Rbm46, a novel germ cell specific factor, modulates meiotic progression and spermatogenesis.

Rbm46, a novel germ cell specific factor, modulates meiotic progression and spermatogenesis.
Biol Reprod. 2021 Feb 02;:
Authors: Dai X, Cheng X, Huang J, Gao Y, Wang D, Feng Z, Zhai G, Lou Q, He J, Wang Z, Yin Z
Abstract
nesis but few has been functionally verified. Here, we identified one member, Rbm46 and investigated its expression and role during zebrafish reproduction. We found that is maternally provided and specifically expressed in the germ cells of gonadal tissues using in situ hybridization, RT-PCR and qRT-PCR. Utilizing TALEN (transcription activator-like effector nuclease) techniques, two independent rbm46 mutant zebrafish lines were generated. Specific disruption of rbm46 resulted in masculinization and infertility in the mutants. Although the spermatogonia appeared grossly normal, spermatogenesis was impaired and meiosis events were not observed in the mutants. The introduction of a tp53M214K mutation could not rescue the female-to-male sex-reversal phenotype, which suggests that rbm46 acts independently of p53 dependent apoptotic pathway. RNA-seq and qRT-PCR results subsequently indicate that Rbm46 might be involved in post-transcriptional regulation of functional genes essential for the germ cell development such as nanos3, dazl, sycp3 during gametogenesis. Altogether, our results firstly reveal a crucial in vivo role of rbm46 in regulating germ cell development by promoting the transition from mitosis to meiosis.
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Source: Biology of Reproduction - February 2, 2021 Category: Reproduction Medicine Authors: Dai X, Cheng X, Huang J, Gao Y, Wang D, Feng Z, Zhai G, Lou Q, He J, Wang Z, Yin Z Tags: Biol Reprod Source Type: research