Sensors, Vol. 20, Pages 5275: Robust Magnetized Graphene Oxide Platform for In Situ Peptide Synthesis and FRET-Based Protease Detection
Sensors, Vol. 20, Pages 5275: Robust Magnetized Graphene Oxide Platform for In Situ Peptide Synthesis and FRET-Based Protease Detection
Sensors doi: 10.3390/s20185275
Authors:
Seongsoo Kim
Sang-Myung Lee
Je Pil Yoon
Namhun Lee
Jinhyo Chung
Woo-Jae Chung
Dong-Sik Shin
Graphene oxide (GO)/peptide complexes as a promising disease biomarker analysis platform have been used to detect proteolytic activity by observing the turn-on signal of the quenched fluorescence upon the release of peptide fragments. However, the purification steps are often cumbersome during surface modification of nano-/micro-sized GO. In addition, it is still challenging to incorporate the specific peptides into GO with proper orientation using conventional immobilization methods based on pre-synthesized peptides. Here, we demonstrate a robust magnetic GO (MGO) fluorescence resonance energy transfer (FRET) platform based on in situ sequence-specific peptide synthesis of MGO. The magnetization of GO was achieved by co-precipitation of an iron precursor solution. Magnetic purification/isolation enabled efficient incorporation of amino-polyethylene glycol spacers and subsequent solid-phase peptide synthesis of MGO to ensure the oriented immobilization of the peptide, which was evaluated by mass spectrometry after photocleavage. The FRET peptide MGO responded to proteases such as trypsin, thrombin, and β-secretase in a concentration-dependent manner. Particularly, β-s...
Source: Sensors - Category: Biotechnology Authors: Seongsoo Kim Sang-Myung Lee Je Pil Yoon Namhun Lee Jinhyo Chung Woo-Jae Chung Dong-Sik Shin Tags: Letter Source Type: research