P –158 Assisted Hatching on D + 3 in order to facilitate trophectoderm biopsy in blastocyst for PGT-A is not advisable in all patients

AbstractStudy questionIs it useful or beneficial to perform Assisted Hatching (AH) on D  + 3 previously to biopsy for PGT-A on blastocyst stage on D + 5?Summary answerThe routine use of AH on D  + 3 to facilitate the embryo biopsy on D + 5 could negatively influence the development of the embryos to blastocyst stage.What is known alreadyThe blastocyst stage is the optimal stage for performing biopsies for PGT-A, which has been reported as a key factor determining the growing clinical application of this strategy worldwide. For trophectoderm (TE) biopsy, laser-assisted drilling is used to create a zona opening on D  + 3 or D + 5 of development. The method of zona opening on D + 3 allows some of the TE cells to herniate during blastocyst formation and expansion, which facilitates the biopsy process. However, this method may result in herniation of inner cell mass cells instead of TE or maybe could a ffect the development of the embryo to blastocyst stage.Study design, size, durationA total of 100 PGT-A cycles were performed in 2019 and 2020. In 78 of them laser-assisted drilling was used to create a zona opening on D  + 5 only in those embryos which arrived to blastocyst stage for TE biopsy (Group No-AH). In 22 cycles the same drilling was achieved on D + 3 in all embryos, independently of their quality (Group AH). The average of embryos per cycle in each group was 5 and 4.3 respectively.Participants/materials, setting, methodsA total of 100 ...
Source: Human Reproduction - Category: Reproduction Medicine Source Type: research