Synergistic erythropoietic mechanisms of Chromolaena odorata and Tithonia diversifolia in the bone marrow of Wistar rats

This study aimed to elucidate the scientific mechanisms that underpin the traditional use ofChromolaena odorata andTithonia diversifolia as remedy for paediatric anaemia. We investigated the impact of these plants on erythropoietin and erythropoietin receptor expression and monitored cyclin-dependent kinase inhibitors, and FasL/FAS signalling mechanism therefrom. Three samples were prepared from spouting leaves ofC. odorata and leaves of floweringT. diversifolia as follows: C.O-group (100gC. odorata was blended in 100 ml distilled/deionized water (dd-water)), T.D-group (100gT. diversifolia was blended in 100 ml dd-water) and the T.D + C.O-group (50g each ofC. odorata andT. diversifolia were blended in 100 ml dd-water). Eight-week oldin-bred Wistar rats (female) were grouped into 4 (n=3): basal control (2 ml of dd-water), C.O-group (30 mg/kgb.w.), T.D-group (30 mg/kgb.w.) and T.D + C.O-group (30 mg/kgb.w). An oral dose of the extracts was administered 6-h for 72 h followed by RT-PCR analysis of erythropoietin (Epo), erythropoietin receptor (Epo-R), kip1.p27 (p27/cdkn1b), p21Waf1, Kip2-p57 and FAS/FASL. Western blot was used to investigate JAK2 phosphorylationin vitro in bone marrow primary culture. The administration ofC. odorata andT. diversifolia potentiated the upregulation of erythropoietin. This was associated synergistically with an upshot of bone marrow erythropoietin receptor while p21Waf1, Kip2-p57 were downregulated in treatment groups. However, the combination ofC. ...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research