Overcoming Symmetry Mismatch in Vaccine Nanoassembly via Spontaneous Amidation.
Overcoming Symmetry Mismatch in Vaccine Nanoassembly via Spontaneous Amidation. Angew Chem Int Ed Engl. 2020 Sep 04;: Authors: Howarth M, Rahikainen R, Rijal P, Tan T, Draper S, Townsend A, Wu HJ, Bowden T, Andersson AM, Barrett J Abstract Matching of symmetry at interfaces is a fundamental obstacle in molecular assembly. Virus-like particles (VLPs) are important platforms to generate vaccines against pathogenic threats including Covid-19. However, symmetry mismatch between multimeric antigens and VLP subunits can prohibit vaccine nanoassembly. Here we explore spontaneous amidation to establish principles for coupling VLPs to diverse antigen symmetries. SpyTag003/SpyCatcher003-mediated decoration enabled efficient VLP conjugation and extreme thermal resilience. Many people showed pre-existing antibodies to SpyTag:SpyCatcher, but decreased antibodies against 003 variants. We coupled efficiently to the computationally-designed VLP, built from twenty trimers, not only monomers (SARS-CoV-2) but also cyclic dimers (Newcastle Disease Virus or Lyme disease), trimers (Influenza Hemagglutinins), and tetramers (Influenza Neuraminidases). Even small antigens with dihedral symmetry could be displayed. For the global challenge of Influenza, Spy-display enabled powerful induction of neutralizing antibodies to trimeric and tetrameric antigens. SpyCatcher003 conjugation is compatible with nanodisplay of diverse symmetries, towards generation of potent vaccines. PMI...
Authors: Leung TI, Biskup E, DeWitt D Abstract CONTEXT: Physicians who migrate globally face a daunting series of time-consuming, labor- and resource-intensive procedures to prove their clinical competency before being allowed to practice medicine in a new country. ISSUES: In this commentary, we describe licensing barriers faced by physician-migrants based on the authors' experiences, and reflect also on rapidly implemented measures to address COVID-19 pandemic related workforce shortages. We offer recommendations for potential reductions in bureaucratic regulatory barriers that prohibit mobilization of interna...
Authors: Kim Y, Sung HK, Bang JH, Koh IS, Joh JS, Ju YS, Min HS, Chin BS, Chung KH PMID: 32989937 [PubMed - in process]
Authors: Lee YM, Park KD, Seo JH PMID: 32989936 [PubMed - in process]
CONCLUSION: These data support a link between TLR4 signaling and pathological inflammation during COVID-19 and contribute to develop therapeutic approaches through targeting TLR4-mediated inflammation. PMID: 32989935 [PubMed - in process]
Authors: Song SK, Lee DH, Cho MR PMID: 32989934 [PubMed - in process]
Authors: Lee J, Kim SY, Sung H, Choe YJ, Hong KH PMID: 32989933 [PubMed - in process]
CONCLUSION: Vaccine-related anaphylaxis seems to have been very rare in the past, but health care professionals must always be aware of anaphylaxis. PMID: 32989932 [PubMed - in process]
CONCLUSION: After outpatient episode incidence of influenza, antiviral treatment has been shown to reduce the incidence of hospitalized pneumonia, especially in infants and children, during pandemic season 2017. Antiviral therapy for influenza is recommended to minimize burden caused by influenza virus infection and to reduce pneumonia. In addition, medical costs of hospitalization may decrease by antiviral therapy, especially in infants and children. PMID: 32989929 [PubMed - in process]
Publication date: October 2020Source: Journal of Clinical Neuroscience, Volume 80Author(s): Daniel Águila-Gordo, José Manuel Flores-Barragán, Ferran Ferragut-Lloret, Jaime Portela-Gutierrez, Beatriz LaRosa-Salas, Lourdes Porras-Leal, José Carlos Villa Guzmán