. doi: 10.1080/15548627.2024.2333715. Online ahead of print.ABSTRACTMCOLN1/TRPML1 is a nonselective cationic channel specifically localized to the late endosome and lysosome. With its property of mediating the release of several divalent cations such as Ca2+, Zn2+ and Fe2+ from the lysosome to the cytosol, MCOLN1 plays a pivotal role in regulating a variety of cellular events including endocytosis, exocytosis, lysosomal biogenesis, lysosome reformation, and especially in Macroautophagy/autophagy. Autophagy is a highly conserved catabolic process that maintains cytoplasmic integrity by removing su...
Source: Autophagy - March 24, 2024 Category: Cytology Authors: Jiansong Qi Qingqing Li Tianli Xin Qixia Lu Jinyi Lin Yang Zhang Haiting Luo Feifei Zhang Yanhong Xing Wuyang Wang Derong Cui Mengmeng Wang Source Type: research
Design and validation of a reporter mouse to study the dynamic regulation of TFEB and TFE3 activity through < em > in vivo < /em > imaging techniques
Autophagy. 2024 Mar 24. doi: 10.1080/15548627.2024.2334111. Online ahead of print.ABSTRACTTFEB and TFE3 belong to the MiT/TFE family of transcription factors that bind identical DNA responsive elements in the regulatory regions of target genes. They are involved in regulating lysosomal biogenesis, function, exocytosis, autophagy, and lipid catabolism. Precise control of TFEB and TFE3 activity is crucial for processes such as senescence, stress response, energy metabolism, and cellular catabolism. Dysregulation of these factors is implicated in various diseases, thus researchers have explored pharmacological approaches to m...
Source: Autophagy - March 24, 2024 Category: Cytology Authors: Electra Brunialti Nicoletta Rizzi Rita Pinto-Costa Alessandro Villa Alessia Panzeri Clara Meda Monica Rebecchi Donato A Di Monte Paolo Ciana Source Type: research
Foot-and-mouth disease virus VP1 degrades YTHDF2 through autophagy to regulate IRF3 activity for viral replication
In conclusion, our study revealed an underlying mechanism of how VP1 negatively regulates innate immunity through the autophagy pathway, which would contribute to understanding the negative regulation of host innate immune responses and the function of GTPBP4 and YTHDF2 during FMDV infection.Abbreviation: 3-MA:3-methyladenine; ACTB: actin beta; ATG: autophagy related; ChIP:chromatin immunoprecipitation; CQ: chloroquine; DAPI:4',6-diamidino-2-phenylindole; dpi: days post-infection; EV71:enterovirus 71; FMDV: foot-and-mouth disease virus; GTPBP4/NOG1: GTPbinding protein 4; HIF1A: hypoxia inducible factor 1 subunit alpha;hpt:...
Source: Autophagy - March 22, 2024 Category: Cytology Authors: Huisheng Liu Qiao Xue Fan Yang Weijun Cao Pengfei Liu Xiangtao Liu Zixiang Zhu Haixue Zheng Source Type: research
Foot-and-mouth disease virus VP1 degrades YTHDF2 through autophagy to regulate IRF3 activity for viral replication
In conclusion, our study revealed an underlying mechanism of how VP1 negatively regulates innate immunity through the autophagy pathway, which would contribute to understanding the negative regulation of host innate immune responses and the function of GTPBP4 and YTHDF2 during FMDV infection.Abbreviation: 3-MA:3-methyladenine; ACTB: actin beta; ATG: autophagy related; ChIP:chromatin immunoprecipitation; CQ: chloroquine; DAPI:4',6-diamidino-2-phenylindole; dpi: days post-infection; EV71:enterovirus 71; FMDV: foot-and-mouth disease virus; GTPBP4/NOG1: GTPbinding protein 4; HIF1A: hypoxia inducible factor 1 subunit alpha;hpt:...
Source: Autophagy - March 22, 2024 Category: Cytology Authors: Huisheng Liu Qiao Xue Fan Yang Weijun Cao Pengfei Liu Xiangtao Liu Zixiang Zhu Haixue Zheng Source Type: research
Foot-and-mouth disease virus VP1 degrades YTHDF2 through autophagy to regulate IRF3 activity for viral replication
In conclusion, our study revealed an underlying mechanism of how VP1 negatively regulates innate immunity through the autophagy pathway, which would contribute to understanding the negative regulation of host innate immune responses and the function of GTPBP4 and YTHDF2 during FMDV infection.Abbreviation: 3-MA:3-methyladenine; ACTB: actin beta; ATG: autophagy related; ChIP:chromatin immunoprecipitation; CQ: chloroquine; DAPI:4',6-diamidino-2-phenylindole; dpi: days post-infection; EV71:enterovirus 71; FMDV: foot-and-mouth disease virus; GTPBP4/NOG1: GTPbinding protein 4; HIF1A: hypoxia inducible factor 1 subunit alpha;hpt:...
Source: Autophagy - March 22, 2024 Category: Cytology Authors: Huisheng Liu Qiao Xue Fan Yang Weijun Cao Pengfei Liu Xiangtao Liu Zixiang Zhu Haixue Zheng Source Type: research
Correction
Autophagy. 2024 Mar 21:1-3. doi: 10.1080/15548627.2024.2317238. Online ahead of print.NO ABSTRACTPMID:38511403 | DOI:10.1080/15548627.2024.2317238 (Source: Autophagy)
Source: Autophagy - March 21, 2024 Category: Cytology Source Type: research
CSNK2/CK2 regulates selective autophagy of the endoplasmic reticulum
Autophagy. 2024 Mar 21:1-2. doi: 10.1080/15548627.2024.2330037. Online ahead of print.ABSTRACTTuning and assimilation of endoplasmic reticulum (ER) content in each cell of the human body is an essential part of organismal homeostasis and adaptation to stress. As such, the lysosomal turnover of ER (reticulophagy) needs to be regulated in a spatio-temporal as well as cell-type specific manner. We recently identified CSNK2/CK2 (casein kinase 2) as the enzyme that phosphorylates the reticulophagy receptors RETREG1/FAM134B and RETREG3/FAM134C and regulates their activity. Phosphorylation of the receptors is a prerequisite for t...
Source: Autophagy - March 21, 2024 Category: Cytology Authors: Alexandra Stolz Source Type: research
PLD1 promotes Spindle Assembly and migration through regulating autophagy in mouse oocyte meiosis
This study for the first time introduces a unique perspective on autophagic activity and function in oocyte meiotic development.PMID:38513669 | DOI:10.1080/15548627.2024.2333164 (Source: Autophagy)
Source: Autophagy - March 21, 2024 Category: Cytology Authors: Jiaqi Zhang Ying Tian Xiangning Xu Bicheng Wang Ziqi Huang Ke Song Shuo Lou Jingyi Kang Ningning Zhang Jingyu Li Jing Weng Yuanjing Liang Wei Ma Source Type: research
The activation mechanism of Atg15, a vacuolar phospholipase required for the disintegration of organelle membranes
Autophagy. 2024 Mar 21. doi: 10.1080/15548627.2024.2333165. Online ahead of print.ABSTRACTThe disintegration of cytoplasm-to-vacuole targeting (Cvt) bodies and autophagic bodies in vacuoles is essential to the Cvt pathway and macroautophagy in yeast. Atg15 is a vacuolar lipase required for the degradation of both Cvt and autophagic bodies. However, the molecular mechanism of their degradation by Atg15 remains poorly understood. In a recent study, we showed that recombinant Chaetomium thermophilum Atg15 (CtAtg15) possesses phospholipase activity, and that this activity is significantly elevated by proteolytic cleavage at a ...
Source: Autophagy - March 21, 2024 Category: Cytology Authors: Yasunori Watanabe Kuninori Suzuki Source Type: research
Correction
Autophagy. 2024 Mar 21:1-3. doi: 10.1080/15548627.2024.2317238. Online ahead of print.NO ABSTRACTPMID:38511403 | DOI:10.1080/15548627.2024.2317238 (Source: Autophagy)
Source: Autophagy - March 21, 2024 Category: Cytology Source Type: research
CSNK2/CK2 regulates selective autophagy of the endoplasmic reticulum
Autophagy. 2024 Mar 21:1-2. doi: 10.1080/15548627.2024.2330037. Online ahead of print.ABSTRACTTuning and assimilation of endoplasmic reticulum (ER) content in each cell of the human body is an essential part of organismal homeostasis and adaptation to stress. As such, the lysosomal turnover of ER (reticulophagy) needs to be regulated in a spatio-temporal as well as cell-type specific manner. We recently identified CSNK2/CK2 (casein kinase 2) as the enzyme that phosphorylates the reticulophagy receptors RETREG1/FAM134B and RETREG3/FAM134C and regulates their activity. Phosphorylation of the receptors is a prerequisite for t...
Source: Autophagy - March 21, 2024 Category: Cytology Authors: Alexandra Stolz Source Type: research
CASM mediates LRRK2 recruitment and activation under lysosomal stress
Autophagy. 2024 Mar 21:1-2. doi: 10.1080/15548627.2024.2330032. Online ahead of print.ABSTRACTConjugation of ATG8 to single membranes (CASM) at endolysosomal compartments has attracted attention as the non-autophagic function of the Atg8-family protein conjugation system, and the V-ATPase-ATG16L1 axis has emerged as a core mechanism. Our recent research has revealed that this mechanism contributes to the lysosomal recruitment and activation of LRRK2, a Parkinson disease-associated kinase that phosphorylates a subset of RAB GTPases. The activated LRRK2 under CASM-causing lysosomal stress acts to regulate lysosomal morpholog...
Source: Autophagy - March 18, 2024 Category: Cytology Authors: Tomoki Kuwahara Takeshi Iwatsubo Source Type: research
