Creation of the first monoclonal antibody recognizing an extracellular epitope of hABCC6

AbstractMutations in theABCC6 gene result in calcification diseases such as pseudoxanthoma elasticum or Generalized Arterial Calcification of Infancy. Generation of antibodies recognizing an extracellular epitope of ABCC6 has been hampered by the short extracellular segments of the protein. To overcome this limitation, we immunized Bovine FcRn transgenic mice exhibiting an augmented humoral immune response with HEK cells expressing human ABCC6 (hABCC6). We obtained a monoclonal antibody recognizing an extracellular epitope of hABCC6 that we named mEChC6. Limited proteolysis revealed that the epitope is within a loop in the N ‐terminal half of ABCC6 and probably spans amino acids 338‐347. mEChC6 recognizes hABCC6 in the liver of hABCC6 transgenic mice, verifying both specificity and extracellular binding to intact hepatocytes.

Source: FEBS Letters - November 7, 2020 Category: Biochemistry Authors: E. Koz ák, B. Szikora, A. Iliás, P.K. Jani, Z. Hegyi, Zs. Matula, D. Dedinszki, N. Tőkési, K. Fülöp, V. Pomozi, Gy. Várady, É. Bakos, G.E. Tusnády, I. Kacskovics, A. Váradi Tags: RESEARCH LETTER Source Type: research