Development and validation of an analysis method for pesticide residues by gas chromatography –tandem mass spectrometry in Daikenchuto

In this study, we developed an analytical method for 91 compounds, which are listed in USP, using DKT as the subject. The method could extract pesticides from DKT with acetone, elute pesticides with acetonitrile using a SepPak C18 column (5 g) and with ethyl acetate using a DSC-NH2 column (2  g), and perform simultaneous analyses by gas chromatography–tandem mass spectrometry (GC–MS/MS). This method, which could quantify 88 compounds, was validated according to USP. A pesticide residue analysis method that meets USP requirements enables the analysis of pesticide residues with a hig h residue risk and contributes to improving the safety of “Kampo” and other herbal medicines.
Source: Journal of Natural Medicines - Category: Drugs & Pharmacology Source Type: research