[Evaluation of the Two Commercial Methods Based on Broth Microdilution Against Reference Microdilution Method for Klebsiella pneumoniae Isolates].

[Evaluation of the Two Commercial Methods Based on Broth Microdilution Against Reference Microdilution Method for Klebsiella pneumoniae Isolates]. Mikrobiyol Bul. 2020 Oct;54(4):606-612 Authors: Kansak N, Aslan M, Adaleti R, Aksaray S Abstract A rapid and reliable method for antimicrobial susceptibility test of colistin is needed because of increasing numbers of multi-resistant gram negative bacterial infections and simultaneus increasing of colistin resistance. Although broth microdilution (BMD) is recommended by the Clinical and Laboratory Standards Institute (CLSI) and the European Committee for Antimicrobial Susceptibility Testing (EUCAST) as a reference method, the use in routine laboratory practice is limited because of the difficulties in application and time-consuming characteristics. Recently, many BMD based commercial products were developed. The study was aimed to compare the results of the two commercial systems available for the detection of colistin sensitivity with the reference method. Totally 38 Klebsiella pneumoniae strains isolated from various clinical specimens between 2017-2018 were included in our study. Identification and antibiotic susceptibility tests were performed with "matrix-assisted laser desorption/ionization timeof-flight, mass spectrometry (MALDI-TOF MS)" and Vitek-2 (bioMérieux, Marcyl'Etoile, France) systems. BMD tests were performed with Sensititre (Sensititre custom plate, Thermo Fisher Scientif...
Source: Mikrobiyoloji Bulteni - Category: Microbiology Tags: Mikrobiyol Bul Source Type: research