Suppression of planktonic and biofilm of Escherichia coli by the synergistic lantibiotics-polymyxins combinations
Arch Microbiol. 2024 Mar 23;206(4):191. doi: 10.1007/s00203-024-03922-8.ABSTRACTEscherichia coli are generally resistant to the lantibiotic's action (nisin and warnerin), but we have shown increased sensitivity of E. coli to lantibiotics in the presence of subinhibitory concentrations of polymyxins. Synergistic lantibiotic-polymyxin combinations were found for polymyxins B and M. The killing of cells at the planktonic and biofilm levels was observed for two collection and four clinical multidrug-resistant E. coli strains after treatment with lantibiotic-polymyxin B combinations. Thus, 24-h treatment of E. coli mature biofi...
Source: Archives of Microbiology - March 23, 2024 Category: Microbiology Authors: Tatyana Polyudova Larisa Lemkina Daria Eroshenko Artem Esaev Source Type: research
Microbial degradation of low-density polyethylene, polyethylene terephthalate, and polystyrene by novel isolates from plastic-polluted environment
This study screened four bacterial isolates that were capable of degrading recalcitrant polymers, i.e., low-density polyethylene, polyethylene terephthalate, and polystyrene. The unique bacterial isolates were obtained from plastic polluted environment. Dermacoccus sp. MR5 (accession no. OP592184) and Corynebacterium sp. MR10 (accession no. OP536169) from Malaysian mangroves and Bacillus sp. BS5 (accession no. OP536168) and Priestia sp. TL1 (accession no. OP536170) from a sanitary landfill. The four isolates showed a gradual increase in the microbial count and the production of laccase and esterase enzymes after 4 weeks of...
Source: Archives of Microbiology - March 23, 2024 Category: Microbiology Authors: Pooja Singh Claudeen Sze Siang Lau Sze Yin Siah Kah Ooi Chua Adeline Su Yien Ting Source Type: research
Diversity, distribution, and bioprospecting potentials of carotenogenic yeast from mangrove ecosystem
This study focuses on 47 red-pigmented yeasts isolated from sediments and plant parts of 13 species of mangrove trees. The relative abundance and distribution of these yeasts varied with plant species and plant parts. The highest number of red yeasts was associated with the mangrove plant Avicennia officinalis (32%). Notably, the leaves harbored the highest percentage (45%) of carotenogenic yeasts, and definite compartmentalization of these yeast species was noticed in mangrove plant parts. All the isolates were molecularly identified and they belonged to the genera of Rhodotorula, Rhodosporidiobolus, and Cryptococcus. The...
Source: Archives of Microbiology - March 23, 2024 Category: Microbiology Authors: K A Nimsi K Manjusha M N Farzana Source Type: research
Modulating the antibacterial effect of the existing antibiotics along with repurposing drug metformin
This study suggest Metformin and other experimented drugs can be used as adjuvants boost up antibiosis but further study is needed to find out the safety and efficacy of this non-antibiotic drug as potent antibiotic adjuvant.PMID:38519821 | DOI:10.1007/s00203-024-03917-5 (Source: Archives of Microbiology)
Source: Archives of Microbiology - March 23, 2024 Category: Microbiology Authors: Md Omar Faruqe Source Type: research
Suppression of planktonic and biofilm of Escherichia coli by the synergistic lantibiotics-polymyxins combinations
Arch Microbiol. 2024 Mar 23;206(4):191. doi: 10.1007/s00203-024-03922-8.ABSTRACTEscherichia coli are generally resistant to the lantibiotic's action (nisin and warnerin), but we have shown increased sensitivity of E. coli to lantibiotics in the presence of subinhibitory concentrations of polymyxins. Synergistic lantibiotic-polymyxin combinations were found for polymyxins B and M. The killing of cells at the planktonic and biofilm levels was observed for two collection and four clinical multidrug-resistant E. coli strains after treatment with lantibiotic-polymyxin B combinations. Thus, 24-h treatment of E. coli mature biofi...
Source: Archives of Microbiology - March 23, 2024 Category: Microbiology Authors: Tatyana Polyudova Larisa Lemkina Daria Eroshenko Artem Esaev Source Type: research
Antioxidant compounds produced by endolichenic fungus Penicillium sp.-strain 1322P isolated from Pyxine subcinerea
Arch Microbiol. 2024 Mar 21;206(4):187. doi: 10.1007/s00203-024-03898-5.ABSTRACTEndolichenic fungi are expecting for new bioresources of pharmacological compounds. However, the number of investigations targeting antioxidant compounds produced by endolichenic fungi remains limited. To discover new antioxidant compounds, we analyzed the antioxidant activity of the methanol extracts derived from isolated lichen mycobionts or endolichenic fungi induced from Pyxine subcinerea. We performed this analysis using the oxygen radical absorbance capacity (ORAC) method. As a result, we isolated from an endolichenic fungus identified as...
Source: Archives of Microbiology - March 22, 2024 Category: Microbiology Authors: Hiroko Kawakami Nao Watabe Yuko Matsubuchi Kojiro Hara Masashi Komine Source Type: research
Antioxidant compounds produced by endolichenic fungus Penicillium sp.-strain 1322P isolated from Pyxine subcinerea
Arch Microbiol. 2024 Mar 21;206(4):187. doi: 10.1007/s00203-024-03898-5.ABSTRACTEndolichenic fungi are expecting for new bioresources of pharmacological compounds. However, the number of investigations targeting antioxidant compounds produced by endolichenic fungi remains limited. To discover new antioxidant compounds, we analyzed the antioxidant activity of the methanol extracts derived from isolated lichen mycobionts or endolichenic fungi induced from Pyxine subcinerea. We performed this analysis using the oxygen radical absorbance capacity (ORAC) method. As a result, we isolated from an endolichenic fungus identified as...
Source: Archives of Microbiology - March 22, 2024 Category: Microbiology Authors: Hiroko Kawakami Nao Watabe Yuko Matsubuchi Kojiro Hara Masashi Komine Source Type: research
Anti-fungal effects of novel N-(tert-butyl)-2-(pyridin-2-yl)imidazo[1,2-a]pyridin-3-amine derivative and it's in-vitro, in-silico, and mode of action against Candida spp
In this study, two newly synthesized imidazo[1,2-a]pyridine derivative (Probe I and Probe II) molecules were investigated for its antimicrobial potency against of a panel of bacterial (Gram-positive and Gram-negative bacteria) and fungal pathogens. Among the different types of pathogens, we found that Probe II showed excellent antifungal activity against fungal pathogens, based on the preliminary screening the potent molecule further investigated against multidrug-resistance Candida sp. (n = 10) and compared with commercial molecules. In addition, in-silico molecular docking, its dynamics, absorption, distribution, metabol...
Source: Archives of Microbiology - March 21, 2024 Category: Microbiology Authors: Manivannan Nandhagopal Ramanjaneyulu Mala Kanagasabai Somarathinam Divya Dhakshinamurthy Mathivanan Narayanasamy Priyadharshni Vijayan Manimuthu Mani Shankar Source Type: research
Anti-fungal effects of novel N-(tert-butyl)-2-(pyridin-2-yl)imidazo[1,2-a]pyridin-3-amine derivative and it's in-vitro, in-silico, and mode of action against Candida spp
In this study, two newly synthesized imidazo[1,2-a]pyridine derivative (Probe I and Probe II) molecules were investigated for its antimicrobial potency against of a panel of bacterial (Gram-positive and Gram-negative bacteria) and fungal pathogens. Among the different types of pathogens, we found that Probe II showed excellent antifungal activity against fungal pathogens, based on the preliminary screening the potent molecule further investigated against multidrug-resistance Candida sp. (n = 10) and compared with commercial molecules. In addition, in-silico molecular docking, its dynamics, absorption, distribution, metabol...
Source: Archives of Microbiology - March 21, 2024 Category: Microbiology Authors: Manivannan Nandhagopal Ramanjaneyulu Mala Kanagasabai Somarathinam Divya Dhakshinamurthy Mathivanan Narayanasamy Priyadharshni Vijayan Manimuthu Mani Shankar Source Type: research
Resuscitation of baijiu pit mud bacteria based on Rpf protein of Umezawaea beigongshangensis
This study lays a foundation for applying Rpf from U. beigongshangensis to exploit microbial resources in baijiu pit mud.PMID:38503937 | DOI:10.1007/s00203-024-03924-6 (Source: Archives of Microbiology)
Source: Archives of Microbiology - March 20, 2024 Category: Microbiology Authors: Hanxu Pan Jiaxuan Wang Zhanbin Sun Rui Shi Qing Ren Source Type: research
The hidden treasures in endophytic fungi: a comprehensive review on the diversity of fungal bioactive metabolites, usual analytical methodologies, and applications
Arch Microbiol. 2024 Mar 20;206(4):185. doi: 10.1007/s00203-024-03911-x.ABSTRACTThis review provides a comprehensive overview of the key aspects of the natural metabolite production by endophytic fungi, which has attracted significant attention due to its diverse biological activities and wide range of applications. Synthesized by various fungal species, these metabolites encompass compounds with therapeutic, agricultural, and commercial significance. We delved into strategies and advancements aimed at optimizing fungal metabolite production. Fungal cultivation, especially by Aspergillus, Penicillium, and Fusarium, plays a...
Source: Archives of Microbiology - March 20, 2024 Category: Microbiology Authors: Jefferson Brendon Almeida Dos Reis Adriana Sturion Lorenzi Danilo Batista Pinho Patr ícia Cardoso Cortelo Helson Mario Martins do Vale Source Type: research
An up-to-date review of biomedical applications of serratiopeptidase and its biobetter derivatives as a multi-potential metalloprotease
Arch Microbiol. 2024 Mar 19;206(4):180. doi: 10.1007/s00203-024-03889-6.ABSTRACTSerratiopeptidase is a bacterial metalloprotease used in a variety of medical applications. The multidimensional properties of serratiopeptidase make it noticeable as a miraculous enzyme. Anti-coagulant, anti-inflammatory and anti-biofilm activity of serratiopeptidase making it useful in reducing pain and swelling associated with various conditions including arthritis, diabetes, cancer, swelling, pain and also thrombolytic disorders. It breaks down fibrin, thins the fluids formed during inflammation and due to its anti-biofilm activity, can be ...
Source: Archives of Microbiology - March 19, 2024 Category: Microbiology Authors: Seyedeh Bahareh Hosseini Masoumeh Azizi Seyed Ali Nojoumi Vahideh Valizadeh Source Type: research
Unveiling growth-promoting attributes of peanut root endophyte Micromonospora sp
In this study, 20 endophytic actinobacteria were isolated from different parts of peanut plants growing in cropland with low and high salt in West Bengal, India. The endophytes underwent a rigorous morphological, biochemical, and genetic screening process to evaluate their effectiveness in enhancing plant growth. About 20% of these isolates were identified as potential plant growth-promoting endophytic actinobacteria, which showed high 16S rRNA gene sequence similarity (up to 99-100%) with different species of Micromonospora. Among these isolates, Micromonospora sp. ASENR15 produced the highest levels of indole acetic acid...
Source: Archives of Microbiology - March 19, 2024 Category: Microbiology Authors: Md Majharul Islam Sukhendu Mandal Source Type: research
Molecular cloning, expression, purification, and characterization of Bacillus subtilis hydrolyzed ginsenoside Rc of α-L-arabinofuranosidase in Escherichia coli
In this study, we successfully cloned and expressed a novel α-L-arabinofuranosidase bsafs gene (1503 bp, 501 amino acids, 55 kDa, and pI = 5.4) belonging to glycosyl hydrolase (GH) family 51 from Bacillus subtilis genome in Escherichia coli BL21 cells. The recombinant protein Bsafs was purified using Ni2+ sepharose fastflow affinity chromatography and exhibited a specific activity of 2.91 U/mg. Bsafs effectively hydrolyzed the α-L-arabinofuranoside at C20 site of ginsenoside Rc to produce Rd as the product. The Km values for hydrolysis of pNP-α-L-arabinofuranoside (pNPαAraf) and ginsenoside Rc were determined as 0.74 a...
Source: Archives of Microbiology - March 19, 2024 Category: Microbiology Authors: Ling Zhu Yuchen Wang Jian Cai Source Type: research
Performance evaluation of rhamnolipid biosurfactant produced by Pseudomonas aeruginosa and its effect on marine oil-spill remediation
This study aimed to reveal that the effect of biosurfactant on the dispersion and degradation of crude oil. Whole genome analysis showed that Pseudomonas aeruginosa GB-3 contained abundant genes involved in biosurfactant synthesis and metabolic processes and had the potential to degrade oil. The biosurfactant produced by strain GB-3 was screened by various methods. The results showed that the surface tension reduction activity was 28.6 mN·m-1 and emulsification stability was exhibited at different pH, salinity and temperature. The biosurfactant was identified as rhamnolipid by LC-MS and FTIR. The fermentation conditions o...
Source: Archives of Microbiology - March 19, 2024 Category: Microbiology Authors: Mingjun Zhu Hang Zhang Wu Cui Yuhua Su Shuo Sun Chaocheng Zhao Qiyou Liu Source Type: research
