Metabonomics Study on the hepatoprotective effect mechanism of polysaccharides from different processed products of Angelica Sinensis on the layer chickens based on UPLC-Q/TOF-MS/MS, multivariate statistical analysis and conjoint analysis

Biomed Chromatogr. 2022 Apr 7:e5362. doi: 10.1002/bmc.5362. Online ahead of print.ABSTRACTChicken Colibacillosis (CC) is one of the most severe diseases in the chicken industry. Ceftiofur sodium (CS) is often used to treat CC in clinical practice, and lipopolysaccharide (LPS) accumulates in the chicken body. Previous experimental studies found that CS combined with LPS could induce liver injury in layer chickens, and polysaccharides from charred Angelica Sinensis (CASP) had a better hepatoprotective effect than polysaccharides from unprocessed Angelica Sinensis (UASP). However, the intervention mechanism was unclear. Thus, UPLC-Q/TOF-MS/MS-based metabonomics and transcriptomics were used in this study to clarify the hepatoprotective effect mechanism of CASP and UASP on the layer chicken. Transcriptomics and enzyme-linked immunosorbent assay (ELISA) was used to make the biological verification on some critical mutual metabolic pathways screened with metabonomics. The comprehensive analysis results showed that after the layer chicken liver injury model built with LPS and CS, twelve critical metabolic pathways were disturbed, involving ten important differential metabolites. The hepatoprotective effect mechanism of CASP is related to the arachidonic acid metabolism pathway and mTOR signaling pathway, involving nine important differential metabolites. By contrast, the hepatoprotective effect mechanism of UASP is related to the arachidonic acid metabolism pathway, involving four i...
Source: Biomedical Chromatography : BMC - Category: Biomedical Science Authors: Source Type: research