P –150 Does trophectoderm biopsy performed on different blastocyst stages affect the clinical outcome?

The objective of this study is to evaluate the effect of trophectoderm (TE) biopsy on different blastocyst stages and its clinical outcome.Summary answerOur results showed that TE biopsy significantly reduced the clinical outcome of fully hatched blastocyst. What is known already: TE biopsy is a method widely practiced to harvest cells to determine the chromosomal constitution of a blastocyst, ensuring higher implantation and healthy pregnancies. The effect on clinical outcome after transferring blastocysts biopsied at different blastocysts stages has not been extensively studied.Study design, size, durationThis retrospective study was conducted from January 2017 until July 2019 at Alpha IVF& Women ’s Specialists. Following laser assisted hatching on day 3, TE biopsy was performed on unhatched, hatching and fully hatched day–5 blastocysts. A total of 1,020 single euploid blastocysts transfer (SBT) were performed. The average maternal age was 31.7. Implantation rates (IR) were evaluated for all stages of hatching (Unhatched: BG3& 4; hatching: BG5; fully hatched: BG6).Participants/materials, setting, methodsLaser assisted hatching (Hamilton Thorne Bioscience, USA) was performed on day –3 and subsequently cultured to blastocyst-stage. Different hatching stages were observed using embryoscope time-lapse system (Vitrolife, Sweden) and were recorded. Day–5 blastocysts with at least BG3BB grade (Gardner’s System) were selected for TE biopsy and the biopsied cells w...
Source: Human Reproduction - Category: Reproduction Medicine Source Type: research