GSE158225 Differentially expressed genes in rough and smooth colony isolates of Clostridioides difficile

Contributors : Elizabeth M Garrett ; Rita TamayoSeries Type : Expression profiling by high throughput sequencingOrganism : Clostridioides difficile R20291Purpose: to determine the differentially expressed genes in the phase-variable rough and smooth colony isolates of C. difficileMethods: C. difficile R20291 was grown on BHIS agar to obtain distinct colonies. Individual rough and smooth colonies were chosen for propagation on BHIS-agar for 24 hours as described in Garrett, et al., PLoS Biology, 2019. Growth was collected from n = 2 biological replicates. RNA was purified using TriSure and chloroform, beadbeating, and isopropanol/ethanol precipitation. Quality was verified with Bioanalyzer Assay. Samples were submitted to Genewiz for depletion of rRNA using the TruSeq RiboZero Gold Kit, library preparation, and single-end sequencing on the Illumina HiSeq 2500 platform. RNA sequencing analysis was done using CLC Genomic Workbench v20. Reads were mapped to C. difficile R20291 genome using the software's default menalties for mismatch, deletion, and insertion differences from the reference genome. Transcript reads were normalized as RPKM.Results: 88 genes were differentially expressed between bacteria from rough versus smooth colonies, with equal to or greater than a 2-fold change and p
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Clostridioides difficile R20291 Source Type: research

Related Links:

Clostridioides difficile infection rates in hospitalized hematopoietic stem cell transplant (HSCT) and hematologic malignancy (HM) patients are higher than in the general population. This is related both to extensive exposure to antibiotics as well as to frequent and often prolonged hospitalization. In this population, with numerous potential causes for diarrhea, a subset of C. difficile detection is presumed to represent colonization rather than clinical infection. Use of decision support tools to guide ordering in hospitalized patients has been reported to decrease both C.
Source: Biology of Blood and Marrow Transplantation - Category: Hematology Authors: Source Type: research
Publication date: Available online 5 October 2020Source: American Journal of Infection ControlAuthor(s): Enrico Bentivegna, Giuliano Alessio, Valerio Spuntarelli, Michelangelo Luciani, Iolanda Santino, Maurizio Simmaco, Paolo Martelletti
Source: American Journal of Infection Control - Category: Infectious Diseases Source Type: research
TUESDAY, Oct. 6, 2020 -- Fecal microbiota transplantation (FMT) is effective for Clostridium difficile infection (CDI) through six months and has a good safety profile, according to a study published online Oct. 1 in Gastroenterology. Colleen R....
Source: Drugs.com - Pharma News - Category: Pharmaceuticals Source Type: news
Abstract The relationship between the gut microbiota and Clostridioides difficile, and its role in the severity of C. difficile infection in humans is an area of active research. Intestinal carriage of toxigenic and non-toxigenic C. difficile strains, with and without clinical signs, is reported in animals, however few studies have looked at the risk factors associated with C. difficile carriage and the role of the host gut microbiota. Here, we isolated and characterized C. difficile strains from different animal species (predominantly canines (dogs), felines (cats), and equines (horses)) that were brought in for ...
Source: Anaerobe - Category: Microbiology Authors: Tags: Anaerobe Source Type: research
Conclusion: Diabetes mellitus, prior antibiotic treatment, hematological malignancy on chemotherapy, malnutrition, neutropenia and Ryle feeding are risk factors for development of CDAD. Tight restriction of unnecessary antibiotic uses is necessary in our locality. Direct detection of toxin genes in stool by PCR is sensitive and specific method for early detection of C. difficile. PMID: 32994900 [PubMed]
Source: Iranian Journal of Microbiology - Category: Microbiology Tags: Iran J Microbiol Source Type: research
Ninety percent of patients treated with fecal microbiota transplantation recovered from C. difficile infections, with few serious adverse events, a study of registry data found.Medscape Medical News
Source: Medscape Gastroenterology Headlines - Category: Gastroenterology Tags: Gastroenterology News Source Type: news
This study is the first to characterize aspects of the origin region of C. difficile and contributes to our understanding of the initiation of DNA replication in clostridia.
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Proton pump inhibitors (PPIs) and histamine 2 receptor antagonists (H2RAs) are effective medications for gastric acid suppression. Because of their ubiquity and lack of significant short-term side effects, there is evidence of overprescribing PPIs and H2RAs when they are not clinically indicated. There are side effects associated with PPIs and H2RAs including Clostridium difficile (C. diff) infection and community acquired pneumonia (CAP), and thus efforts should be made to decrease inappropriate prescribing.
Source: Journal of the American College of Surgeons - Category: Surgery Authors: Tags: General Surgery Source Type: research
Clostridioides difficile infection (CDI) is an increasingly common and life-threatening infection. We sought to determine why certain SICU rooms in an academic tertiary hospital harbored a majority of CDI in the past 9 years.
Source: Journal of the American College of Surgeons - Category: Surgery Authors: Tags: Trauma Source Type: research
Fecal microbiota transplantation (FMT) is commonly used for treatment of C. difficile infections (CDI), although prospective safety data are limited and real-world FMT practice and outcomes are not well described. The FMT National Registry was designed to assess FMT methods and both safety and effectiveness outcomes from North American FMT providers.
Source: Gastroenterology - Category: Gastroenterology Authors: Source Type: research
More News: Biology | Clostridium Difficile | Databases & Libraries | Genetics | Men