Development of rapid gold nanoparticles based lateral flow assays for simultaneous detection of Shigella and Salmonella genera

Lateral flow assay for simultaneous detection ofShigella genera andSalmonella genera in spiked milk sample. AbstractSalmonella andShigella genera are common pathogens that contaminate foods and beverages. Lateral flow assays (LFA) are commonly used to detect these pathogens. However, most of the developed LFAs are for single detection. Simultaneous detection of pathogens is required to reduce cost and time. In this work, 40 nm gold nanoparticles (AuNPs) were synthesized using the seeding growth method as labeling agent. The AuNPs were characterized and conjugated with mouse anti-Gram negative endotoxin antibody. The nitrocellulose membrane HF135 was immobilized with anti-mouse IgG antibody as a control line and two separate test lines with either anti-Shigella or anti-Salmonella antibody, respectively. Color intensity of test lines was observed for positive samples. A milk sample was used as proof of concept to mimic actual contamination. The limit of detection of the LFA was 3.0 × 106 CFU/mL for multiplex detection ofShigella flexneri andSalmonella Typhi and for both single detections. The result was comparable with the enzyme-linked immunosorbent assay (ELISA) analysis. The produced LFA could differentiate betweenShigella flexneri,Shigella boydii,Salmonella Enteritidis, andSalmonella Typhi. The developed LFA was able to identifyShigella flexneri andSalmonella Typhi with good sensitivity in milk samples, thus, beneficial to ensure the safety of food before entering the mark...
Source: Biotechnology and Applied Biochemistry - Category: Biochemistry Authors: Tags: Original Article Source Type: research