Screening dietary fibres for fermentation characteristics and metabolic profiles using a rapid in vitro approach: implications for irritable bowel syndrome.

Screening dietary fibres for fermentation characteristics and metabolic profiles using a rapid in vitro approach: implications for irritable bowel syndrome. Br J Nutr. 2020 Oct 08;:1-35 Authors: So D, Yao CK, Gill PA, Pillai N, Gibson PR, Muir JG Abstract The therapeutic value of specific fibres is partly dependent on their fermentation characteristics. Some fibres are rapidly degraded with generation of gases that induce symptoms in patients with irritable bowel syndrome (IBS), while more slowly- or non-fermentable fibres may be more suitable. More work is needed to profile a comprehensive range of fibre to determine suitability for IBS. Using a rapid in vitro fermentation model, gas production and metabolite profiles of a range of established and novel fibres were compared. Fibre substrates (n=15) were added to faecal slurries from 3 healthy donors for 4 hours with gas production measured using real-time headspace sampling. Concentrations of short-chain fatty acids (SCFA) and ammonia were analysed using gas chromatography and enzymatic assay respectively. Gas production followed 3 patterns: rapid (≥60 mL/g over 4 hours) for fructans, carrot fibre and corn-derived xylo-oligosaccharide; mild (30-60 mL/g) for partially hydrolysed guar gum, almond shell-derived xylo-oligosaccharide and one type of high-amylose resistant starch 2 (RS2); and minimal (no differences with blank controls) for methylcellulose, another high-amylose RS2, ace...
Source: The British Journal of Nutrition - Category: Nutrition Authors: Tags: Br J Nutr Source Type: research