zMADM (zebrafish mosaic analysis with double markers) for single-cell gene knockout and dual-lineage tracing [Genetics]

As a vertebrate model organism, zebrafish has many unique advantages in developmental studies, regenerative biology, and disease modeling. However, tissue-specific gene knockout in zebrafish is challenging due to technical difficulties in making floxed alleles. Even when successful, tissue-level knockout can affect too many cells, making it difficult to distinguish cell...
Source: Proceedings of the National Academy of Sciences - Category: Science Authors: Tags: Genetics Biological Sciences Source Type: research