Biochemical features and virulence gene profiles of non-O157/O26 enterohemorrhagic Escherichia coli strains from humans in Yamaguchi Prefecture, Japan.

Biochemical features and virulence gene profiles of non-O157/O26 enterohemorrhagic Escherichia coli strains from humans in Yamaguchi Prefecture, Japan. Jpn J Infect Dis. 2015 Jan 20; Authors: Kameyama M, Yabata J, Nomura Y, Tominaga K Abstract The biochemical features and virulence gene profiles of 37 enterohemorrhagic Escherichia coli (EHEC) strains belonging to serogroups other than O157 and O26 (non-O157/O26 EHEC) were investigated. All strains were isolated from humans between 2002 and 2013 in Yamaguchi Prefecture. Serogroup O111 strains were most common, followed by O103, O121, and O145. Most strains (84%) were negative for sorbose fermentation, whereas only one and two were negative for sorbitol and rhamnose fermentation, respectively. Two strains lacked β-D-glucuronidase activity. stx subtyping revealed six genotypes:stx1a (n=20), stx1a + stx2a (n=8), stx2a (n=4), stx2b (n=3), stx2a + stx2c (n=1), and stx2a + stx2d (n=1). Polymerase chain reaction screening of other toxin and adherence genes showed that astA, subA, and cdtB were present in five, two, and two strains, respectively. The intimin gene, eae, was present in 30 strains (81%). Of the seven eae-negative strains, saa and eibG were found in three and two strains, respectively; no adherence factors were detected in the remaining two strains. The antimicrobial susceptibility profiles of the strains to 12 drugs were examined and 11 strains (30%) showed resistant to one or ...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research