Molecules, Vol. 26, Pages 1713: Development of New Targeted Inulin Complex Nanoaggregates for siRNA Delivery in Antitumor Therapy

Molecules, Vol. 26, Pages 1713: Development of New Targeted Inulin Complex Nanoaggregates for siRNA Delivery in Antitumor Therapy Molecules doi: 10.3390/molecules26061713 Authors: Gennara Cavallaro Carla Sardo Emanuela Fabiola Craparo Gaetano Giammona Here, a novel strategy of formulating efficient polymeric carriers based on the already described INU-IMI-DETA for gene material whose structural, functional, and biological properties can be modulated and improved was successfully investigated. In particular, two novel derivatives of INU-IMI-DETA graft copolymer were synthesized by chemical functionalisation with epidermal growth factor (EGF) or polyethylenglycol (PEG), named INU-IMI-DETA-EGF and INU-IMI-DETA-PEG, respectively, in order to improve the performance of already described “inulin complex nanoaggregates” (ICONs). The latter were thus prepared by appropriately mixing the two copolymers, by varying each component from 0 to 100 wt% on the total mixture, named EP-ICONs. It was seen that the ability of the INU-IMI-DETA-EGF/INU-IMI-DETA-PEG polymeric mixture to complex siGL3 increases with the increase in the EGF-based component in the EP-ICONs and, for each sample, with the increase in the copolymer:siRNA weight ratio (R). On the other hand, the susceptibility of loaded siRNA towards RNase decreases with the increase in the pegylated component in the polymeric mixture. At all R values, the average size and the zeta potential values are suitable for escap...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research