Collection of epididymal semen in the tomcat (Felix catus) by stereomicroscope-aided retrograde flushing (SARF) improves sample quality
This study aimed at performing the retrograde flushing technique, for the first time, in the tomcat. In the first experiment attempts to insert a blunted needle into tomcats' ductus deferentes were made and the cannulation time and the success rate were recorded. In the second experiment, insertion of the needle into the ductus was carried out under a stereomicroscope for one testis, while the other was submitted to mincing for sperm recovery and the samples obtained from paired testes were compared. In the first study, only twenty-eight percent of the cannulation attempts were successful whereas in the second experiment, ...
Source: Animal Reproduction Science - December 19, 2023 Category: Zoology Authors: Davide Monaco Alessandra Rota Alice Carbonari Edoardo Lillo Giovanni Michele Lacalandra Annalisa Rizzo Source Type: research
Collection of epididymal semen in the tomcat (Felix catus) by stereomicroscope-aided retrograde flushing (SARF) improves sample quality
This study aimed at performing the retrograde flushing technique, for the first time, in the tomcat. In the first experiment attempts to insert a blunted needle into tomcats' ductus deferentes were made and the cannulation time and the success rate were recorded. In the second experiment, insertion of the needle into the ductus was carried out under a stereomicroscope for one testis, while the other was submitted to mincing for sperm recovery and the samples obtained from paired testes were compared. In the first study, only twenty-eight percent of the cannulation attempts were successful whereas in the second experiment, ...
Source: Animal Reproduction Science - December 19, 2023 Category: Zoology Authors: Davide Monaco Alessandra Rota Alice Carbonari Edoardo Lillo Giovanni Michele Lacalandra Annalisa Rizzo Source Type: research
Collection of epididymal semen in the tomcat (Felix catus) by stereomicroscope-aided retrograde flushing (SARF) improves sample quality
This study aimed at performing the retrograde flushing technique, for the first time, in the tomcat. In the first experiment attempts to insert a blunted needle into tomcats' ductus deferentes were made and the cannulation time and the success rate were recorded. In the second experiment, insertion of the needle into the ductus was carried out under a stereomicroscope for one testis, while the other was submitted to mincing for sperm recovery and the samples obtained from paired testes were compared. In the first study, only twenty-eight percent of the cannulation attempts were successful whereas in the second experiment, ...
Source: Animal Reproduction Science - December 19, 2023 Category: Zoology Authors: Davide Monaco Alessandra Rota Alice Carbonari Edoardo Lillo Giovanni Michele Lacalandra Annalisa Rizzo Source Type: research
Morphological and transcriptomic analyses of embryonic development of red-eared slider Trachemys scripta elegans
This study revealed the development process of red-eared slider embryo and the dynamics of the signaling pathway affecting its development, which supplemented the theory of embryo development, and provided new ideas for the molecular mechanism of turtle embryo development.PMID:38104500 | DOI:10.1016/j.anireprosci.2023.107395 (Source: Animal Reproduction Science)
Source: Animal Reproduction Science - December 17, 2023 Category: Zoology Authors: Jing Lin Miaomiao Zhang Fangbin Liang Yunfang Ni Jiani Zhang Haitao Shi Meiling Hong Li Ding Source Type: research
Morphological and transcriptomic analyses of embryonic development of red-eared slider Trachemys scripta elegans
This study revealed the development process of red-eared slider embryo and the dynamics of the signaling pathway affecting its development, which supplemented the theory of embryo development, and provided new ideas for the molecular mechanism of turtle embryo development.PMID:38104500 | DOI:10.1016/j.anireprosci.2023.107395 (Source: Animal Reproduction Science)
Source: Animal Reproduction Science - December 17, 2023 Category: Zoology Authors: Jing Lin Miaomiao Zhang Fangbin Liang Yunfang Ni Jiani Zhang Haitao Shi Meiling Hong Li Ding Source Type: research
Cooling of alpaca spermatozoa using an extender with the addition of different percentages of seminal plasma
The objective of this study was to evaluate the effect of the addition of different percentages of seminal plasma (SP) during the cooling at 5 °C of alpaca spermatozoa from vas deferens. Fifteen pools of sperm from vas deferens were evaluated and then divided into four aliquots that were diluted to a final concentration of 30 × 106 sperm/ml with either: (1) Tris with 20% egg yolk (T-EY) (control, 0% SP), (2) T-EY with 10% SP, (3) T-EY with 25% SP, and (4) T-EY with 50% SP. Samples were cooled at 5 °C and the following sperm parameters were evaluated after 24 and 48 h of storage: motility, viability, membrane function, a...
Source: Animal Reproduction Science - December 7, 2023 Category: Zoology Authors: Mariana Luc ía Bertuzzi Edita Yola Torres Manuel Guido P érez Durand Teodosio Huanca Susana Mar ía Giuliano Mar ía Ignacia Carretero Source Type: research
Cooling of alpaca spermatozoa using an extender with the addition of different percentages of seminal plasma
The objective of this study was to evaluate the effect of the addition of different percentages of seminal plasma (SP) during the cooling at 5 °C of alpaca spermatozoa from vas deferens. Fifteen pools of sperm from vas deferens were evaluated and then divided into four aliquots that were diluted to a final concentration of 30 × 106 sperm/ml with either: (1) Tris with 20% egg yolk (T-EY) (control, 0% SP), (2) T-EY with 10% SP, (3) T-EY with 25% SP, and (4) T-EY with 50% SP. Samples were cooled at 5 °C and the following sperm parameters were evaluated after 24 and 48 h of storage: motility, viability, membrane function, a...
Source: Animal Reproduction Science - December 7, 2023 Category: Zoology Authors: Mariana Luc ía Bertuzzi Edita Yola Torres Manuel Guido P érez Durand Teodosio Huanca Susana Mar ía Giuliano Mar ía Ignacia Carretero Source Type: research
Cooling of alpaca spermatozoa using an extender with the addition of different percentages of seminal plasma
The objective of this study was to evaluate the effect of the addition of different percentages of seminal plasma (SP) during the cooling at 5 °C of alpaca spermatozoa from vas deferens. Fifteen pools of sperm from vas deferens were evaluated and then divided into four aliquots that were diluted to a final concentration of 30 × 106 sperm/ml with either: (1) Tris with 20% egg yolk (T-EY) (control, 0% SP), (2) T-EY with 10% SP, (3) T-EY with 25% SP, and (4) T-EY with 50% SP. Samples were cooled at 5 °C and the following sperm parameters were evaluated after 24 and 48 h of storage: motility, viability, membrane function, a...
Source: Animal Reproduction Science - December 7, 2023 Category: Zoology Authors: Mariana Luc ía Bertuzzi Edita Yola Torres Manuel Guido P érez Durand Teodosio Huanca Susana Mar ía Giuliano Mar ía Ignacia Carretero Source Type: research
Cooling of alpaca spermatozoa using an extender with the addition of different percentages of seminal plasma
The objective of this study was to evaluate the effect of the addition of different percentages of seminal plasma (SP) during the cooling at 5 °C of alpaca spermatozoa from vas deferens. Fifteen pools of sperm from vas deferens were evaluated and then divided into four aliquots that were diluted to a final concentration of 30 × 106 sperm/ml with either: (1) Tris with 20% egg yolk (T-EY) (control, 0% SP), (2) T-EY with 10% SP, (3) T-EY with 25% SP, and (4) T-EY with 50% SP. Samples were cooled at 5 °C and the following sperm parameters were evaluated after 24 and 48 h of storage: motility, viability, membrane function, a...
Source: Animal Reproduction Science - December 7, 2023 Category: Zoology Authors: Mariana Luc ía Bertuzzi Edita Yola Torres Manuel Guido P érez Durand Teodosio Huanca Susana Mar ía Giuliano Mar ía Ignacia Carretero Source Type: research
Cooling of alpaca spermatozoa using an extender with the addition of different percentages of seminal plasma
The objective of this study was to evaluate the effect of the addition of different percentages of seminal plasma (SP) during the cooling at 5 °C of alpaca spermatozoa from vas deferens. Fifteen pools of sperm from vas deferens were evaluated and then divided into four aliquots that were diluted to a final concentration of 30 × 106 sperm/ml with either: (1) Tris with 20% egg yolk (T-EY) (control, 0% SP), (2) T-EY with 10% SP, (3) T-EY with 25% SP, and (4) T-EY with 50% SP. Samples were cooled at 5 °C and the following sperm parameters were evaluated after 24 and 48 h of storage: motility, viability, membrane function, a...
Source: Animal Reproduction Science - December 7, 2023 Category: Zoology Authors: Mariana Luc ía Bertuzzi Edita Yola Torres Manuel Guido P érez Durand Teodosio Huanca Susana Mar ía Giuliano Mar ía Ignacia Carretero Source Type: research
Cooling of alpaca spermatozoa using an extender with the addition of different percentages of seminal plasma
The objective of this study was to evaluate the effect of the addition of different percentages of seminal plasma (SP) during the cooling at 5 °C of alpaca spermatozoa from vas deferens. Fifteen pools of sperm from vas deferens were evaluated and then divided into four aliquots that were diluted to a final concentration of 30 × 106 sperm/ml with either: (1) Tris with 20% egg yolk (T-EY) (control, 0% SP), (2) T-EY with 10% SP, (3) T-EY with 25% SP, and (4) T-EY with 50% SP. Samples were cooled at 5 °C and the following sperm parameters were evaluated after 24 and 48 h of storage: motility, viability, membrane function, a...
Source: Animal Reproduction Science - December 7, 2023 Category: Zoology Authors: Mariana Luc ía Bertuzzi Edita Yola Torres Manuel Guido P érez Durand Teodosio Huanca Susana Mar ía Giuliano Mar ía Ignacia Carretero Source Type: research
Cooling of alpaca spermatozoa using an extender with the addition of different percentages of seminal plasma
The objective of this study was to evaluate the effect of the addition of different percentages of seminal plasma (SP) during the cooling at 5 °C of alpaca spermatozoa from vas deferens. Fifteen pools of sperm from vas deferens were evaluated and then divided into four aliquots that were diluted to a final concentration of 30 × 106 sperm/ml with either: (1) Tris with 20% egg yolk (T-EY) (control, 0% SP), (2) T-EY with 10% SP, (3) T-EY with 25% SP, and (4) T-EY with 50% SP. Samples were cooled at 5 °C and the following sperm parameters were evaluated after 24 and 48 h of storage: motility, viability, membrane function, a...
Source: Animal Reproduction Science - December 7, 2023 Category: Zoology Authors: Mariana Luc ía Bertuzzi Edita Yola Torres Manuel Guido P érez Durand Teodosio Huanca Susana Mar ía Giuliano Mar ía Ignacia Carretero Source Type: research
Cooling of alpaca spermatozoa using an extender with the addition of different percentages of seminal plasma
The objective of this study was to evaluate the effect of the addition of different percentages of seminal plasma (SP) during the cooling at 5 °C of alpaca spermatozoa from vas deferens. Fifteen pools of sperm from vas deferens were evaluated and then divided into four aliquots that were diluted to a final concentration of 30 × 106 sperm/ml with either: (1) Tris with 20% egg yolk (T-EY) (control, 0% SP), (2) T-EY with 10% SP, (3) T-EY with 25% SP, and (4) T-EY with 50% SP. Samples were cooled at 5 °C and the following sperm parameters were evaluated after 24 and 48 h of storage: motility, viability, membrane function, a...
Source: Animal Reproduction Science - December 7, 2023 Category: Zoology Authors: Mariana Luc ía Bertuzzi Edita Yola Torres Manuel Guido P érez Durand Teodosio Huanca Susana Mar ía Giuliano Mar ía Ignacia Carretero Source Type: research
Additional glial cell line-derived neurotrophic factor in vitro promotes the proliferation of undifferentiated spermatogonia from sterile cattleyak
In this report, we studied the differential expression of GDNF in the testis of yak and cattleyak, and discussed the optimal concentration of GDNF in the culture medium of undifferentiated spermatogonia (UDSPG) of cattleyak in vitro and the effect of GDNF on the proliferation of cattleyak UDSPG. The results indicated that GDNF expression in the testicular tissue of cattleyak was inferior to that of yak. Moreover, the optimum value for the UDSPG in vitro culture was determined to be 20-30 ng/mL for cattleyak. In vitro, the proliferation activity of UDSPG was observed to increase with additional GDNF due to the up-regulation...
Source: Animal Reproduction Science - December 6, 2023 Category: Zoology Authors: Peng Zhang Kemin Jing Yuan Tian Yuqian Li Zhixin Chai Xin Cai Source Type: research
Selection of spermatozoa with high motility and quality from bovine frozen-thawed semen using the centrifuge-free device
In conclusion, the MIGLIS method has the potential to select functional, high-quality frozen-thawed bovine spermatozoa.PMID:38056176 | DOI:10.1016/j.anireprosci.2023.107386 (Source: Animal Reproduction Science)
Source: Animal Reproduction Science - December 6, 2023 Category: Zoology Authors: Suong T Nguyen Ayane Edo Megumi Nagahara Takeshige Otoi Masayasu Taniguchi Mitsuhiro Takagi Source Type: research
