Thiolase: A Versatile Biocatalyst Employing CoA-Thioester Chemistry for Making and Breaking C-C Bonds
Annu Rev Biochem. 2023 Apr 17. doi: 10.1146/annurev-biochem-052521-033746. Online ahead of print.ABSTRACTThiolases are CoA-dependent enzymes that catalyze the thiolytic cleavage of 3-ketoacyl-CoA, as well as its reverse reaction, which is the thioester-dependent Claisen condensation reaction. Thiolases are dimers or tetramers (dimers of dimers). All thiolases have two reactive cysteines: (a) a nucleophilic cysteine, which forms a covalent intermediate, and (b) an acid/base cysteine. The best characterized thiolase is the Zoogloea ramigera thiolase, which is a bacterial biosynthetic thiolase belonging to the CT-thiolase sub...
Source: Annual Review of Biochemistry - April 17, 2023 Category: Biochemistry Authors: Rajesh K Harijan Subhadra Dalwani Tiila-Riikka Kiema Rajaram Venkatesan Rik K Wierenga Source Type: research
The Proteins of mRNA Modification: Writers, Readers, and Erasers
Annu Rev Biochem. 2023 Apr 17. doi: 10.1146/annurev-biochem-052521-035330. Online ahead of print.ABSTRACTOver the past decade, mRNA modifications have emerged as important regulators of gene expression control in cells. Fueled in large part by the development of tools for detecting RNA modifications transcriptome wide, researchers have uncovered a diverse epitranscriptome that serves as an additional layer of gene regulation beyond simple RNA sequence. Here, we review the proteins that write, read, and erase these marks, with a particular focus on the most abundant internal modification, N 6-methyladenosine (m6A). We first...
Source: Annual Review of Biochemistry - April 17, 2023 Category: Biochemistry Authors: Mathieu N Flamand Matthew Tegowski Kate D Meyer Source Type: research
Thiolase: A Versatile Biocatalyst Employing CoA-Thioester Chemistry for Making and Breaking C-C Bonds
Annu Rev Biochem. 2023 Apr 17. doi: 10.1146/annurev-biochem-052521-033746. Online ahead of print.ABSTRACTThiolases are CoA-dependent enzymes that catalyze the thiolytic cleavage of 3-ketoacyl-CoA, as well as its reverse reaction, which is the thioester-dependent Claisen condensation reaction. Thiolases are dimers or tetramers (dimers of dimers). All thiolases have two reactive cysteines: (a) a nucleophilic cysteine, which forms a covalent intermediate, and (b) an acid/base cysteine. The best characterized thiolase is the Zoogloea ramigera thiolase, which is a bacterial biosynthetic thiolase belonging to the CT-thiolase sub...
Source: Annual Review of Biochemistry - April 17, 2023 Category: Biochemistry Authors: Rajesh K Harijan Subhadra Dalwani Tiila-Riikka Kiema Rajaram Venkatesan Rik K Wierenga Source Type: research
The Proteins of mRNA Modification: Writers, Readers, and Erasers
Annu Rev Biochem. 2023 Apr 17. doi: 10.1146/annurev-biochem-052521-035330. Online ahead of print.ABSTRACTOver the past decade, mRNA modifications have emerged as important regulators of gene expression control in cells. Fueled in large part by the development of tools for detecting RNA modifications transcriptome wide, researchers have uncovered a diverse epitranscriptome that serves as an additional layer of gene regulation beyond simple RNA sequence. Here, we review the proteins that write, read, and erase these marks, with a particular focus on the most abundant internal modification, N 6-methyladenosine (m6A). We first...
Source: Annual Review of Biochemistry - April 17, 2023 Category: Biochemistry Authors: Mathieu N Flamand Matthew Tegowski Kate D Meyer Source Type: research
Thiolase: A Versatile Biocatalyst Employing CoA-Thioester Chemistry for Making and Breaking C-C Bonds
Annu Rev Biochem. 2023 Apr 17. doi: 10.1146/annurev-biochem-052521-033746. Online ahead of print.ABSTRACTThiolases are CoA-dependent enzymes that catalyze the thiolytic cleavage of 3-ketoacyl-CoA, as well as its reverse reaction, which is the thioester-dependent Claisen condensation reaction. Thiolases are dimers or tetramers (dimers of dimers). All thiolases have two reactive cysteines: (a) a nucleophilic cysteine, which forms a covalent intermediate, and (b) an acid/base cysteine. The best characterized thiolase is the Zoogloea ramigera thiolase, which is a bacterial biosynthetic thiolase belonging to the CT-thiolase sub...
Source: Annual Review of Biochemistry - April 17, 2023 Category: Biochemistry Authors: Rajesh K Harijan Subhadra Dalwani Tiila-Riikka Kiema Rajaram Venkatesan Rik K Wierenga Source Type: research
The Proteins of mRNA Modification: Writers, Readers, and Erasers
Annu Rev Biochem. 2023 Apr 17. doi: 10.1146/annurev-biochem-052521-035330. Online ahead of print.ABSTRACTOver the past decade, mRNA modifications have emerged as important regulators of gene expression control in cells. Fueled in large part by the development of tools for detecting RNA modifications transcriptome wide, researchers have uncovered a diverse epitranscriptome that serves as an additional layer of gene regulation beyond simple RNA sequence. Here, we review the proteins that write, read, and erase these marks, with a particular focus on the most abundant internal modification, N 6-methyladenosine (m6A). We first...
Source: Annual Review of Biochemistry - April 17, 2023 Category: Biochemistry Authors: Mathieu N Flamand Matthew Tegowski Kate D Meyer Source Type: research
Thiolase: A Versatile Biocatalyst Employing CoA-Thioester Chemistry for Making and Breaking C-C Bonds
Annu Rev Biochem. 2023 Apr 17. doi: 10.1146/annurev-biochem-052521-033746. Online ahead of print.ABSTRACTThiolases are CoA-dependent enzymes that catalyze the thiolytic cleavage of 3-ketoacyl-CoA, as well as its reverse reaction, which is the thioester-dependent Claisen condensation reaction. Thiolases are dimers or tetramers (dimers of dimers). All thiolases have two reactive cysteines: (a) a nucleophilic cysteine, which forms a covalent intermediate, and (b) an acid/base cysteine. The best characterized thiolase is the Zoogloea ramigera thiolase, which is a bacterial biosynthetic thiolase belonging to the CT-thiolase sub...
Source: Annual Review of Biochemistry - April 17, 2023 Category: Biochemistry Authors: Rajesh K Harijan Subhadra Dalwani Tiila-Riikka Kiema Rajaram Venkatesan Rik K Wierenga Source Type: research
The Proteins of mRNA Modification: Writers, Readers, and Erasers
Annu Rev Biochem. 2023 Apr 17. doi: 10.1146/annurev-biochem-052521-035330. Online ahead of print.ABSTRACTOver the past decade, mRNA modifications have emerged as important regulators of gene expression control in cells. Fueled in large part by the development of tools for detecting RNA modifications transcriptome wide, researchers have uncovered a diverse epitranscriptome that serves as an additional layer of gene regulation beyond simple RNA sequence. Here, we review the proteins that write, read, and erase these marks, with a particular focus on the most abundant internal modification, N 6-methyladenosine (m6A). We first...
Source: Annual Review of Biochemistry - April 17, 2023 Category: Biochemistry Authors: Mathieu N Flamand Matthew Tegowski Kate D Meyer Source Type: research
Thiolase: A Versatile Biocatalyst Employing CoA-Thioester Chemistry for Making and Breaking C-C Bonds
Annu Rev Biochem. 2023 Apr 17. doi: 10.1146/annurev-biochem-052521-033746. Online ahead of print.ABSTRACTThiolases are CoA-dependent enzymes that catalyze the thiolytic cleavage of 3-ketoacyl-CoA, as well as its reverse reaction, which is the thioester-dependent Claisen condensation reaction. Thiolases are dimers or tetramers (dimers of dimers). All thiolases have two reactive cysteines: (a) a nucleophilic cysteine, which forms a covalent intermediate, and (b) an acid/base cysteine. The best characterized thiolase is the Zoogloea ramigera thiolase, which is a bacterial biosynthetic thiolase belonging to the CT-thiolase sub...
Source: Annual Review of Biochemistry - April 17, 2023 Category: Biochemistry Authors: Rajesh K Harijan Subhadra Dalwani Tiila-Riikka Kiema Rajaram Venkatesan Rik K Wierenga Source Type: research
The Proteins of mRNA Modification: Writers, Readers, and Erasers
Annu Rev Biochem. 2023 Apr 17. doi: 10.1146/annurev-biochem-052521-035330. Online ahead of print.ABSTRACTOver the past decade, mRNA modifications have emerged as important regulators of gene expression control in cells. Fueled in large part by the development of tools for detecting RNA modifications transcriptome wide, researchers have uncovered a diverse epitranscriptome that serves as an additional layer of gene regulation beyond simple RNA sequence. Here, we review the proteins that write, read, and erase these marks, with a particular focus on the most abundant internal modification, N 6-methyladenosine (m6A). We first...
Source: Annual Review of Biochemistry - April 17, 2023 Category: Biochemistry Authors: Mathieu N Flamand Matthew Tegowski Kate D Meyer Source Type: research
Thiolase: A Versatile Biocatalyst Employing CoA-Thioester Chemistry for Making and Breaking C-C Bonds
Annu Rev Biochem. 2023 Apr 17. doi: 10.1146/annurev-biochem-052521-033746. Online ahead of print.ABSTRACTThiolases are CoA-dependent enzymes that catalyze the thiolytic cleavage of 3-ketoacyl-CoA, as well as its reverse reaction, which is the thioester-dependent Claisen condensation reaction. Thiolases are dimers or tetramers (dimers of dimers). All thiolases have two reactive cysteines: (a) a nucleophilic cysteine, which forms a covalent intermediate, and (b) an acid/base cysteine. The best characterized thiolase is the Zoogloea ramigera thiolase, which is a bacterial biosynthetic thiolase belonging to the CT-thiolase sub...
Source: Annual Review of Biochemistry - April 17, 2023 Category: Biochemistry Authors: Rajesh K Harijan Subhadra Dalwani Tiila-Riikka Kiema Rajaram Venkatesan Rik K Wierenga Source Type: research
The Proteins of mRNA Modification: Writers, Readers, and Erasers
Annu Rev Biochem. 2023 Apr 17. doi: 10.1146/annurev-biochem-052521-035330. Online ahead of print.ABSTRACTOver the past decade, mRNA modifications have emerged as important regulators of gene expression control in cells. Fueled in large part by the development of tools for detecting RNA modifications transcriptome wide, researchers have uncovered a diverse epitranscriptome that serves as an additional layer of gene regulation beyond simple RNA sequence. Here, we review the proteins that write, read, and erase these marks, with a particular focus on the most abundant internal modification, N 6-methyladenosine (m6A). We first...
Source: Annual Review of Biochemistry - April 17, 2023 Category: Biochemistry Authors: Mathieu N Flamand Matthew Tegowski Kate D Meyer Source Type: research
Transcription-Coupled Nucleotide Excision Repair and the Transcriptional Response to UV-Induced DNA Damage
Annu Rev Biochem. 2023 Apr 11. doi: 10.1146/annurev-biochem-052621-091205. Online ahead of print.ABSTRACTUltraviolet (UV) irradiation and other genotoxic stresses induce bulky DNA lesions, which threaten genome stability and cell viability. Cells have evolved two main repair pathways to remove such lesions: global genome nucleotide excision repair (GG-NER) and transcription-coupled nucleotide excision repair (TC-NER). The modes by which these subpathways recognize DNA lesions are distinct, but they converge onto the same downstream steps for DNA repair. Here, we first summarize the current understanding of these repair mec...
Source: Annual Review of Biochemistry - April 11, 2023 Category: Biochemistry Authors: Nicol ás Nieto Moreno Anouk M Olthof Jesper Q Svejstrup Source Type: research
Transcription-Coupled Nucleotide Excision Repair and the Transcriptional Response to UV-Induced DNA Damage
Annu Rev Biochem. 2023 Apr 11. doi: 10.1146/annurev-biochem-052621-091205. Online ahead of print.ABSTRACTUltraviolet (UV) irradiation and other genotoxic stresses induce bulky DNA lesions, which threaten genome stability and cell viability. Cells have evolved two main repair pathways to remove such lesions: global genome nucleotide excision repair (GG-NER) and transcription-coupled nucleotide excision repair (TC-NER). The modes by which these subpathways recognize DNA lesions are distinct, but they converge onto the same downstream steps for DNA repair. Here, we first summarize the current understanding of these repair mec...
Source: Annual Review of Biochemistry - April 11, 2023 Category: Biochemistry Authors: Nicol ás Nieto Moreno Anouk M Olthof Jesper Q Svejstrup Source Type: research
Transcription-Coupled Nucleotide Excision Repair and the Transcriptional Response to UV-Induced DNA Damage
Annu Rev Biochem. 2023 Apr 11. doi: 10.1146/annurev-biochem-052621-091205. Online ahead of print.ABSTRACTUltraviolet (UV) irradiation and other genotoxic stresses induce bulky DNA lesions, which threaten genome stability and cell viability. Cells have evolved two main repair pathways to remove such lesions: global genome nucleotide excision repair (GG-NER) and transcription-coupled nucleotide excision repair (TC-NER). The modes by which these subpathways recognize DNA lesions are distinct, but they converge onto the same downstream steps for DNA repair. Here, we first summarize the current understanding of these repair mec...
Source: Annual Review of Biochemistry - April 11, 2023 Category: Biochemistry Authors: Nicol ás Nieto Moreno Anouk M Olthof Jesper Q Svejstrup Source Type: research
