DNA-Binding Properties of YbaB, a Putative Nucleoid-Associated Protein From Caulobacter crescentus

In this study, we characterized the YbaB/EbfC family DNA-binding protein in Caulobacter crescentus. C. crescentus has one YbaB/EbfC family gene annotated in the genome (YbaBCc) and it shares 41% sequence identity with YbaB/EbfC family NAPs. Computational modeling revealed tweezer-like structure of YbaBCc, a characteristic of YbaB/EbfC family of NAPs. N-terminal–CFP tagged YbaBCc localized with the nucleoid and is able to compact DNA. Unlike B. burgdorferi EbfC protein, YbaBCc protein is a non-specific DNA-binding protein in C. crescentus. Moreover, YbaBCc shields DNA against enzymatic degradation. Collectively, our findings reveal that YbaBCc is a small histone-like protein and may play a role in bacterial chromosome structuring and gene regulation in C. crescentus.
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
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Source: Genes - Category: Genetics & Stem Cells Authors: Tags: Article Source Type: research
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Source: Bulletin of Mathematical Biology - Category: Bioinformatics Authors: Source Type: research
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Source: Vaccine - Category: Allergy & Immunology Authors: Source Type: research
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Source: Applied Biochemistry and Biotechnology - Category: Biochemistry Authors: Source Type: research
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Source: Molekuliarnaia Biologiia - Category: Molecular Biology Authors: Source Type: research
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Source: Best Practice and Research Clinical Haematology - Category: Hematology Source Type: research
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