Effects of maternal nutrient restriction during gestation on bovine serum microRNA abundance
The objective was to determine the effects of maternal nutrient restriction during gestation on serum microRNA (miRNA) abundance in cattle. Primiparous Angus-cross cows (n=22) were fed either control (CON; to gain 1 Kg/week) or nutrient restricted (NR; 0.55% NEm) diets based on National Research Council requirements. On day 30 of gestation, cows were blocked by body condition and randomly assigned to one of three diets: CON (n=8) days 30-190; NR (n=7) days 30-110 followed by CON days 110-190 (NR/C); or CON (n=7) days 30-110 followed by NR days 110-190 (C/NR). At 190 days of gestation, maternal serum was collected for RNA i...
Source: Animal Reproduction Science - February 24, 2024 Category: Zoology Authors: K J McCarty A N DeCarlo R E Ricks S L Pratt N M Long Source Type: research

Effect of chemical activators after intracytoplasmic sperm injection (ICSI) on embryo development in alpacas
In conclusion, alpaca oocyte activation is more efficient with ionomycin and ethanol to produce transferable embryos.PMID:38401395 | DOI:10.1016/j.anireprosci.2024.107432 (Source: Animal Reproduction Science)
Source: Animal Reproduction Science - February 24, 2024 Category: Zoology Authors: Dionet Keny Bellido-Quispe Fidel Rodolfo Mujica Lengua Mija íl Contreras Huamani J Manuel Palomino Source Type: research

Transferrin maintains the motility rate, ATP content, and DNA integrity of common carp spermatozoa during short-term storage
In this study, it was investigated the effect of purified seminal plasma transferrin (Tf) at 10 μg/ml on relevant parameters in common carp Cyprinus carpio sperm during short-term storage. We compared sperm motility and curvilinear velocity, adenosine triphosphate (ATP) content and DNA fragmentation of fresh spermatozoa to that stored for 24, 48, 72, and 144 h with or without Tf. The percentage of motile cells and the curvilinear velocity of spermatozoa in stored samples for 72 h with transferrin supplementation were greater compared to samples with no added protein. The ATP content in samples without added transferrin wa...
Source: Animal Reproduction Science - February 23, 2024 Category: Zoology Authors: Anna Shaliutina-Loginova Dmitry S Loginov Source Type: research

Transferrin maintains the motility rate, ATP content, and DNA integrity of common carp spermatozoa during short-term storage
In this study, it was investigated the effect of purified seminal plasma transferrin (Tf) at 10 μg/ml on relevant parameters in common carp Cyprinus carpio sperm during short-term storage. We compared sperm motility and curvilinear velocity, adenosine triphosphate (ATP) content and DNA fragmentation of fresh spermatozoa to that stored for 24, 48, 72, and 144 h with or without Tf. The percentage of motile cells and the curvilinear velocity of spermatozoa in stored samples for 72 h with transferrin supplementation were greater compared to samples with no added protein. The ATP content in samples without added transferrin wa...
Source: Animal Reproduction Science - February 23, 2024 Category: Zoology Authors: Anna Shaliutina-Loginova Dmitry S Loginov Source Type: research

L-Proline: A Promising Tool for Boosting Cryotolerance and Fertilizing Ability of Cryopreserved Sperm in Animals
Anim Reprod Sci. 2024 Feb 9;263:107429. doi: 10.1016/j.anireprosci.2024.107429. Online ahead of print.ABSTRACTSperm cryopreservation technology significantly contributes to the safeguarding of genetic resources, particularly for endangered species, and supports the use of artificial insemination in domestic animals. Therefore, cryopreservation can negatively affect sperm health and function leading to reduce the freezing ability and fertility potential. Therefore, it is essential to prioritize the improvement of cryotolerance in cryopreserved sperm to enhance reproductive efficiency and ensure sustainability in livestock h...
Source: Animal Reproduction Science - February 21, 2024 Category: Zoology Authors: Sameh A Abdelnour Wael A Khalil Norhan E Khalifa Fatma Mohamed Ameen Khalil Mahmoud A E Hassan Source Type: research

L-Proline: A Promising Tool for Boosting Cryotolerance and Fertilizing Ability of Cryopreserved Sperm in Animals
Anim Reprod Sci. 2024 Feb 9;263:107429. doi: 10.1016/j.anireprosci.2024.107429. Online ahead of print.ABSTRACTSperm cryopreservation technology significantly contributes to the safeguarding of genetic resources, particularly for endangered species, and supports the use of artificial insemination in domestic animals. Therefore, cryopreservation can negatively affect sperm health and function leading to reduce the freezing ability and fertility potential. Therefore, it is essential to prioritize the improvement of cryotolerance in cryopreserved sperm to enhance reproductive efficiency and ensure sustainability in livestock h...
Source: Animal Reproduction Science - February 21, 2024 Category: Zoology Authors: Sameh A Abdelnour Wael A Khalil Norhan E Khalifa Fatma Mohamed Ameen Khalil Mahmoud A E Hassan Source Type: research

L-Proline: A Promising Tool for Boosting Cryotolerance and Fertilizing Ability of Cryopreserved Sperm in Animals
Anim Reprod Sci. 2024 Feb 9;263:107429. doi: 10.1016/j.anireprosci.2024.107429. Online ahead of print.ABSTRACTSperm cryopreservation technology significantly contributes to the safeguarding of genetic resources, particularly for endangered species, and supports the use of artificial insemination in domestic animals. Therefore, cryopreservation can negatively affect sperm health and function leading to reduce the freezing ability and fertility potential. Therefore, it is essential to prioritize the improvement of cryotolerance in cryopreserved sperm to enhance reproductive efficiency and ensure sustainability in livestock h...
Source: Animal Reproduction Science - February 21, 2024 Category: Zoology Authors: Sameh A Abdelnour Wael A Khalil Norhan E Khalifa Fatma Mohamed Ameen Khalil Mahmoud A E Hassan Source Type: research

Oocyte competence and gene expression in parthenogenetic produced embryos from repeat breeder and normally fertile buffaloes (Bubalus bubalis) raised in sub-humid tropical climate
In conclusion, oocytes obtained from RB were as developmentally competent as those collected from CR females, with similar energy metabolism and in vitro development capacity. Thus, the low fertility rate of repeat breeder buffaloes, when compared to normal cyclic females, must be due to subsequent events to the blastocyst stage.PMID:38377631 | DOI:10.1016/j.anireprosci.2024.107426 (Source: Animal Reproduction Science)
Source: Animal Reproduction Science - February 20, 2024 Category: Zoology Authors: Satish Kumar Maiana Silva Chaves Anna Clara Accioly Ferreira Ana Flavia Bezerra da Silva Leda Maria Costa Pereira William Gomes Vale Sebasti ão Tavares Rolim Filho Yeda Fumie Watanabe Luciana Magalh ães Melo Vicente Jos é de Figueirêdo Freitas Source Type: research

Effect of split weaning on follicle development and oocyte quality in multiparous sows
This study determined the effect of reducing suckled litter size from 11 to 7 piglets on day 18 of lactation on the ovarian follicular environment and oocyte developmental competence at day 21 of lactation. Thirty-nine, Large White X Landrace sows (parity 3.2 ± 0.2; mean ± SEM; range 2-6) had their litter size either maintained at 11 piglets (control); or reduced to seven piglets on day 18 of lactation (split wean (SW)). Sows were slaughtered on day 21 of lactation and ovaries were collected for analysis of follicular fluid composition and in vitro blastocyst development rates. There was no effect of split weaning on fer...
Source: Animal Reproduction Science - February 18, 2024 Category: Zoology Authors: Alice C Weaver Karen L Kind Jennifer M Kelly Paul Herde William He J van Wettere Source Type: research

Maternal prepartum supplementation of protein and energy and body condition score modulated the performance of Bos indicus-influenced cow-calf pairs
Anim Reprod Sci. 2024 Feb 12;262:107433. doi: 10.1016/j.anireprosci.2024.107433. Online ahead of print.ABSTRACTRetrospective analyses were performed on a dataset of 1188 fall-calving, Brangus cow-calf pairs. Analyses 1 sorted cows according to their initial body condition score (BCS < 5 vs. ≥ 5) and whether they received (SUP) or not (NOSUP) prepartum supplementation of protein and energy. Analyses 2 sorted cows according to their calving BCS (BCS < 5 or ≥ 5) and BCS change from calving until the start of the breeding season (lost, maintained, or gained). Cows were not estrus synchronized and were assigned to nat...
Source: Animal Reproduction Science - February 18, 2024 Category: Zoology Authors: P Moriel M Vedovatto V Izquierdo E A Palmer J M B Vendramini Source Type: research

Effect of split weaning on follicle development and oocyte quality in multiparous sows
This study determined the effect of reducing suckled litter size from 11 to 7 piglets on day 18 of lactation on the ovarian follicular environment and oocyte developmental competence at day 21 of lactation. Thirty-nine, Large White X Landrace sows (parity 3.2 ± 0.2; mean ± SEM; range 2-6) had their litter size either maintained at 11 piglets (control); or reduced to seven piglets on day 18 of lactation (split wean (SW)). Sows were slaughtered on day 21 of lactation and ovaries were collected for analysis of follicular fluid composition and in vitro blastocyst development rates. There was no effect of split weaning on fer...
Source: Animal Reproduction Science - February 18, 2024 Category: Zoology Authors: Alice C Weaver Karen L Kind Jennifer M Kelly Paul Herde William He J van Wettere Source Type: research

Maternal prepartum supplementation of protein and energy and body condition score modulated the performance of Bos indicus-influenced cow-calf pairs
Anim Reprod Sci. 2024 Feb 12;262:107433. doi: 10.1016/j.anireprosci.2024.107433. Online ahead of print.ABSTRACTRetrospective analyses were performed on a dataset of 1188 fall-calving, Brangus cow-calf pairs. Analyses 1 sorted cows according to their initial body condition score (BCS < 5 vs. ≥ 5) and whether they received (SUP) or not (NOSUP) prepartum supplementation of protein and energy. Analyses 2 sorted cows according to their calving BCS (BCS < 5 or ≥ 5) and BCS change from calving until the start of the breeding season (lost, maintained, or gained). Cows were not estrus synchronized and were assigned to nat...
Source: Animal Reproduction Science - February 18, 2024 Category: Zoology Authors: P Moriel M Vedovatto V Izquierdo E A Palmer J M B Vendramini Source Type: research

Effect of split weaning on follicle development and oocyte quality in multiparous sows
This study determined the effect of reducing suckled litter size from 11 to 7 piglets on day 18 of lactation on the ovarian follicular environment and oocyte developmental competence at day 21 of lactation. Thirty-nine, Large White X Landrace sows (parity 3.2 ± 0.2; mean ± SEM; range 2-6) had their litter size either maintained at 11 piglets (control); or reduced to seven piglets on day 18 of lactation (split wean (SW)). Sows were slaughtered on day 21 of lactation and ovaries were collected for analysis of follicular fluid composition and in vitro blastocyst development rates. There was no effect of split weaning on fer...
Source: Animal Reproduction Science - February 18, 2024 Category: Zoology Authors: Alice C Weaver Karen L Kind Jennifer M Kelly Paul Herde William He J van Wettere Source Type: research

Maternal prepartum supplementation of protein and energy and body condition score modulated the performance of Bos indicus-influenced cow-calf pairs
Anim Reprod Sci. 2024 Feb 12;262:107433. doi: 10.1016/j.anireprosci.2024.107433. Online ahead of print.ABSTRACTRetrospective analyses were performed on a dataset of 1188 fall-calving, Brangus cow-calf pairs. Analyses 1 sorted cows according to their initial body condition score (BCS < 5 vs. ≥ 5) and whether they received (SUP) or not (NOSUP) prepartum supplementation of protein and energy. Analyses 2 sorted cows according to their calving BCS (BCS < 5 or ≥ 5) and BCS change from calving until the start of the breeding season (lost, maintained, or gained). Cows were not estrus synchronized and were assigned to nat...
Source: Animal Reproduction Science - February 18, 2024 Category: Zoology Authors: P Moriel M Vedovatto V Izquierdo E A Palmer J M B Vendramini Source Type: research

ProAKAP4 as a motility long-lasting marker in Catalan donkey spermatozoa
In conclusion, proAKAP4 is identified in donkey sperm, akin to its presence in other mammals. It exhibits a positive correlation with total and progressive motility, its concentration is notably affected by the presence of cryoprotectant with significant consumption observed during the initial hour following thawing. These findings contribute to our understanding of proAKAP4 dynamics in donkey sperm, providing insights that may have implications for semen preservation and reproductive technologies in equids.PMID:38367572 | DOI:10.1016/j.anireprosci.2024.107427 (Source: Animal Reproduction Science)
Source: Animal Reproduction Science - February 17, 2024 Category: Zoology Authors: Marta Dordas-Perpiny à Nicolas Sergeant Iv án Yánez-Ortiz Vincent Mevel Jaime Catal án Jean-Fran çois Bruyas Lamia Briand-Amirat Jordi Mir ó Source Type: research