Characteristics of gut virome and microbiome in patients with stroke

Nan Fang Yi Ke Da Xue Xue Bao. 2021 Jun 20;41(6):862-869. doi: 10.12122/j.issn.1673-4254.2021.06.08.ABSTRACTOBJECTIVE: To explore the differences in gut virome and microbiome between patients with stroke and healthy volunteers.OBJECTIVE: Fifteen patients with acute ischemic stroke treated in the Department of Neurology of Nanfang Hospital between February, 2014 and February, 2016 and 15 healthy volunteers matched for age and sex were enrolled in this study. Virome sequencing and 16S rRNA sequencing were performed on stool samples of all the participants, and the composition and structures of the virome and microbiome were compared between the two groups.OBJECTIVE: No significant difference was found in the overall diversity of virome between the stroke patients and the healthy volunteers (alpha diversity: P=0.320; beta diversity: P=0.169, R2=0.037), but virome composition differed significantly between the two groups. The relative abundance of Bacteroides phage B40_8 and Cronobacter phage CS01 increased significantly in patients with stroke. The structures and composition of the microbiome in patients with stroke also differed significantly from those of the healthy volunteers (alpha diversity: P=0.950; beta diversity: P=0.005, R2=0.117). The relative abundance of Megasphaera increased while that of Bifidobacterium decreased in patients with stroke. Correlation analysis showed that in the virome of stroke patients, the relative abundance of the phage preying Streptococcus was...
Source: Journal of Southern Medical University - Category: Universities & Medical Training Authors: Source Type: research