An up-to-date review of biomedical applications of serratiopeptidase and its biobetter derivatives as a multi-potential metalloprotease
Arch Microbiol. 2024 Mar 19;206(4):180. doi: 10.1007/s00203-024-03889-6.ABSTRACTSerratiopeptidase is a bacterial metalloprotease used in a variety of medical applications. The multidimensional properties of serratiopeptidase make it noticeable as a miraculous enzyme. Anti-coagulant, anti-inflammatory and anti-biofilm activity of serratiopeptidase making it useful in reducing pain and swelling associated with various conditions including arthritis, diabetes, cancer, swelling, pain and also thrombolytic disorders. It breaks down fibrin, thins the fluids formed during inflammation and due to its anti-biofilm activity, can be ...
Source: Archives of Microbiology - March 19, 2024 Category: Microbiology Authors: Seyedeh Bahareh Hosseini Masoumeh Azizi Seyed Ali Nojoumi Vahideh Valizadeh Source Type: research
Unveiling growth-promoting attributes of peanut root endophyte Micromonospora sp
In this study, 20 endophytic actinobacteria were isolated from different parts of peanut plants growing in cropland with low and high salt in West Bengal, India. The endophytes underwent a rigorous morphological, biochemical, and genetic screening process to evaluate their effectiveness in enhancing plant growth. About 20% of these isolates were identified as potential plant growth-promoting endophytic actinobacteria, which showed high 16S rRNA gene sequence similarity (up to 99-100%) with different species of Micromonospora. Among these isolates, Micromonospora sp. ASENR15 produced the highest levels of indole acetic acid...
Source: Archives of Microbiology - March 19, 2024 Category: Microbiology Authors: Md Majharul Islam Sukhendu Mandal Source Type: research
Molecular cloning, expression, purification, and characterization of Bacillus subtilis hydrolyzed ginsenoside Rc of α-L-arabinofuranosidase in Escherichia coli
In this study, we successfully cloned and expressed a novel α-L-arabinofuranosidase bsafs gene (1503 bp, 501 amino acids, 55 kDa, and pI = 5.4) belonging to glycosyl hydrolase (GH) family 51 from Bacillus subtilis genome in Escherichia coli BL21 cells. The recombinant protein Bsafs was purified using Ni2+ sepharose fastflow affinity chromatography and exhibited a specific activity of 2.91 U/mg. Bsafs effectively hydrolyzed the α-L-arabinofuranoside at C20 site of ginsenoside Rc to produce Rd as the product. The Km values for hydrolysis of pNP-α-L-arabinofuranoside (pNPαAraf) and ginsenoside Rc were determined as 0.74 a...
Source: Archives of Microbiology - March 19, 2024 Category: Microbiology Authors: Ling Zhu Yuchen Wang Jian Cai Source Type: research
Performance evaluation of rhamnolipid biosurfactant produced by Pseudomonas aeruginosa and its effect on marine oil-spill remediation
This study aimed to reveal that the effect of biosurfactant on the dispersion and degradation of crude oil. Whole genome analysis showed that Pseudomonas aeruginosa GB-3 contained abundant genes involved in biosurfactant synthesis and metabolic processes and had the potential to degrade oil. The biosurfactant produced by strain GB-3 was screened by various methods. The results showed that the surface tension reduction activity was 28.6 mN·m-1 and emulsification stability was exhibited at different pH, salinity and temperature. The biosurfactant was identified as rhamnolipid by LC-MS and FTIR. The fermentation conditions o...
Source: Archives of Microbiology - March 19, 2024 Category: Microbiology Authors: Mingjun Zhu Hang Zhang Wu Cui Yuhua Su Shuo Sun Chaocheng Zhao Qiyou Liu Source Type: research
Important role of DNA methylation hints at significant potential in tuberculosis
Arch Microbiol. 2024 Mar 18;206(4):177. doi: 10.1007/s00203-024-03888-7.ABSTRACTTuberculosis (TB), an infectious disease caused by Mycobacterium tuberculosis (Mtb) infection, has persisted as a major global public health threat for millennia. Until now, TB continues to challenge efforts aimed at controlling it, with drug resistance and latent infections being the two main factors hindering treatment efficacy. The scientific community is still striving to understand the underlying mechanisms behind Mtb's drug resistance and latent infection. DNA methylation, a critical epigenetic modification occurring throughout an individ...
Source: Archives of Microbiology - March 18, 2024 Category: Microbiology Authors: Yuexuan Qin Tianyue Li Peiyan An Zhi Ren Jun Xi Bikui Tang Source Type: research
Extremely oligotrophic and complex-carbon-degrading microaerobic bacteria from Arabian Sea oxygen minimum zone sediments
Arch Microbiol. 2024 Mar 18;206(4):179. doi: 10.1007/s00203-024-03875-y.ABSTRACTSediments underlying marine hypoxic zones are huge sinks of unreacted complex organic matter, where despite acute O2 limitation, obligately aerobic bacteria thrive, and steady depletion of organic carbon takes place within a few meters below the seafloor. However, little knowledge exists about the sustenance and complex carbon degradation potentials of aerobic chemoorganotrophs in these sulfidic ecosystems. We isolated and characterized a number of aerobic bacterial chemoorganoheterotrophs from across a ~ 3 m sediment horizon underlying the per...
Source: Archives of Microbiology - March 18, 2024 Category: Microbiology Authors: Jagannath Sarkar Mahamadul Mondal Sabyasachi Bhattacharya Subhajit Dutta Sumit Chatterjee Nibendu Mondal Saran N Aditya Peketi Aninda Mazumdar Wriddhiman Ghosh Source Type: research
Identification and characterization of Achromobacter spanius P-9 and elucidation of its deoxynivalenol-degrading potential
Arch Microbiol. 2024 Mar 18;206(4):178. doi: 10.1007/s00203-024-03864-1.ABSTRACTDeoxynivalenol (DON) poses significant challenges due to its frequent contamination of grains and associated products. Microbial strategies for mitigating DON toxicity showed application potential. Eight bacterial isolates with DON degradation activity over 5% were obtained from various samples of organic fertilizer in this study. One of the isolates emerged as a standout, demonstrating a substantial degradation capability, achieving a 99.21% reduction in DON levels. This isolate, underwent thorough morphological, biochemical, and molecular cha...
Source: Archives of Microbiology - March 18, 2024 Category: Microbiology Authors: Feng Yao Yaowen Du Siyi Tian Guoli Chang Yanping Zhang Ruiyu Zhu Chenggang Cai Suqin Shao Ting Zhou Source Type: research
Important role of DNA methylation hints at significant potential in tuberculosis
Arch Microbiol. 2024 Mar 18;206(4):177. doi: 10.1007/s00203-024-03888-7.ABSTRACTTuberculosis (TB), an infectious disease caused by Mycobacterium tuberculosis (Mtb) infection, has persisted as a major global public health threat for millennia. Until now, TB continues to challenge efforts aimed at controlling it, with drug resistance and latent infections being the two main factors hindering treatment efficacy. The scientific community is still striving to understand the underlying mechanisms behind Mtb's drug resistance and latent infection. DNA methylation, a critical epigenetic modification occurring throughout an individ...
Source: Archives of Microbiology - March 18, 2024 Category: Microbiology Authors: Yuexuan Qin Tianyue Li Peiyan An Zhi Ren Jun Xi Bikui Tang Source Type: research
Biotransformation approach to produce rare ginsenosides F1, compound Mc1, and Rd2 from major ginsenosides
This study provides a new microbial resource for the selective conversion of protopanaxadiol-type and protopanaxatriol-type major saponins into rare ginsenosides via the whole-cell biotransformation and offers a solution for the better utilization of P. notoginseng waste.PMID:38493413 | DOI:10.1007/s00203-024-03893-w (Source: Archives of Microbiology)
Source: Archives of Microbiology - March 17, 2024 Category: Microbiology Authors: Mengxue Diao Yanchi Chen Lijun Meng Jianxiu Li Nengzhong Xie Source Type: research
Recovery and characterization of β-glucosidase-producing non-Saccharomyces yeasts from the fermentation broth of Vitis labruscana Baily × Vitis vinifera L. for investigation of their fermentation characteristics
Arch Microbiol. 2024 Mar 17;206(4):174. doi: 10.1007/s00203-024-03878-9.ABSTRACTThe present study focuses on investigating 60 strains of yeast isolated from the natural fermentation broth of Vitis labruscana Baily × Vitis vinifera L. These strains underwent screening using lysine culture medium and esculin culture medium, resulting in the identification of 27 local non-Saccharomyces yeast strains exhibiting high β-glucosidase production. Subsequent analysis of their fermentation characteristics led to the selection of four superior strains (Z-6, Z-11, Z-25, and Z-58) with excellent β-glucosidase production and fermentat...
Source: Archives of Microbiology - March 17, 2024 Category: Microbiology Authors: Ling Zhu Xuemei Zhang Yuchen Wang Xiu Gao Qingfang Xu Weiliang Liu Qihe Xu Dongmei Zhao Jian Cai Source Type: research
Isolation of phages against Streptococcus species in the oral cavity for potential control of dental diseases and associated systemic complications
This study supports the potential use of bacteriophages in controlling infections caused by Streptococcus species.PMID:38493441 | DOI:10.1007/s00203-024-03897-6 (Source: Archives of Microbiology)
Source: Archives of Microbiology - March 17, 2024 Category: Microbiology Authors: Beatrice Chepchumba George O Asudi Japhet Katana Musa O Ngayo Juliah Akhwale Khayeli Source Type: research
Proteus appendicitidis sp. nov., isolated from the appendiceal pus of an appendicitis patient in Yongzhou, China
Arch Microbiol. 2024 Mar 15;206(4):171. doi: 10.1007/s00203-024-03887-8.ABSTRACTA Gram-negative, facultatively anaerobic, short rod-shaped bacterium, designated as strain HZ0627T, was isolated from the appendiceal pus of a patient with appendicitis in Yongzhou, Hunan, China. This strain was subjected to comprehensive phenotypic, phylogenetic, and genomic analyses using polyphasic taxonomic methods. Phylogenetic analysis of the 16S rRNA gene sequence revealed that this strain belonged to the genus Proteus and the family Morganellaceae, whereas that based on the rpoB gene sequence and phylogenomic analysis demonstrated that ...
Source: Archives of Microbiology - March 16, 2024 Category: Microbiology Authors: Jing He Zhiyun Zhang Haibo Qu Gongqin Chen Junfeng Zhou Wangxi Zhou Yao Peng Xunchao Cai Source Type: research
Foliar phenols and flavonoids level in pteridophytes: an insight to culturable fungal endophyte colonisation
Arch Microbiol. 2024 Mar 15;206(4):170. doi: 10.1007/s00203-024-03880-1.ABSTRACTThere are many available reports of secondary metabolites as bioactive molecules from culturable endophytes, nevertheless, there are scarce research pertaining to the levels of metabolites in plants with respect to the incidence and colonisation of fungal endophytes in the same foliar tissues. Therefore, the study was focussed to examine whether fungal endophyte colonisation and the accumulation of secondary metabolites, such as flavonoids and phenols, in the plants are related in any way. For this reason, the study aims to analyse phenols and ...
Source: Archives of Microbiology - March 16, 2024 Category: Microbiology Authors: Royee Singha Dipashree Sharma Ajay Krishna Saha Panna Das Source Type: research
The usefulness of the MALDI-TOF MS technique in the determination of dairy samples' microbial composition: comparison of the new EXS 2600 system with MALDI Biotyper platform
This study compared the EXS 2600 system with the MALDI Biotyper for identifying microorganisms in dairy samples. Of the 196 bacterial isolates from milk, whey, buttermilk, cream, and dairy wastewater, the species and genus consistent identification between two systems showed 74% and 99%, respectively. However, the level of species identification rate exhibited a difference, which was higher in Zybio than in Bruker-76.0% and 66.8%, respectively. Notably, the EXS 2600 system performed better with certain yeast species and H. alvei, while the Biotyper excelled with Pseudomonas bacteria. Unique microbial compositions were foun...
Source: Archives of Microbiology - March 16, 2024 Category: Microbiology Authors: Gra żyna Czeszewska-Rosiak Micha ł Złoch Monika Radosi ńska Aleksandra Bogumi ła Florkiewicz Andrzej Tretyn Pawe ł Pomastowski Source Type: research
Metabolic and physiological adaptations of microalgal growth-promoting bacterium Azospirillum brasilense growing under biogas atmosphere: a microarray-based transcriptome analysis
Arch Microbiol. 2024 Mar 16;206(4):173. doi: 10.1007/s00203-024-03890-z.ABSTRACTUsing microalgal growth-promoting bacteria (MGPB) to improve the cultured microalga metabolism during biotechnological processes is one of the most promising strategies to enhance their benefits. Nonetheless, the culture condition effect used during the biotechnological process on MGPB growth and metabolism is key to ensure the expected positive bacterium growth and metabolism of microalgae. In this sense, the present research study investigated the effect of the synthetic biogas atmosphere (75% CH4-25% CO2) on metabolic and physiological adapt...
Source: Archives of Microbiology - March 16, 2024 Category: Microbiology Authors: Carolina Garciglia-Mercado Claudia A Contreras Francisco J Choix Luz E de-Bashan Gracia A G ómez-Anduro Oskar A Palacios Source Type: research
