Episomal Reprogramming of Human Peripheral Blood Mononuclear Cells into Pluripotency.

Episomal Reprogramming of Human Peripheral Blood Mononuclear Cells into Pluripotency. Methods Mol Biol. 2021;2239:117-133 Authors: Wen W, Cheng T, Zhang XB Abstract Peripheral blood is an easily accessible cell resource for reprogramming into pluripotency by episomal vectors. Here, we describe an approach for efficient generation of integration-free induced pluripotent stem cells (iPSCs) under feeder or feeder-free conditions. Additionally, in combination with the CRISPR-Cas9 genome-editing system, we can directly generate edited iPSCs from blood cells. With this protocol, one can easily generate either integration-free iPSCs or genetically edited iPSCs from peripheral blood at high efficiency. PMID: 33226616 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research