Structural analysis of the core and polysaccharide from the lipopolysaccharide produced by Chromobacterium violaceum strain ATCC 12472 (NCTC 9757).

Structural analysis of the core and polysaccharide from the lipopolysaccharide produced by Chromobacterium violaceum strain ATCC 12472 (NCTC 9757). Carbohydr Res. 2020 Oct 22;498:108182 Authors: Vinogradov E, Lindner B, Holst O Abstract The structure of the polysaccharide O-chain of the lipopolysaccharide isolated from the sequenced strain Chromobacterium violaceum ATCC 12472 (NCTC 9757) was investigated by chemical and NMR analyses, and concluded to be -4-α-Leg5Ac7Ala-4-β-d-ManNAlaA3OAc-3-α-d-GlcNAc-where Leg5Ac7Ala indicates 5-acetamido-7-alanylamido-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulopyranosonic acid and ManNAlaA3OAc 3-O-acetyl-2-alanylamido-2-deoxymannopyranuronic acid. The structure of the core with one repeating unit of the polysaccharide attached was also analyzed, and it was found that the O-chain polysaccharide is linked to the core via β-GlcpNAc, as opposite to α-GlcpNAc inside the O-chain. PMID: 33137586 [PubMed - as supplied by publisher]
Source: Carbohydrate Research - Category: Genetics & Stem Cells Authors: Tags: Carbohydr Res Source Type: research