Amplification by tramadol of PGD2-induced osteoprotegerin synthesis in osteoblasts: involvement of μ-opioid receptor and 5-HT transporter

Tramadol enhanced the PGD2-induced release and mRNA expression of osteoprotegerin in osteoblast-like MC3T3-E1 cells. Naloxone reduced the amplification by tramadol of the PGD2-stimulated osteoprotegerin release. Not reboxetine but fluvoxamine and sertraline upregulated the PGD2-induced osteoprotegerin release, which was further amplified by morphine. Tramadol enhanced PGD2-stimulated phosphorylation of p38 MAP kinase and SAPK/JNK, but not p44/p42 MAP kinase. Both SB203580 and SP600125 suppressed the tramadol effect to enhance the PGD2-stimulated osteoprotegerin release.
Source: Prostaglandins, Leukotrienes and Essential Fatty Acids - Category: Biomedical Science Authors: Tags: Research article Source Type: research