P –170 The secretomy of embryo sex

AbstractStudy questionDoes the analysis of the metabolites of the embryonic culture medium can predict the sex of the embryo?Summary answerThe presence and quantity of some metabolites in the culture medium can predict the sex of the human embryos.What is known alreadyAdvances in analytical techniques for metabolomics have brought the possibility of better tools for the characterization of molecules. Embryonic metabolism can be used as a good indicator of viability, regardless of the morphology of the blastocysts, since differences were observed in the metabolic activities between the days of embryo development and in the rates of live births.Study design, size, duration16 patients had their embryos biopsied between the months of January to July 2019 in a human reproduction laboratory. All cases had PGT-A indication and after the biopsy, the embryos were frozen. The culture medium samples were individually prepared for metabolites extraction according to the Bligh and Dyer protocol. Controlled ovarian stimulation and dose adjustments according to the response of each patient. The metabolomics analysis was performed by mass spectrometry.Participants/materials, setting, methodsFollicular puncture were performed 35  hours after r-hCG. The eggs were kept in individual culture until the blastocyst stage. The blastocysts biopsy was performed (20). After the culture medium was sent to the 337 metabolites analysis by mass spectrometry. 14 molecules with the highest score on the PLS...
Source: Human Reproduction - Category: Reproduction Medicine Source Type: research