Multilocus sequence typing of Candida albicans isolates from Burn Intensive Care Unit (BICU) in Iran.

Multilocus sequence typing of Candida albicans isolates from Burn Intensive Care Unit (BICU) in Iran. J Med Microbiol. 2015 Jan 16; Authors: Afsarian MH, Badali H, Boekhout T, Shokohi T, Katiraee F Abstract Burn intensive care unit patients are specifically exposed to deep-seated nosocomial infections caused by Candida albicans. Superficial carriage of C. albicans is a potential source of infection and dissemination, and typing methods could be useful to trace the different isolates. Multilocus sequence typing is a powerful genotyping method for pathogenic microorganisms, including Candida albicans. Thirty clinical isolates of C. albicans obtained from 22 patients that admitted to the Burn Intensive Care Unit (BICU) from a burn hospital at Sari, Mazandaran state, Iran were epidemiologically studied by multilocus sequence typing (MLST). Seventy five variable nucleotide sites were found. Sixty two alleles were identified among the seven loci of the C. albicans isolates and one new allele was obtained. Eighteen Diploid Sequence Types (DSTs) were identified and among those 10 were new. These isolates belonged to nine clonal clusters (CCs) while two isolates occurred as singletons. Eleven (36.7%) isolates belonged to CC 124 after eBURST analysis and 13 isolates (43.3%) were assigned to clade 4. Approximately 17% of the 30 isolates belonged to clade 1 (CC 69 and CC766). Isolates from several patients with burns were found to be genetically...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research