Exercise increases the expression of glucose transport and lipid metabolism genes at optimum level time point 6  h post-exercise in rat skeletal muscle

In this study, we accessed NRF-1 and its target gene expression crucial in glucose transport and lipid oxidation during exercise. Five- to 6-week-old male Wistar rats were exercised to identify the time-point for an optimum increase in the levels of NRF-1 and target genes. Gastrocnemius muscles were harvested after 0, 2, 4, 6, 8, 10, 12, and 15  h post-exercise and non-exercise rats. Primers were used to amplify the region of the genes; Nrf-1, glut 4, carnitine palmitoyltransferase, peroxisome proliferator-activated receptor gamma co-activator 1, mef2a, and acetyl-CoA carboxylase-1. Relative mRNA expression was normalized to the Actin ref erence gene. Cpt-1, Nrf-1, mef2a, glut4, cpt2, and Pgc-1 showed 2.5, 8, 1.2, 4.1, 4.6, 3.5-folds increase respectively after 8 h post-exercise compared with control, whereas Acc-1 showed a 3.1-fold decrease in gene expression ratio after 6 h post-exercise. Nrf-1 binding to cpt-1 and mef2a increase d with 3 and 3.5-folds, respectively. Nrf-1 was increased by exercise with its binding to target genes which has huge implications in ameliorating type 2 diabetes and insulin resistance.Graphical abstractExercise increases NRF-1 bound Mef2a to optimum level at time point 4  h post exercise.
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research