Anticancer siRNA cocktails as a novel tool to treat cancer cells. Part (A). Mechanisms of interaction

Publication date: 15 May 2015 Source:International Journal of Pharmaceutics, Volume 485, Issues 1–2 Author(s): Maksim Ionov , Joanna Lazniewska , Volha Dzmitruk , Inessa Halets , Svetlana Loznikova , Darya Novopashina , Evgeny Apartsin , Olga Krasheninina , Alya Venyaminova , Katarzyna Milowska , Olga Nowacka , Rafael Gomez-Ramirez , Francisco Javier de la Mata , Jean-Pierre Majoral , Dzmitry Shcharbin , Maria Bryszewska This paper examines a perspective on the use of newly engineered nanomaterials as effective and safe carriers of genes for the therapy of cancer. Three different groups of cationic dendrimers (PAMAM, phosphorus and carbosilane) were complexed with anticancer siRNA and their biophysical properties of the dendriplexes analyzed. The potential of the dendrimers as nanocarriers for anticancer siBcl-xl, siBcl-2, siMcl-1 siRNAs and a siScrambled sequence was explored. Dendrimer/siRNA complexes were characterized by methods including fluorescence, zeta potential, dynamic light scattering, circular dichroism, gel electrophoresis and transmission electron microscopy. Some of the experiments were done with heparin to check if siRNA can be easily disassociated from the complexes, and whether released siRNA maintains its structure after interaction with the dendrimer. The results indicate that siRNAs form complexes with all the dendrimers tested. Oligoribonucleotide duplexes can be released from dendriplexes after heparin treatment and the structure ...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research