Dna double-strand breaks frequently cause chromosome loss in human embryos

Genome editing by DNA double-strand breaks (DSB) is currently being investigated as a tool to treat or even prevent heritable diseases. However, DNA repair mechanisms in the human embryo remain poorly understood and may result in small genetic variations such as indels, as well as large unwanted changes including loss of heterozygosity or the loss of a whole chromosome. Our study aims to determine outcomes of DNA repair after pericentromeric Cas9 cleavage in human preimplantation embryos.
Source: Fertility and Sterility - Category: Reproduction Medicine Authors: Source Type: research