Exploration of culturable bacterial associates of aphids and their interactions with entomopathogens
Arch Microbiol. 2024 Feb 13;206(3):96. doi: 10.1007/s00203-024-03830-x.ABSTRACTAphids shelter several bacteria that benefit them in various ways. The associates having an obligatory relationship are non-culturable, while a few of facultative associates are culturable in insect cell lines, axenic media or standard microbiology media. In the present investigation, isolation, and characterization of the culturable bacterial associates of various aphid species, viz., Rhopalosiphum maidis, Rhopalosiphum padi, Sitobion avenae, Schizaphis graminum, and Lipaphis erysimi pseudobrassicae were carried out. A total of 42 isolates were...
Source: Archives of Microbiology - February 13, 2024 Category: Microbiology Authors: M P Shireesh Kumar Alagesan Keerthana None Priya Satish Kumar Singh Dinesh Rai Aman Jaiswal Morthala Shankara Sai Reddy Source Type: research
Simultaneous removal of aliphatic and aromatic crude oil hydrocarbons by Pantoea agglomerans isolated from petroleum-contaminated soil in the west of Iran
In this study, hydrocarbon degrader bacterial strains were isolated from the highly petroleum-contaminated soils in the Dehloran oil field in the west of Iran. Out of 37 isolates, 15 can grow on M9 agar medium that contains 1.5 g L-1 of crude oil as the sole carbon source. The morphological, biochemical, and 16SrRNA sequencing analyses were performed for the isolates. The choosing of the isolates as the hydrocarbon degrader was examined by evaluating the efficacy of their crude oil removal at a concentration of 10 g L-1 in an aqueous medium. The results showed that five isolates belonging to Pseudomonas sp., Pseudomonas or...
Source: Archives of Microbiology - February 13, 2024 Category: Microbiology Authors: Saman Hosseini Rouhallah Sharifi Alireza Habibi Source Type: research
Prediction of motif-mediated viral mimicry through the integration of host-pathogen interactions
Arch Microbiol. 2024 Feb 9;206(3):94. doi: 10.1007/s00203-024-03832-9.ABSTRACTOne of the mechanisms viruses use in hijacking host cellular machinery is mimicking Short Linear Motifs (SLiMs) in host proteins to maintain their life cycle inside host cells. In the face of the escalating volume of virus-host protein-protein interactions (vhPPIs) documented in databases; the accurate prediction of molecular mimicry remains a formidable challenge due to the inherent degeneracy of SLiMs. Consequently, there is a pressing need for computational methodologies to predict new instances of viral mimicry. Our present study introduces a...
Source: Archives of Microbiology - February 9, 2024 Category: Microbiology Authors: Sobia Idrees Keshav Raj Paudel Philip M Hansbro Source Type: research
A comprehensive investigation of the medicinal efficacy of antimicrobial fusion peptides expressed in probiotic bacteria for the treatment of pan drug-resistant (PDR) infections
Arch Microbiol. 2024 Feb 8;206(3):93. doi: 10.1007/s00203-023-03823-2.ABSTRACTThe present work aimed to examine the intracellular antibacterial efficacy of Recombinant Lactobacillus acidophilus/antimicrobial peptides (AMPs) Melittin and Alyteserin-1a, specifically targeting Gram-negative bacteria. The first assessment was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Recombinant L. acidophilus/AMPs versus Gram-negative and Gram-positive bacteria. In addition, the researchers examined the in vitro viability and safety of AMPs generated by L. acidophilus. The experime...
Source: Archives of Microbiology - February 8, 2024 Category: Microbiology Authors: Maryam Safarpour-Dehkordi Omid Chabok Mohsen Asgari Reyhaneh Khademi Abbas Doosti Source Type: research
Diaporthe phaseolorum causing dieback disease on Melia dubia cav. in Karnataka state (India)
Arch Microbiol. 2024 Feb 6;206(3):92. doi: 10.1007/s00203-023-03821-4.ABSTRACTMelia dubia is an important tree species grown worldwide for its medicinal and timber values. It is widely used in timber and pulp industry and also as an organic pesticide, fertilisers, agro-forestry and herbal formulations. During 2019-2022, a dieback disease in plantations of M. dubia was recorded in Mysore, Mandya, Chamarajanagar, Hassan and Tumkur districts of Karnataka state (India) with disease incidence of 26.25%. The associated pathogen was isolated on PDA medium and its morpho-cultural characteristics were studied. The genomic DNA of th...
Source: Archives of Microbiology - February 6, 2024 Category: Microbiology Authors: V Krupalini G R Janardhana Source Type: research
Immunoinformatics design of novel multi-epitope vaccine against Trueperella Pyogenes using collagen adhesion protein, fimbriae, and pyolysin
Arch Microbiol. 2024 Feb 5;206(3):90. doi: 10.1007/s00203-023-03814-3.ABSTRACTTrueperella pyogenes (T. pyogenes) is an opportunistic pathogen that causes infertility, mastitis, and metritis in animals. T. pyogenes is also a zoonotic disease and is considered an economic loss agent in the livestock industry. Therefore, vaccine development is necessary. Using an immunoinformatics approach, this study aimed to construct a multi-epitope vaccine against T. pyogenes. The collagen adhesion protein, fimbriae, and pyolysin (PLO) sequences were initially retrieved. The HTL, CTL, and B cell epitopes were predicted. The vaccine was de...
Source: Archives of Microbiology - February 5, 2024 Category: Microbiology Authors: Babak Beikzadeh Source Type: research
Sterically hindered phenolic derivatives: effect on the production of Pseudomonas aeruginosa virulence factors, high-throughput virtual screening and ADME properties prediction
Arch Microbiol. 2024 Feb 6;206(3):91. doi: 10.1007/s00203-023-03827-y.ABSTRACTInhibition of quorum sensing is considered to be an effective strategy of control and treatment of a wide range of acute and persistent infections. Pseudomonas aeruginosa is an opportunistic bacterium with a high adaptation potential that contributes to healthcare-associated infections. In the present study, the effects of the synthesized hybrid structures bearing sterically hindered phenolic and heterocyclic moieties in a single scaffold on the production of virulence factors by P. aeruginosa were determined. It has been shown that the obtained ...
Source: Archives of Microbiology - February 5, 2024 Category: Microbiology Authors: Maxim Y Gvozdev Iveta S Turomsha Viktoryia V Savich Yaroslav V Faletrov Anastasiya V Sidarenka Vladimir M Shkumatov Natalia V Loginova Source Type: research
Biofilm formation and EPS production enhances the bioremediation potential of Pseudomonas species: a novel study from eutrophic waters of Dal lake, Kashmir, India
This study has for the first time successfully revealed the bioremediation potential of P. otitis, P. cedrina, P. azotoform, and P. peli species, thus contributing to the growing list of known nitrate-reducing Pseudomonas species. Based upon the results, these strains can be extrapolated to nitrate-polluted water systems for combating water pollution.PMID:38308703 | DOI:10.1007/s00203-023-03817-0 (Source: Archives of Microbiology)
Source: Archives of Microbiology - February 3, 2024 Category: Microbiology Authors: Riasa Zaffar Ruqeya Nazir Mushtaq Ahmad Rather Rubiya Dar Source Type: research
Natural products from Streptomyces spp. as potential inhibitors of the major factors (holoRdRp and nsp13) for SARS-CoV-2 replication: an in silico approach
This study focuses on exploring microbial natural products from soil-isolated bacteria Streptomyces sp. strain 196 and RI.24 as a potential source of new targeted drugs against SARS-CoV-2. Molecular docking studies were performed on holoRdRp and nsp13, two key factors responsible for virus replication factor. Our in silico studies, K-252-C aglycone indolocarbazole alkaloid (K252C) and daunorubicin were found to have better binding affinities than the respective control drugs, with K252C exhibiting binding energy of - 9.1 kcal/mol with holoRdRp and - 9.2 kcal/mol with nsp13, and daunorubicin showing binding energy at - 8.1 ...
Source: Archives of Microbiology - February 2, 2024 Category: Microbiology Authors: Prateek Kumar None Parveen Nafis Raj Munendra Kumar Khalid Umar Fakhri Sugandh Kumar Azmat Ali Khan Amer M Alanazi Renu Solanki None Harsha Nikhat Manzoor Monisha Khanna Kapur Source Type: research
Exposure of Legionella pneumophila to low-shear modeled microgravity: impact on stress response, membrane lipid composition, pathogenicity to macrophages and interrelated genes expression
Arch Microbiol. 2024 Feb 2;206(2):87. doi: 10.1007/s00203-023-03753-z.ABSTRACTHere, we studied the effect of low-shear modeled microgravity (LSMMG) on cross stress resistance (heat, acid, and oxidative), fatty acid content, and pathogenicity along with alteration in expression of stress-/virulence-associated genes in Legionella pneumophila. The stress resistance analysis result indicated that bacteria cultivated under LSMMG environments showed higher resistance with elevated D-values at 55 °C and in 1 mM of hydrogen peroxide (H2O2) conditions compared to normal gravity (NG)-grown bacteria. On the other hand, there was no ...
Source: Archives of Microbiology - February 2, 2024 Category: Microbiology Authors: Sunirmal Sheet Yesupatham Sathishkumar Satabdi Acharya Yang Soo Lee Source Type: research
Understanding the intricacies of microbial biofilm formation and its endurance in chronic infections: a key to advancing biofilm-targeted therapeutic strategies
Arch Microbiol. 2024 Feb 1;206(2):85. doi: 10.1007/s00203-023-03802-7.ABSTRACTBacterial biofilms can adhere to various surfaces in the environment with human beings being no exception. Enclosed in a self-secreted matrix which contains extracellular polymeric substances, biofilms are intricate communities of bacteria that play a significant role across various sectors and raise concerns for public health, medicine and industries. These complex structures allow free-floating planktonic cells to adopt multicellular mode of growth which leads to persistent infections. This is of great concern as biofilms can withstand external...
Source: Archives of Microbiology - February 1, 2024 Category: Microbiology Authors: Freeda Princy Dsouza Susha Dinesh Sameer Sharma Source Type: research
©Relationship between endophytic fungal diversity and colonization and soil factors of cultured blueberry roots in Guizhou Province, Southwest China
In this study, four cultivars (Gardenblue, Powderblue, O'Neal, and Legacy) in three areas (Gaopo, Majiang, and Fenggang) in Guizhou were used to identify DSEs by morphological and molecular biological methods and to clarify the relationship between DSE diversity and DSE colonization and soil factors of cultivated blueberries in Guizhou. The DSEs isolated from cultivated blueberry roots in 3 areas in Guizhou Province were different, belonging to 17 genera, and the dominant genera were Penicillium, Phialocephala, and Thozetella. DSEs isolated from Majiang belonged to 12 genera and 16 species, those from Gaopo belonged to 7 g...
Source: Archives of Microbiology - February 1, 2024 Category: Microbiology Authors: Si Li Xiao-Jing Shang Rui Hou Source Type: research
Assessment of the in-vitro probiotic efficacy and safety of Pediococcus pentosaceus L1 and Streptococcus thermophilus L3 isolated from Laban, a popular fermented milk product
This study assessed the probiotic efficacy and safety of LAB strains isolated from Laban, a traditional fermented milk product. Seven primarily selected Gram-positive, catalase-negative, non-spore-forming isolates were examined for their antimicrobial activity against the bacterial pathogens Bacillus cereus, Salmonella typhi, Staphylococcus aureus, and Vibrio cholera, and the fungal pathogen Candida albicans. Two isolates, identified as Pediococcus pentosaceus L1 and Streptococcus thermophilus L3, which showed antimicrobial activity against all pathogens, were further evaluated for their probiotic competence. The selected ...
Source: Archives of Microbiology - January 31, 2024 Category: Microbiology Authors: Shanta Paul Tanim Jabid Hossain Ferdausi Ali Md Elias Hossain Tasneem Chowdhury Ibrahim Khalil Faisal Jannatul Ferdouse Source Type: research
Unravelling the transcriptome response of Enterobacter sp. S-33 under varying temperature
Arch Microbiol. 2024 Jan 31;206(2):81. doi: 10.1007/s00203-023-03792-6.ABSTRACTEnterobacter genus includes the bacteria occupying every aspect of environment, however, their adaptability at varying temperature is not clear. In the present study, we analyzed the transcriptome response of Enterobacter sp. S-33 and their functional genes under various temperatures (30-45 ℃) that were expressed and accumulated in cells under temperature-stress. During a temperature shift from 37 to 45 ℃, 165 genes showed differential expression including 112 up-regulated and 53 down-regulated. In particular, heat-shock genes such as CspA, ...
Source: Archives of Microbiology - January 31, 2024 Category: Microbiology Authors: Kiran Kumari Parva Kumar Sharma Rajnish Prakash Singh Source Type: research
Antifungal activity and mechanism of oxanthromicin against Verticillium dahliae
This study, therefore, aimed at investigating the mode of action of oxanthromicin against the phytopathogen Verticillium dahliae. We found that oxanthromicin substantially suppressed spore germination and mycelial growth in V. dahliae. Further, electron microscopy and staining with propidium iodide and Rhodamine 123 indicated that oxanthromicin causes cell membrane damage and induces changes in mitochondrial membrane potential. These findings suggest that oxanthromicin exhibits its antifungal activity by damaging fungal cell membranes. This discovery could potentially facilitate the development of oxanthromicin as a biolog...
Source: Archives of Microbiology - January 31, 2024 Category: Microbiology Authors: Li-Jun Wang Feng Wen Li-Xia Li Zhan-Feng Xia Source Type: research
