Anti-quorum sensing and antibiofilm potential of 1,8-cineole derived from Musa paradisiaca against Pseudomonas aeruginosa strain PAO1

This study aimed to evaluate the anti-quorum sensing and antibiofilm inhibitory potential ofMusa paradisiaca againstChromobacterium violaceum (ATCC 12472) andPseudomonas aeruginosa. The methanol extract ofM. paradisiacsa exhibits that better antibiofilm potential againstP. aeruginosa. Then, the crude methanol extract was subjected to purify by column chromatography and collected the fractions. The mass-spectrometric analysis of a methanol extract ofM. paradisiaca revealed that 1,8-cineole is the major compounds. 1, 8-cineole significantly inhibited the QS regulated violacein production inC. violaceum. Moreover, 1,8-cineole significantly inhibited the QS mediated virulence production and biofilm formation ofP. aeruginosa without affecting their growth. The real-time PCR analysis showed the downregulation of autoinducer synthase and transcriptional regulator genes upon 1,8-cineole treatment. The findings of the present study strongly suggested that metabolite ofM. paradisiaca impedesP. aeruginosa QS system and associated virulence productions.
Source: World Journal of Microbiology and Biotechnology - Category: Microbiology Source Type: research