Bimolecular fluorescence complementation assay to explore protein-protein interactions of the Yersinia virulence factor YopM

In this study, we aimed to explore protein-protein interactions of YopM with the two components of T3SS, namely LcrV and LcrG. We used bimolecular fluorescence complementation (BiFC) assay and monitored the reassembly of green fluorescence protein in Escherichia coli. As an indicator of the protein-protein interaction, we monitored the in vivo reconstitution of fluorescence by measuring fluorescence intensity and imaging the cells under fluorescence microscope. We showed, for the first time, that YopM interacts with LcrG, but not with LcrV. Here, we propose BiFC assay as a simple method to screen novel interaction partners of YopM.PMID:34689104 | DOI:10.1016/j.bbrc.2021.10.039
Source: Cell Research - Category: Cytology Authors: Source Type: research