The Ubiquitin Ligase RNF8 Regulates Rho GTPases and Promotes Cytoskeletal Changes and Motility in Triple ‐Negative Breast Cancer Cells

In this study, we investigated the role of RNF8 in regulating Rho GTPases and cell motility. We find that RNF8 knockdown in TNBC ce lls attenuates the protein and mRNA levels of RHOA and CDC42. We show that the formation of filopodia, focal adhesions, and the association of focal adhesions to stress fibers is impaired upon RNF8 knockdown. Cell migration is significantly inhibited by RNF8 knockdown. Our study suggests a potential novel role for RNF8 in mediating cell migration in TNBC through regulation of the Rho GTPases RHOA and CDC42.
Source: FEBS Letters - Category: Biochemistry Authors: Tags: RESEARCH LETTER Source Type: research