In vitro studies of Annona muricata L. extract ‐loaded electrospun scaffolds for localized treatment of breast cancer

AbstractThis paper presents in vitro studies of the sustained release ofAnnona muricata leaf extracts (AME) from hybrid electrospun fibers for breast cancer treatment. Electrospun hybrid scaffolds were fabricated from crude AME extracts, poly(lactic ‐co‐glycolic acid)/gelatin (PLGA/Ge) and pluronic F127. The physicochemical properties of the AME extract and scaffolds were studied. The antiproliferative effects of the scaffolds were also assessed on breast cancer (MCF‐7 and MDA‐MB‐231) and non‐tumorigenic breast (MCF10A) cell lines. Scanning electron microscope micrographs revealed a random network of micro‐ and submicron fibers. In vitro drug release profiles, governed by quasi‐Fickian diffusion at pH 7.4 and non‐Fickian super case II at pH 6.7, showed initial burst AME release from the PLGA/Ge‐AME and PLGA/Ge‐F127 /AME fibers at pH 7.4, and burst release from PLGA/Ge‐F127/AME (not observed from PLGA/Ge‐AME) at pH 6.7. Then, a slower, sustained release of the remaining AME from the fibers, attributed to the onset of degradation of the PLGA/Ge backbone, was observed for the next 72 hr. The cumulative re lease of AME was 89.33 ± 0.73% (PLGA/Ge‐AME) and 51.17 ± 7.96% (PLGA/Ge‐F127/AME) at pH 7.4, and 9.27 ± 2.3% and 73.5 ± 4.5%, respectively, at pH 6.7. Pluronic F127 addition increased the drug loading capacity and prolonged the sustained AME release from the fibers. The rel eased AME significantly inhibited the in vitro ...
Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - Category: Materials Science Authors: Tags: RESEARCH ARTICLE Source Type: research