Chemotypic variation of non-volatile constituents of Artemisia afra (African wormwood) from South Africa.

Chemotypic variation of non-volatile constituents of Artemisia afra (African wormwood) from South Africa. Fitoterapia. 2020 Oct 08;:104740 Authors: Sotenjwa VZ, Chen W, G L Veale C, Anokwuru CP, Tankeu S, Combrinck S, Kamatou GPP, Viljoen AM Abstract Artemisia afra, (African wormwood) is a popular medicinal plant of southern Africa and is an excellent candidate for commercialisation. This current study was aimed at exploring the phytochemistry and chemical variation of non-volatile compounds within wild populations of A, afra, and developing chromatographic quality control protocols for raw materials based on the identification of marker compounds. Chromatographic data, from samples representing 12 distinct populations were obtained using liquid chromatography-mass spectrometry. An untargeted chemometric approach revealed three clusters. Marker compounds for each cluster, revealed through discriminant analysis, were isolated and identified using NMR spectroscopy, as acacetin (1) (Group 1), chrysoeriol (2) (Group 2), and 3.5-di-O-caffeoylquinic acid (3) and scopoletin (4) (Group 3). In addition, (3) and rutin (5), (both reported for the first time from A. afra), and (1), (2), (4) and 4-caffeoylquinic acid (6) were established as reliable markers for species identification, since they were abundant in most samples. Quantitative analysis usingvalidated method established (4) as the dominant compound in the samples (1080-19,600 μg/g d...
Source: Fitoterapia - Category: Biochemistry Authors: Tags: Fitoterapia Source Type: research