Evaluation of the diagnostic accuracy of an affordable rapid diagnostic test for African Swine Fever antigen detection in Lao People's Democratic Republic.

In this study, an ASF antigen detection RDT from Shenzhen Lvshiyuan Biotechnology Co. Ltd was evaluated using clinical field samples submitted to the National Animal Health Laboratory (NAHL) from ASF suspect cases between June and December 2019 in Lao PDR. Positive (n = 57) and negative (n = 50) samples of whole blood, serum and haemolysed serum were assessed by RDT and PCR, with the latter used as the gold standard reference comparator. Overall the RDT had a diagnostic sensitivity (DSe) of 65%, 95% CI [51 - 77] and diagnostic specificity (DSp) of 76%, 95% CI [62 - 87]. The RDT demonstrated improved performance on samples with lower PCR cycle threshold (ct) values with each additional cycle reducing the odds of the RDT returning a positive by 17% relative to the previous cycle, 95% CI [8% - 28%] (P < 0.01). While this test shows promise for field application, complete validation of diagnostic accuracy requires a larger sample size. PMID: 32956709 [PubMed - as supplied by publisher]
Source: Journal of Virological Methods - Category: Virology Authors: Tags: J Virol Methods Source Type: research