Megakaryocyte –stromal cell interactions: Effect on megakaryocyte proliferation, proplatelet production, and survival
Bone marrow stromal cells provide a proper environment for the development of hematologic lineages. The incorporation of different stromal cells into in vitro culture systems would be an attractive model to study megakaryopoiesis and thrombopoiesis. Our objective was to evaluate the participation of different types of stromal cells in in vitro megakaryopoiesis, thrombopoiesis, and megakaryocyte (MK) survival. CD34-positive progenitors from umbilical cord blood were differentiated into MK precursors and the n cocultured with umbilical vein endothelial cells (HUVECs), bone marrow mesenchymal stem cells (MSCs), skin fibroblasts (SFs) (all human), or the mouse fibroblast cell line L929.
Source: Experimental Hematology - Category: Hematology Authors: Nora P. Goette, Francisco R. Borzone, Ailen D. Discianni Lupi, Norma A. Chasseing, Mar ía F. Rubio, Mónica A. Costas, Paula G. Heller, Rosana F. Marta, Paola R. Lev Tags: Regular Submission Source Type: research