Evaluation of Two Commercial Broth Microdilution Methods Using Different Interpretive Criteria for the Detection of Molecular Mechanisms of Acquired Azole and Echinocandin Resistance in Four Common Candida Species.

Evaluation of Two Commercial Broth Microdilution Methods Using Different Interpretive Criteria for the Detection of Molecular Mechanisms of Acquired Azole and Echinocandin Resistance in Four Common Candida Species. Antimicrob Agents Chemother. 2020 Sep 08;: Authors: Lim HJ, Shin JH, Kim MN, Yong D, Byun SA, Choi MJ, Lee SY, Won EJ, Kee SJ, Kim SH, Shin MG Abstract The abilities of the new Vitek 2 AST-YS08 (YS08) and Sensititre YeastOne (SYO) systems to detect resistances of Candida isolates to azoles and echinocandins were evaluated. In total, 292 isolates including 28 Candida albicans (6 Erg11 and 2 Fks mutants), 57 Candida parapsilosis (26 Erg11 mutants), 24 Candida tropicalis (10 Erg11 and 1 Fks mutants), and 183 Candida glabrata (39 Pdr1 and 13 Fks mutants) isolates were tested. Categorical agreements (CAs) between Clinical and Laboratory Standards Institute (CLSI) and YS08 fluconazole MICs using clinical breakpoints were 92.4% (C. albicans), 96.5% (C. parapsilosis), and 87.0% (C. tropicalis) and between CLSI and SYO MICs were 92.3% (C. albicans), 77.2% (C. parapsilosis), 100% (C. tropicalis), and 98.9% (C. glabrata). For C. glabrata, CAs with the CLSI micafungin were 92.4% and 55.5% for YS08 micafungin and caspofungin, respectively; they were 100%, 95.6%, and 98.9% for SYO micafungin, caspofungin, and anidulafungin, respectively. YS08 does not provide fluconazole data for C. glabrata; the CA with the CLSI fluconazole was 97.8% f...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research