Overcoming Symmetry Mismatch in Vaccine Nanoassembly via Spontaneous Amidation.
Overcoming Symmetry Mismatch in Vaccine Nanoassembly via Spontaneous Amidation. Angew Chem Int Ed Engl. 2020 Sep 04;: Authors: Howarth M, Rahikainen R, Rijal P, Tan T, Draper S, Townsend A, Wu HJ, Bowden T, Andersson AM, Barrett J Abstract Matching of symmetry at interfaces is a fundamental obstacle in molecular assembly. Virus-like particles (VLPs) are important platforms to generate vaccines against pathogenic threats including Covid-19. However, symmetry mismatch between multimeric antigens and VLP subunits can prohibit vaccine nanoassembly. Here we explore spontaneous amidation to establish principles for coupling VLPs to diverse antigen symmetries. SpyTag003/SpyCatcher003-mediated decoration enabled efficient VLP conjugation and extreme thermal resilience. Many people showed pre-existing antibodies to SpyTag:SpyCatcher, but decreased antibodies against 003 variants. We coupled efficiently to the computationally-designed VLP, built from twenty trimers, not only monomers (SARS-CoV-2) but also cyclic dimers (Newcastle Disease Virus or Lyme disease), trimers (Influenza Hemagglutinins), and tetramers (Influenza Neuraminidases). Even small antigens with dihedral symmetry could be displayed. For the global challenge of Influenza, Spy-display enabled powerful induction of neutralizing antibodies to trimeric and tetrameric antigens. SpyCatcher003 conjugation is compatible with nanodisplay of diverse symmetries, towards generation of potent vaccines. PMI...
Publication date: Available online 10 October 2020Source: American Journal of Kidney DiseasesAuthor(s): Shreeram Akilesh, Cynthia C. Nast, Michifumi Yamashita, Kammi Henriksen, Vivek Charu, Megan L. Troxell, Neeraja Kambham, Erika Bracamonte, Donald Houghton, Naila I. Ahmed, Chyi Chyi Chong, Bijin Thajudeen, Shehzad Rehman, Firas Khoury, Jonathan E. Zuckerman, Jeremy Gitomer, Parthassarathy C. Raguram, Shanza Mujeeb, Ulrike Schwarze, M. Brendan Shannon
Publication date: Available online 9 October 2020Source: Reumatología Clínica (English Edition)Author(s): Lina María Saldarriaga Rivera, Daniel Fernández Ávila, Wilson Bautista Molano, Daniel Jaramillo Arroyave, Alain Jasaf Bautista Ramírez, Adriana Díaz Maldonado, Jorge Hernán Izquierdo, Edwin Jáuregui, María Constanza Latorre Muñoz, Juan Pablo Restrepo, Juan Sebastián Segura Charry
CONCLUSIONS: This single practice study showed total patient contact was similar over both sample periods, but most contact in 2020 was virtual. Further longitudinal multi-practice studies to confirm these findings and describe future consultation patterns are needed to inform general practice service delivery post-COVID-19. PMID: 33032304 [PubMed - in process]
Publication date: Available online 1 October 2020Source: Academic RadiologyAuthor(s): Neo Poyiadji, Chad Klochko, Jeff LaForce, Manuel L. Brown, Brent Griffith
Publication date: 15 February 2021Source: Personality and Individual Differences, Volume 170Author(s): Brian W. Haas, Fumiko Hoeft, Kazufumi Omura
Authors: Hui KK PMID: 33034297 [PubMed - as supplied by publisher]
Authors: Lam PT PMID: 33034296 [PubMed - as supplied by publisher]
Publication date: Available online 9 October 2020Source: Neurología (English Edition)Author(s): G. Alvarez Bravo, L. RamióTorrentà
Publication date: Available online 9 October 2020Source: Neurología (English Edition)Author(s): N. Morollón, R. Belvís, A. De Dios, N. Pagès, C. González-Oria, G. Latorre, S. Santos-Lasaosa
Publication date: Available online 9 October 2020Source: Neurología (English Edition)Author(s): M.J. Abenza-Abildúa, M.T. Ramírez-Prieto, R. Moreno-Zabaleta, N. Arenas-Valls, M.A. Salvador-Maya, C. Algarra-Lucas, B. Rojo Moreno-Arrones, B. Sánchez-Cordón, J. Ojeda-Ruíz de Luna, C. Jimeno-Montero, F.J. Navacerrada-Barrero, C. Borrue-Fernández, E. Malmierca-Corral, P. Ruíz-Seco, P. González-Ruano, I. Palmí-Cortés, J. Fernández-Travieso, M. Mata-Álvarez de Santullano, M.L. Almarcha-Menargues, G. Gutierrez-Gutierrez