Structure of the Plasmodium falciparum PfSERA5 pseudo ‐zymogen

In this study we present a revised structure of the central PfSERA5E domain at a resolution of 1.2 å, and the first structure of the “zymogen” of this papain‐like domain including its cognate prodo main (PfSERA5PE) to 2.2 å resolution. PfSERA5PE is somewhat structurally similar to that of other known proenzymes, retaining the conserved overall folding and orientation of the prodomain through, and occluding, the archetypal papain‐like catalytic triad “active‐site” cleft, in the same reverse direction as conventional prodomains. Our findings are congruent with previously identified structures of PfSERA5E and of similar “zymogens” and provide a foundation for further investigation into the function of PfSERA5.

Source: Protein Science - September 20, 2020 Category: Biochemistry Authors: Nicholas A. Smith, Oliver B. Clarke, Mihwa Lee, Anthony N. Hodder, Brian J. Smith Tags: Article Source Type: research