Changes in the extracted amounts and seasonally variable constituents of Diospyros kaki at different growth stages

In this study, by focusing on the medicinal part of Persimmon Calyx, calyx on mature fruit ofD. kaki, we examined the changes in the extraction amounts of 3 cultivars ofD. kaki ( ‘Hiratanenashi’, ‘Jiro’, and ‘Tonewase’) to identify and quantify seasonally variable constituents during the maturation process by analysing their chemical compositions. We found that the extraction weight of the calyx, fruit of persimmons, and total tannin content in calyxes were signi ficantly increased during maturation. Lupeol (1), betulinic acid (2), pomolic acid (3), ursolic acid (4), β-sitosterol (5), rotungenic acid (6), barbinervic acid (7), catechin (8), gallocatechin (9), and sucrose (10) were identified in the calyx ofD. kaki. Compounds1,6, and7 were isolated from Persimmon Calyx for the first time. Moreover, the isolated compounds (1–7) and their analogue (oleanolic acid) were quantitatively analysed, and the results showed that the amounts of4 and oleanolic acid were reduced during maturation, whereas that of2,3,6, and7 were increased.
Source: Journal of Natural Medicines - Category: Drugs & Pharmacology Source Type: research