Metabolic fingerprinting for discrimination of DNA-authenticated Atractylodes plants using 1H NMR spectroscopy

AbstractIdentifying different species of the genusAtractylodes which are commonly used in Chinese and Japanese traditional medicine, using chromatographic approaches can be difficult.1H NMR metabolic profiling of DNA-authenticated, archived rhizomes of the genusAtractylodes was performed for genetic and chemical evaluation. The ITS region of the nuclear rDNA was sequenced for five species,A. japonica,A. macrocephala,A. lancea,A. chinensis, andA. koreana. Our samples had nucleotide sequences as previously reported, except that part of theA. lancea cultivated in Japan had a type 5, hybrid DNA sequence. Principal component analysis (PCA) using1H NMR spectra of extracts with two solvent systems (CD3OD, CDCl3) was performed. When CDCl3 extracts were utilized, the chemometric analysis enabled the identification and classification ofAtractylodes species according to their composition of major sesquiterpene compounds. The1H NMR spectra using CD3OD contained confounding sugar peaks. PCA removal of these peaks gave the same result as that obtained using CDCl3 and allowed species distinction. Such chemometric methods with multivariate analysis of NMR spectra will be useful for the discrimination of plant species, without specifying the index components and quantitative analysis on multi-components.Graphic abstract

Source: Journal of Natural Medicines - May 28, 2021 Category: Drugs & Pharmacology Source Type: research