High-performance thin-layer chromatography based approach for bioassay and ATR-FTIR spectroscopy for the evaluation of antioxidant compounds from Asparagus racemosus Willd. aerial parts

In this study, arial parts of A. racemosus were investigated for total phenolic content, total flavonoid content, antioxidative potential. A high-performance thin-layer chromatography (HPTLC) method combined with effect-directed-analysis (EDA) was also developed to screen the antioxidant effects of A. racemosus and quantify biological active compounds on chromatogram from A. racemosus. Total phenolic (154 mg GAE/gm), flavonoids content (497 mg QR/gm) and IC50 (15.25 μg/ml) were found high in methanolic extract of A. racemosus than n-hexane, chloroform and ethyl acetate extract. HPTLC hyphenated with chemical derivatizations (DPPH•, p-anisaldehyde/sulfuric acid, and ferric chloride) was used to evaluate antioxidant activity, presence of phytosterols, terpenoids, and polyphenolic content. The same compounds at hRf = 58, hRf = 0.68, hRf = 74 and hRf =65 in extracts were responsible for antioxidant activity. Hyphenated HPTLC allowed a fast characterization of the active compound with the combination of EDA and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. Spectral analysis of band from ATR was identified myricetin, quercetin, p-coumaric acid, and caffeic acid to be responsible for the antioxidant activity.PMID:34407236 | DOI:10.1002/bmc.5230
Source: Biomedical Chromatography : BMC - Category: Biomedical Science Authors: Source Type: research