Comparing DADA2 and OTU clustering approaches in studying the bacterial communities of atopic dermatitis.

Conclusion. We believe that DADA2 represents an improvement over an OTU clustering approach, and that biological replication rather than technical replication is a more effective use of resources. However, neither OTU clustering nor DADA2 gave insights into Staphylococcus community dynamics, and caution should remain in not overinterpreting the taxonomic assignments at lower taxonomic ranks.
PMID: 32965212 [PubMed - as supplied by publisher]

Source: Journal of Medical Microbiology - September 22, 2020 Category: Microbiology Authors: Barnes CJ, Rasmussen L, Asplund M, Knudsen SW, Clausen ML, Agner T, Hansen AJ Tags: J Med Microbiol Source Type: research